Experiment set6H35 for Pseudomonas stutzeri RCH2

Compare to:

RCH2 defined media with Cisplatin 0.0002 mg/ml

Group: stress
Media: RCH2_defined_no_vitamin + Cisplatin (2e-04 mg/ml), pH=7.2
Culturing: psRCH2_ML7, 48 well microplate; Tecan Infinite F200, Aerobic, at 30 (C), shaken=orbital
By: Kelly on 8/12/2013
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 20 mM Sodium D,L-Lactate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate)
Growth plate: 626 E5,E6

Specific Phenotypes

For 10 genes in this experiment

For stress Cisplatin in Pseudomonas stutzeri RCH2

For stress Cisplatin across organisms

SEED Subsystems

Subsystem #Specific
DNA-replication 4
DNA repair, UvrABC system 3
DNA repair, bacterial RecFOR pathway 3
DNA repair, bacterial 1
Queuosine-Archaeosine Biosynthesis 1
Transcription factors bacterial 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes: