Experiment set6H12 for Shewanella oneidensis MR-1

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LB with 1-ethyl-3-methylimidazolium acetate 20 mM

Group: stress
Media: LB + 1-ethyl-3-methylimidazolium acetate (20 mM)
Culturing: MR1_ML3, 48 well microplate; Tecan Infinite F200, Aerobic, at 30 (C), shaken=orbital
By: Adam on 10/22/2013
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 755 A3,A4

Specific Phenotypes

For 29 genes in this experiment

For stress 1-ethyl-3-methylimidazolium acetate in Shewanella oneidensis MR-1

For stress 1-ethyl-3-methylimidazolium acetate across organisms

SEED Subsystems

Subsystem #Specific
Ammonia assimilation 1
Biotin biosynthesis 1
Campylobacter Iron Metabolism 1
Carboxysome 1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis 1
Lactose and Galactose Uptake and Utilization 1
Lipid A modifications 1
Respiratory dehydrogenases 1 1
n-Phenylalkanoic acid degradation 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-glutamate biosynthesis I 2 2 2
long-chain fatty acid activation 1 1 1
L-glutamine degradation I 1 1 1
L-glutamine degradation II 1 1 1
ammonia assimilation cycle III 3 3 2
NADH to cytochrome bd oxidase electron transfer I 2 2 1
glutathione degradation (DUG pathway) 2 2 1
NADH to cytochrome bd oxidase electron transfer II 2 2 1
NADH to nitrate electron transfer 2 1 1
NADH to cytochrome aa3 oxidase electron transfer 2 1 1
γ-linolenate biosynthesis II (animals) 2 1 1
linoleate biosynthesis II (animals) 2 1 1
NADH to cytochrome bo oxidase electron transfer I 2 1 1
NADH to cytochrome bo oxidase electron transfer II 2 1 1
nitrate reduction VIIIb (dissimilatory) 2 1 1
D-galactose detoxification 3 2 1
aerobic respiration III (alternative oxidase pathway) 3 2 1
3-methyl-branched fatty acid α-oxidation 6 3 2
oleate biosynthesis I (plants) 3 1 1
alkane biosynthesis II 3 1 1
L-glutamate and L-glutamine biosynthesis 7 6 2
S-methyl-5-thio-α-D-ribose 1-phosphate degradation I 7 2 2
phytol degradation 4 3 1
aerobic respiration I (cytochrome c) 4 3 1
aerobic respiration II (cytochrome c) (yeast) 4 3 1
phosphatidylcholine acyl editing 4 2 1
long chain fatty acid ester synthesis (engineered) 4 1 1
glutaminyl-tRNAgln biosynthesis via transamidation 4 1 1
muropeptide degradation 4 1 1
wax esters biosynthesis II 4 1 1
L-asparagine biosynthesis III (tRNA-dependent) 4 1 1
sporopollenin precursors biosynthesis 18 5 4
D-galactose degradation I (Leloir pathway) 5 4 1
mitochondrial NADPH production (yeast) 5 3 1
sphingosine and sphingosine-1-phosphate metabolism 10 4 2
octane oxidation 5 2 1
L-methionine salvage cycle III 11 5 2
L-methionine salvage cycle II (plants) 11 4 2
fatty acid salvage 6 6 1
stearate biosynthesis II (bacteria and plants) 6 5 1
γ-glutamyl cycle 6 4 1
stearate biosynthesis IV 6 4 1
L-methionine salvage cycle I (bacteria and plants) 12 6 2
NAD(P)/NADPH interconversion 6 3 1
Fe(II) oxidation 6 3 1
6-gingerol analog biosynthesis (engineered) 6 2 1
stearate biosynthesis I (animals) 6 1 1
stachyose degradation 7 3 1
ceramide degradation by α-oxidation 7 2 1
capsaicin biosynthesis 7 1 1
icosapentaenoate biosynthesis II (6-desaturase, mammals) 7 1 1
arachidonate biosynthesis III (6-desaturase, mammals) 7 1 1
icosapentaenoate biosynthesis III (8-desaturase, mammals) 7 1 1
L-citrulline biosynthesis 8 7 1
superpathway of NAD/NADP - NADH/NADPH interconversion (yeast) 8 5 1
2-deoxy-D-ribose degradation II 8 4 1
ceramide and sphingolipid recycling and degradation (yeast) 16 4 2
suberin monomers biosynthesis 20 3 2
superpathway of fatty acid biosynthesis II (plant) 43 37 4
superpathway of L-citrulline metabolism 12 9 1
palmitate biosynthesis II (type II fatty acid synthase) 31 29 2
cutin biosynthesis 16 2 1
superpathway of fatty acids biosynthesis (E. coli) 53 48 2
palmitate biosynthesis III 29 21 1
oleate β-oxidation 35 32 1