Experiment set5S592 for Mycobacterium tuberculosis H37Rv

7H9 with Pyrazinamide 50 ug/mL; pH 5.5

Group: stress
Media: 7H9 + Pyrazinamide (50 ug/mL), pH=5.5
Culturing: MycoTube_ML10, tube, Aerobic, at 37 (C)
By: Kayla Dinshaw on 3/1/25
Media components: 0.5 g/L Ammonium Sulfate, 0.5 g/L L-Glutamic acid, 0.1 g/L Trisodium citrate dihydrate, 0.001 g/L Pyridoxine HCl, 0.0005 g/L biotin, 2.5 g/L Disodium phosphate, 1 g/L Potassium phosphate monobasic, 0.04 g/L Ferric ammonium citrate, 0.05 g/L Magnesium sulfate, 0.0005 g/L Calcium chloride, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L copper (II) chloride dihydrate, 5 g/L bovine serum albumin, 2 g/L D-Glucose, 4.25 g/L Sodium Chloride, 0.5 vol% Tween 80

Specific Phenotypes

For 1 genes in this experiment

For stress Pyrazinamide in Mycobacterium tuberculosis H37Rv

For stress Pyrazinamide across organisms

SEED Subsystems

Subsystem	#Specific
NAD and NADP cofactor biosynthesis global	1
NAD regulation	1
Redox-dependent regulation of nucleus processes	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Nicotinate and nicotinamide metabolism
• Lysine biosynthesis
• Glutathione metabolism
• Nucleotide sugars metabolism
• Lipopolysaccharide biosynthesis
• Sphingolipid metabolism
• 1,4-Dichlorobenzene degradation
• Caprolactam degradation

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
aldoxime degradation	3	1	1
NAD salvage pathway V (PNC V cycle)	5	4	1
NAD salvage pathway I (PNC VI cycle)	7	6	1
NAD salvage (plants)	11	5	1
superpathway of NAD biosynthesis in eukaryotes	14	9	1