Experiment set5S667 for Mycobacterium tuberculosis H37Rv

7H9 with 2,4-Dinitrophenol 0.1503 mM

Group: stress
Media: 7H9 + 2,4-Dinitrophenol (0.1503 mM)
Culturing: MycoTube_ML10, tube, Aerobic, at 37 (C)
By: Kayla Dinshaw on 3/4/25
Media components: 0.5 g/L Ammonium Sulfate, 0.5 g/L L-Glutamic acid, 0.1 g/L Trisodium citrate dihydrate, 0.001 g/L Pyridoxine HCl, 0.0005 g/L biotin, 2.5 g/L Disodium phosphate, 1 g/L Potassium phosphate monobasic, 0.04 g/L Ferric ammonium citrate, 0.05 g/L Magnesium sulfate, 0.0005 g/L Calcium chloride, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L copper (II) chloride dihydrate, 5 g/L bovine serum albumin, 2 g/L D-Glucose, 4.25 g/L Sodium Chloride, 0.5 vol% Tween 80

Specific Phenotypes

For 1 genes in this experiment

For stress 2,4-Dinitrophenol in Mycobacterium tuberculosis H37Rv

For stress 2,4-Dinitrophenol across organisms

SEED Subsystems

Subsystem	#Specific
Isobutyryl-CoA to Propionyl-CoA Module	1
Valine degradation	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Valine, leucine and isoleucine degradation
• beta-Alanine metabolism
• Propanoate metabolism
• Fatty acid elongation in mitochondria
• Fatty acid metabolism
• Geraniol degradation
• Lysine degradation
• Tryptophan metabolism
• alpha-Linolenic acid metabolism
• Benzoate degradation via CoA ligation
• Butanoate metabolism
• Limonene and pinene degradation
• Caprolactam degradation
• Biosynthesis of unsaturated fatty acids
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
benzoyl-CoA biosynthesis	3	3	1
2-methyl-branched fatty acid β-oxidation	14	9	3
adipate biosynthesis	5	4	1
adipate degradation	5	4	1
acrylate degradation I	5	3	1
propanoyl-CoA degradation II	5	3	1
fatty acid β-oxidation II (plant peroxisome)	5	3	1
fatty acid β-oxidation IV (unsaturated, even number)	5	2	1
benzoate biosynthesis III (CoA-dependent, non-β-oxidative)	5	1	1
(8E,10E)-dodeca-8,10-dienol biosynthesis	11	6	2
β-alanine biosynthesis II	6	5	1
methyl ketone biosynthesis (engineered)	6	3	1
fatty acid β-oxidation I (generic)	7	4	1
fatty acid β-oxidation VI (mammalian peroxisome)	7	3	1
benzoyl-CoA degradation I (aerobic)	7	2	1
L-valine degradation I	8	7	1
valproate β-oxidation	9	6	1
benzoate biosynthesis I (CoA-dependent, β-oxidative)	9	3	1
phenylacetate degradation I (aerobic)	9	2	1
superpathway of coenzyme A biosynthesis II (plants)	10	9	1
3-phenylpropanoate degradation	10	3	1
superpathway of phenylethylamine degradation	11	4	1
Spodoptera littoralis pheromone biosynthesis	22	4	2
oleate β-oxidation	35	27	3
(4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase)	13	3	1
superpathway of glyoxylate cycle and fatty acid degradation	14	10	1
docosahexaenoate biosynthesis III (6-desaturase, mammals)	14	3	1
platensimycin biosynthesis	26	7	1