Experiment set5S879 for Rhodanobacter sp. FW510-T8

Survival; Treatment=20250415-M4-SZ1-UF; TreatmentDuration=1week; Outgrowth=R2A_Kan5

Group: survival
Media: + Treatment=20250415-M4-SZ1-UF; TreatmentDuration=1week; Outgrowth=R2A_Kan5
Culturing: rhodanobacter_T8_ML1, tube, Aerobic, at 30 (C)
By: Adams lab on 9/22/25

Specific Phenotypes

For 19 genes in this experiment

For survival Treatment=20250415-M4-SZ1-UF; TreatmentDuration=1week; Outgrowth=R2A_Kan5 in Rhodanobacter sp. FW510-T8

For survival Treatment=20250415-M4-SZ1-UF; TreatmentDuration=1week; Outgrowth=R2A_Kan5 across organisms

SEED Subsystems

Subsystem	#Specific
Thiamin biosynthesis	3
ATP-dependent RNA helicases, bacterial	1
Arginine and Ornithine Degradation	1
DNA Repair Base Excision	1
Formate hydrogenase	1
Glycerol and Glycerol-3-phosphate Uptake and Utilization	1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria	1
MLST	1
Proline, 4-hydroxyproline uptake and utilization	1
Respiratory dehydrogenases 1	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Thiamine metabolism
• Arginine and proline metabolism
• Puromycin biosynthesis
• Glutamate metabolism
• Nucleotide sugars metabolism
• Glycerolipid metabolism
• alpha-Linolenic acid metabolism
• Trinitrotoluene degradation
• Porphyrin and chlorophyll metabolism
• Carotenoid biosynthesis - General
• Alkaloid biosynthesis I
• Insect hormone biosynthesis

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
hydroxymethylpyrimidine salvage	2	2	2
thiamine diphosphate salvage II	5	4	3
4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis I	2	2	1
thiamine diphosphate biosynthesis I (E. coli)	2	2	1
thiamine diphosphate biosynthesis II (Bacillus)	2	2	1
thiamine diphosphate salvage IV (yeast)	7	3	3
superpathway of thiamine diphosphate biosynthesis I	10	8	4
thiamine diphosphate formation from pyrithiamine and oxythiamine (yeast)	8	3	3
superpathway of thiamine diphosphate biosynthesis II	11	10	4
L-arginine degradation I (arginase pathway)	3	3	1
thiazole component of thiamine diphosphate biosynthesis I	6	4	2
thiamine diphosphate salvage V	3	2	1
L-proline degradation I	3	2	1
thiamine diphosphate biosynthesis IV (eukaryotes)	3	1	1
thiamine diphosphate biosynthesis III (Staphylococcus)	3	1	1
glycerol degradation I	3	1	1
thiazole component of thiamine diphosphate biosynthesis II	7	6	2
superpathway of thiamine diphosphate biosynthesis III (eukaryotes)	7	3	2
glycerol and glycerophosphodiester degradation	4	2	1
ethene biosynthesis II (microbes)	4	1	1
adipate degradation	5	4	1
fatty acid salvage	6	5	1
4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis II	8	2	1
oleate β-oxidation	35	32	3
2-methyl-branched fatty acid β-oxidation	14	9	1