Experiment set5S600 for Mycobacterium tuberculosis H37Rv

Compare to:

cholesterol 100uM carbon source

Group: carbon source
Media: Sautons minimal media with no carbon + cholesterol (100 uM)
Culturing: MycoTube_ML10, tube, Aerobic, at 37 (C)
By: Kayla Dinshaw on 3/1/25
Media components: 0.8 g/L Sodium Chloride, 2.5 g/L Disodium phosphate, 1 g/L Potassium phosphate monobasic, 0.2 g/L L-Asparagine, 0.05 g/L Ferric ammonium citrate, 0.02 g/L Magnesium sulfate, 0.0005 g/L Calcium chloride, 1e-05 g/L Zinc sulfate heptahydrate, 0.2 vol% Ethanol, 0.05 vol% Tyloxapol

Specific Phenotypes

For 7 genes in this experiment

For carbon source cholesterol in Mycobacterium tuberculosis H37Rv

For carbon source cholesterol across organisms

SEED Subsystems

Subsystem #Specific
Fermentations: Mixed acid 1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
acetaldehyde biosynthesis I 1 1 1
long-chain fatty acid activation 1 1 1
ethanol degradation I 2 2 1
pyruvate fermentation to ethanol II 2 2 1
γ-linolenate biosynthesis II (animals) 2 2 1
phytol degradation 4 3 2
linoleate biosynthesis II (animals) 2 1 1
ethanol degradation II 3 3 1
L-leucine degradation III 3 2 1
pyruvate fermentation to ethanol I 3 2 1
L-methionine degradation III 3 2 1
formaldehyde oxidation II (glutathione-dependent) 3 2 1
L-valine degradation II 3 2 1
oleate biosynthesis I (plants) 3 2 1
L-isoleucine degradation II 3 2 1
pyruvate fermentation to ethanol III 3 2 1
3-methyl-branched fatty acid α-oxidation 6 3 2
alkane biosynthesis II 3 1 1
L-phenylalanine degradation III 4 3 1
long chain fatty acid ester synthesis (engineered) 4 2 1
L-tyrosine degradation III 4 2 1
salidroside biosynthesis 4 2 1
phosphatidylcholine acyl editing 4 1 1
wax esters biosynthesis II 4 1 1
cytidine-5'-diphosphate-glycerol biosynthesis 4 1 1
sporopollenin precursors biosynthesis 18 4 4
ethanolamine utilization 5 4 1
(S)-propane-1,2-diol degradation 5 4 1
acetylene degradation (anaerobic) 5 4 1
octane oxidation 5 4 1
pyruvate fermentation to isobutanol (engineered) 5 4 1
protein S-nitrosylation and denitrosylation 5 3 1
sphingosine and sphingosine-1-phosphate metabolism 10 4 2
phenylethanol biosynthesis 5 2 1
stearate biosynthesis II (bacteria and plants) 6 5 1
fatty acid salvage 6 5 1
stearate biosynthesis IV 6 4 1
6-gingerol analog biosynthesis (engineered) 6 2 1
stearate biosynthesis I (animals) 6 1 1
noradrenaline and adrenaline degradation 13 12 2
3-methylbutanol biosynthesis (engineered) 7 6 1
serotonin degradation 7 4 1
arachidonate biosynthesis III (6-desaturase, mammals) 7 2 1
icosapentaenoate biosynthesis II (6-desaturase, mammals) 7 2 1
ceramide degradation by α-oxidation 7 2 1
icosapentaenoate biosynthesis III (8-desaturase, mammals) 7 1 1
capsaicin biosynthesis 7 1 1
mycofactocin biosynthesis 15 13 2
butanol and isobutanol biosynthesis (engineered) 8 4 1
ceramide and sphingolipid recycling and degradation (yeast) 16 4 2
androstenedione degradation I (aerobic) 25 22 3
superpathway of fermentation (Chlamydomonas reinhardtii) 9 5 1
superpathway of testosterone and androsterone degradation 28 22 3
suberin monomers biosynthesis 20 2 2
superpathway of fatty acid biosynthesis II (plant) 43 34 4
superpathway of C1 compounds oxidation to CO2 12 3 1
superpathway of Clostridium acetobutylicum solventogenic fermentation 13 6 1
L-tryptophan degradation V (side chain pathway) 13 1 1
superpathway of cholesterol degradation I (cholesterol oxidase) 42 34 3
palmitate biosynthesis II (type II fatty acid synthase) 31 24 2
superpathway of cholesterol degradation II (cholesterol dehydrogenase) 47 36 3
mixed acid fermentation 16 13 1
cutin biosynthesis 16 1 1
superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation 17 8 1
heterolactic fermentation 18 13 1
superpathway of anaerobic sucrose degradation 19 16 1
hexitol fermentation to lactate, formate, ethanol and acetate 19 16 1
superpathway of N-acetylneuraminate degradation 22 16 1
superpathway of fatty acids biosynthesis (E. coli) 53 39 2
androstenedione degradation II (anaerobic) 27 13 1
palmitate biosynthesis III 29 16 1
oleate β-oxidation 35 27 1
superpathway of cholesterol degradation III (oxidase) 49 23 1