Experiment set5IT090 for Rhodospirillum rubrum S1H

L-Malic Acid acid carbon source with ammonium chloride; no light

Group: anaerboic nitrogen source
Media: MELiSSA_defined_MalicAcid_noNitrogen_phosphatebuffered + Ammonium chloride (35.33 mM), pH=7
Culturing: Rubrum_ML4a, serum bottle, Anaerobic, at 30 (C)
By: Kelly Wetmore; Jordan Baker on 4/22/19

Specific Phenotypes

For 8 genes in this experiment

For anaerboic nitrogen source Ammonium chloride in Rhodospirillum rubrum S1H

For anaerboic nitrogen source Ammonium chloride across organisms

SEED Subsystems

Subsystem	#Specific
Alginate metabolism	1
Lacto-N-Biose I and Galacto-N-Biose Metabolic Pathway	1
Lactose and Galactose Uptake and Utilization	1
Mannose Metabolism	1
N-linked Glycosylation in Bacteria	1
Phosphate metabolism	1
Rhamnose containing glycans	1
Teichoic and lipoteichoic acids biosynthesis	1
linker unit-arabinogalactan synthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Fructose and mannose metabolism
• Nucleotide sugars metabolism
• Galactose metabolism
• gamma-Hexachlorocyclohexane degradation
• N-Glycan biosynthesis
• O-Glycan biosynthesis
• High-mannose type N-glycan biosynthesis
• O-Mannosyl glycan biosynthesis
• Keratan sulfate biosynthesis
• Lipopolysaccharide biosynthesis
• Peptidoglycan biosynthesis
• Glycerolipid metabolism
• Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
• Sphingolipid metabolism
• Glycosphingolipid biosynthesis - lacto and neolacto series
• Glycosphingolipid biosynthesis - globo series
• Glycosphingolipid biosynthesis - ganglio series
• Folate biosynthesis
• Carotenoid biosynthesis - General
• Zeatin biosynthesis
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Flavone and flavonol biosynthesis

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
UDP-N-acetyl-D-galactosamine biosynthesis I	1	1	1
UDP-α-D-galactose biosynthesis	1	1	1
diethylphosphate degradation	2	1	1
UDP-α-D-galactofuranose biosynthesis	2	1	1
β-1,4-D-mannosyl-N-acetyl-D-glucosamine degradation	3	2	1
D-galactose detoxification	3	1	1
GDP-mannose biosynthesis	4	4	1
Salmonella enterica serotype O:54 O antigen biosynthesis	5	2	1
D-galactose degradation I (Leloir pathway)	5	2	1
colanic acid building blocks biosynthesis	11	11	2
superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis	6	4	1
UDP-N-acetyl-D-galactosamine biosynthesis II	7	4	1
stachyose degradation	7	2	1
β-(1,4)-mannan degradation	7	2	1
UDP-sugars interconversion	9	3	1
teichuronic acid biosynthesis (B. subtilis 168)	9	2	1
poly(3-O-β-D-glucopyranosyl-N-acetylgalactosamine 1-phosphate) wall teichoic acid biosynthesis	10	2	1
poly(glycerol phosphate) wall teichoic acid biosynthesis	11	3	1
poly(ribitol phosphate) wall teichoic acid biosynthesis I (B. subtilis)	12	2	1
superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis	14	9	1
poly(ribitol phosphate) wall teichoic acid biosynthesis II (S. aureus)	14	2	1
mycolyl-arabinogalactan-peptidoglycan complex biosynthesis	18	1	1
superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis	33	12	1