Experiment set5IT089 for Pseudomonas fluorescens FW300-N2E2

Compare to:

LB with Nickel (II) chloride hexahydrate 0.0025 M

200 most detrimental genes:

  gene name fitness t score description  
Pf6N2E2_5036 +5.4 40.2 Cobalt-zinc-cadmium resistance protein conserved
Pf6N2E2_4068 +4.2 36.7 Phosphate regulon metal ion transporter containing CBS domains compare
Pf6N2E2_5037 +4.0 4.3 Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase) compare
Pf6N2E2_3192 +3.7 8.6 tRNA:Cm32/Um32 methyltransferase compare
Pf6N2E2_5015 +3.7 17.9 Magnesium and cobalt efflux protein CorC compare
Pf6N2E2_4362 +3.6 9.4 DNA-directed RNA polymerase omega subunit (EC 2.7.7.6) compare
Pf6N2E2_4795 +3.5 6.5 Acyl-phosphate:glycerol-3-phosphate O-acyltransferase PlsY compare
Pf6N2E2_4454 +3.4 18.9 Exopolyphosphatase (EC 3.6.1.11) compare
Pf6N2E2_5272 +2.7 11.5 Dihydroneopterin triphosphate epimerase compare
Pf6N2E2_5274 +2.5 14.7 FolM Alternative dihydrofolate reductase 1 conserved
Pf6N2E2_3152 +2.5 13.2 Magnesium and cobalt efflux protein CorC conserved
Pf6N2E2_2936 +2.5 17.4 Cysteine synthase (EC 2.5.1.47) conserved
Pf6N2E2_3151 +2.2 8.5 Glutamate synthase [NADPH] small chain (EC 1.4.1.13) compare
Pf6N2E2_2022 +1.8 10.8 BarA-associated response regulator UvrY (= GacA = SirA) compare
Pf6N2E2_3606 +1.8 13.0 23S rRNA (guanosine-2'-O-) -methyltransferase rlmB (EC 2.1.1.-) compare
Pf6N2E2_464 +1.8 4.8 Sensory histidine kinase QseC compare
Pf6N2E2_2605 +1.7 7.9 3-oxoacyl-[acyl-carrier-protein] synthase, KASIII (EC 2.3.1.180) compare
Pf6N2E2_463 +1.7 9.3 Transcriptional regulatory protein RstA compare
Pf6N2E2_3663 +1.7 11.5 Glycosyl transferase compare
Pf6N2E2_1774 +1.7 3.1 DNA-binding response regulator, LuxR family, near polyamine transporter compare
Pf6N2E2_4353 +1.7 7.5 Hydrogen peroxide-inducible genes activator compare
Pf6N2E2_2519 +1.6 2.5 Biosynthetic Aromatic amino acid aminotransferase beta (EC 2.6.1.57) compare
Pf6N2E2_2292 +1.6 6.3 Universal stress protein family COG0589 compare
Pf6N2E2_204 +1.6 2.4 hypothetical protein compare
Pf6N2E2_4204 +1.5 2.1 rRNA small subunit 7-methylguanosine (m7G) methyltransferase GidB compare
Pf6N2E2_4139 +1.5 10.1 Integral membrane protein TerC compare
Pf6N2E2_4591 +1.5 7.0 tRNA (guanine46-N7-)-methyltransferase (EC 2.1.1.33) compare
Pf6N2E2_4121 +1.4 4.0 Ribosomal RNA small subunit methyltransferase B (EC 2.1.1.-) compare
Pf6N2E2_5252 +1.4 2.6 FIG00956396: hypothetical protein compare
Pf6N2E2_5510 +1.4 4.0 FIG00955324: hypothetical protein compare
Pf6N2E2_5593 +1.4 6.7 Leucyl-tRNA synthetase compare
Pf6N2E2_4479 +1.4 5.3 FIG001590: Putative conserved exported protein precursor compare
Pf6N2E2_3261 +1.4 6.8 hypothetical protein compare
Pf6N2E2_5609 +1.4 7.0 DNA-binding response regulator ColR compare
Pf6N2E2_3665 +1.3 1.5 serine/threonine protein kinase compare
Pf6N2E2_5243 +1.3 2.2 hypothetical protein compare
Pf6N2E2_5989 +1.3 10.2 Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1) compare
Pf6N2E2_3264 +1.3 4.4 Uncharacterized protein YrbK clustered with lipopolysaccharide transporters compare
Pf6N2E2_5676 +1.3 5.1 Aspartokinase (EC 2.7.2.4) compare
Pf6N2E2_3317 +1.3 9.2 Ornithine cyclodeaminase (EC 4.3.1.12) compare
Pf6N2E2_5720 +1.3 9.2 BarA sensory histidine kinase (= VarS = GacS) compare
Pf6N2E2_2536 +1.3 2.0 Fumarate hydratase class II (EC 4.2.1.2) compare
Pf6N2E2_1156 +1.2 3.0 hypothetical protein compare
Pf6N2E2_5575 +1.2 5.8 Glycerol uptake facilitator protein compare
Pf6N2E2_5574 +1.2 8.1 Glycerol kinase (EC 2.7.1.30) compare
Pf6N2E2_405 +1.2 0.8 hypothetical protein compare
Pf6N2E2_5425 +1.2 1.2 hypothetical protein compare
Pf6N2E2_3493 +1.2 4.0 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100) compare
Pf6N2E2_1442 +1.1 4.5 Transcriptional regulators of sugar metabolism compare
Pf6N2E2_529 +1.1 4.3 FIG00954439: hypothetical protein compare
Pf6N2E2_476 +1.1 2.6 Permease of the drug/metabolite transporter (DMT) superfamily compare
Pf6N2E2_3918 +1.1 6.9 D-cysteine desulfhydrase (EC 4.4.1.15) compare
Pf6N2E2_2384 +1.1 4.7 CmpX compare
Pf6N2E2_733 +1.1 2.5 Peptidyl-prolyl cis-trans isomerase PpiD (EC 5.2.1.8) compare
Pf6N2E2_4601 +1.1 2.8 Hypothetical protein YggS, proline synthase co-transcribed bacterial homolog PROSC compare
Pf6N2E2_4415 +1.1 1.8 Putative FMN hydrolase (EC 3.1.3.-); 5-Amino-6-(5'-phosphoribitylamino)uracil phosphatase compare
Pf6N2E2_4802 +1.1 4.4 Thiosulfate sulfurtransferase GlpE (EC 2.8.1.1) compare
Pf6N2E2_3630 +1.1 4.3 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_1981 +1.1 3.4 FIG051360: Periplasmic protein TonB, links inner and outer membranes compare
Pf6N2E2_5095 +1.1 1.7 Putative inner membrane protein (Fragment) compare
Pf6N2E2_4437 +1.1 5.0 Twin-arginine translocation protein TatB compare
Pf6N2E2_2390 +1.1 2.8 FIG00959101: hypothetical protein compare
Pf6N2E2_3763 +1.0 2.8 hypothetical protein compare
Pf6N2E2_1715 +1.0 3.1 hypothetical protein compare
Pf6N2E2_3206 +1.0 0.9 hypothetical protein compare
Pf6N2E2_5841 +1.0 1.4 GlcG protein compare
Pf6N2E2_1376 +1.0 2.9 probable transmembrane protein compare
Pf6N2E2_4555 +1.0 6.6 Lysophospholipase (EC 3.1.1.5) compare
Pf6N2E2_3762 +1.0 4.7 NADP-dependent malic enzyme (EC 1.1.1.40) compare
Pf6N2E2_4511 +1.0 5.6 glutamine synthetase family protein compare
Pf6N2E2_3379 +1.0 7.3 Lactate-responsive regulator LldR in Enterobacteria, GntR family compare
Pf6N2E2_2526 +1.0 4.5 Similar to phosphoglycolate phosphatase, clustered with ubiquinone biosynthesis SAM-dependent O-methyltransferase compare
Pf6N2E2_2278 +1.0 7.9 Transcription-repair coupling factor compare
Pf6N2E2_4987 +1.0 1.2 MaoC-like domain protein compare
Pf6N2E2_2426 +1.0 5.9 Similar to ribosomal large subunit pseudouridine synthase A compare
Pf6N2E2_4821 +0.9 4.4 Phosphoglycolate phosphatase (EC 3.1.3.18) compare
Pf6N2E2_819 +0.9 2.4 Response regulator compare
Pf6N2E2_4624 +0.9 1.0 Phosphoribosyl-dephospho-CoA transferase (EC 2.7.7.-) compare
Pf6N2E2_3828 +0.9 2.5 tRNA (cytidine(34)-2'-O)-methyltransferase (EC 2.1.1.207) compare
Pf6N2E2_1512 +0.9 2.8 Thiamin biosynthesis lipoprotein ApbE compare
Pf6N2E2_4963 +0.9 1.2 Homoserine dehydrogenase (EC 1.1.1.3) compare
Pf6N2E2_238 +0.9 1.7 hypothetical protein compare
Pf6N2E2_5158 +0.9 3.1 FIG00954153: hypothetical protein compare
Pf6N2E2_2309 +0.9 1.8 hypothetical protein compare
Pf6N2E2_2518 +0.9 2.4 Cyclohexadienyl dehydrogenase (EC 1.3.1.12)(EC 1.3.1.43) / 5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19) compare
Pf6N2E2_4590 +0.9 2.5 Thiazole biosynthesis protein ThiG compare
Pf6N2E2_5030 +0.9 3.9 Transcriptional regulator, AsnC family compare
Pf6N2E2_822 +0.9 4.8 TPR domain protein compare
Pf6N2E2_755 +0.9 6.4 UPF0141 membrane protein YijP possibly required for phosphoethanolamine modification of lipopolysaccharide compare
Pf6N2E2_3219 +0.9 2.7 hypothetical protein compare
Pf6N2E2_3947 +0.9 1.3 Cd(II)/Pb(II)-responsive transcriptional regulator compare
Pf6N2E2_311 +0.9 3.0 hypothetical protein compare
Pf6N2E2_4545 +0.9 1.6 hypothetical protein compare
Pf6N2E2_5819 +0.9 4.7 Protein-N(5)-glutamine methyltransferase PrmB, methylates LSU ribosomal protein L3p compare
Pf6N2E2_3326 +0.9 1.8 hypothetical protein compare
Pf6N2E2_1683 +0.9 2.2 Fe2+-dicitrate sensor, membrane component compare
Pf6N2E2_3478 +0.9 4.9 Adenosine deaminase (EC 3.5.4.4) compare
Pf6N2E2_2459 +0.9 1.6 Organic hydroperoxide resistance protein compare
Pf6N2E2_116 +0.9 3.1 Glycosyltransferase compare
Pf6N2E2_4800 +0.8 6.0 yeaH component of nitrogen-related signalling system yeaH (of yeaGH-ycgB) (from data) compare
Pf6N2E2_3318 +0.8 5.3 putative non-ribosomal peptide synthetase compare
Pf6N2E2_3285 +0.8 2.8 Rod shape-determining protein MreD compare
Pf6N2E2_2219 +0.8 1.5 Cell division trigger factor (EC 5.2.1.8) compare
Pf6N2E2_1009 +0.8 1.8 Inositol transport system permease protein compare
Pf6N2E2_5499 +0.8 1.0 hypothetical protein compare
Pf6N2E2_4678 +0.8 1.2 hypothetical protein compare
Pf6N2E2_1448 +0.8 2.4 hypothetical protein compare
Pf6N2E2_2281 +0.8 0.8 Beta N-acetyl-glucosaminidase (EC 3.2.1.52) compare
Pf6N2E2_2681 +0.8 2.6 3-phosphoglycerate kinase compare
Pf6N2E2_4512 +0.8 5.4 Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19) (from data) compare
Pf6N2E2_2606 +0.8 3.3 hypothetical protein compare
Pf6N2E2_156 +0.8 1.6 Carbon storage regulator compare
Pf6N2E2_4445 +0.8 3.5 TPR domain protein compare
Pf6N2E2_2580 +0.8 2.1 Flagellar biosynthesis protein FliP compare
Pf6N2E2_1617 +0.8 1.0 COG2199: FOG: GGDEF domain compare
Pf6N2E2_4630 +0.8 3.1 Ribosomal RNA small subunit methyltransferase E (EC 2.1.1.-) compare
Pf6N2E2_5595 +0.8 3.0 Electron transport complex protein RnfE compare
Pf6N2E2_3096 +0.8 1.2 Periplasmic protein TonB, links inner and outer membranes compare
Pf6N2E2_250 +0.8 2.9 Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain compare
Pf6N2E2_1821 +0.8 1.4 Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system compare
Pf6N2E2_2223 +0.8 2.1 hypothetical protein compare
Pf6N2E2_2288 +0.8 2.4 FIG00953423: hypothetical protein compare
Pf6N2E2_3319 +0.8 4.7 Peptide synthetase compare
Pf6N2E2_5035 +0.8 5.2 FIG00954854: hypothetical protein compare
Pf6N2E2_2795 +0.8 1.4 putative exported protein compare
Pf6N2E2_4364 +0.8 1.5 Protein YicC compare
Pf6N2E2_4799 +0.8 5.6 ycgB component of nitrogen-related signalling system (of yeaGH-ycgB) (from data) compare
Pf6N2E2_5240 +0.8 3.4 FIG00953775: hypothetical protein compare
Pf6N2E2_659 +0.8 1.9 Transcriptional regulatory protein ompR compare
Pf6N2E2_3085 +0.8 1.8 FIG00954614: hypothetical protein compare
Pf6N2E2_4801 +0.8 5.6 yeaG component of nitrogen-related signalling system (of yeaGH-ycgB) (from data) compare
Pf6N2E2_1502 +0.8 1.0 Two-component system regulatory protein compare
Pf6N2E2_3014 +0.7 1.7 P2-like prophage tail protein X compare
Pf6N2E2_4743 +0.7 0.8 hypothetical protein compare
Pf6N2E2_2910 +0.7 3.1 Carbamate kinase (EC 2.7.2.2) compare
Pf6N2E2_395 +0.7 1.4 Glutathione S-transferase (EC 2.5.1.18) compare
Pf6N2E2_3714 +0.7 2.4 FIG017861: hypothetical protein compare
Pf6N2E2_750 +0.7 2.1 Transcriptional regulator, DeoR family compare
Pf6N2E2_6071 +0.7 2.4 FIG139991: Putative thiamine pyrophosphate-requiring enzyme compare
Pf6N2E2_603 +0.7 2.2 FIG00953934: hypothetical protein compare
Pf6N2E2_2312 +0.7 1.4 Cytochrome c oxidase subunit CcoQ (EC 1.9.3.1) compare
Pf6N2E2_5130 +0.7 1.4 hypothetical protein compare
Pf6N2E2_3240 +0.7 1.1 Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63) compare
Pf6N2E2_4059 +0.7 0.9 DNA-binding protein HU-alpha compare
Pf6N2E2_736 +0.7 1.5 hypothetical protein compare
Pf6N2E2_3715 +0.7 2.2 FIGfam138462: Acyl-CoA synthetase, AMP-(fatty) acid ligase / (3R)-hydroxymyristoyl-[ACP] dehydratase (EC 4.2.1.-) compare
Pf6N2E2_3009 +0.7 1.6 Phage tail tube protein compare
Pf6N2E2_5961 +0.7 3.7 Transcriptional regulator, TetR family compare
Pf6N2E2_1044 +0.7 1.8 hypothetical protein compare
Pf6N2E2_1514 +0.7 2.3 lipoprotein OprI, putative compare
Pf6N2E2_3856 +0.7 4.0 Lysine-arginine-ornithine-binding periplasmic protein precursor (TC 3.A.1.3.1) compare
Pf6N2E2_2871 +0.7 1.4 FIG00954271: hypothetical protein compare
Pf6N2E2_218 +0.7 2.0 hypothetical protein compare
Pf6N2E2_2293 +0.7 2.0 COG0488: ATPase components of ABC transporters with duplicated ATPase domains compare
Pf6N2E2_1859 +0.7 1.9 hypothetical protein compare
Pf6N2E2_1860 +0.7 1.9 Ferredoxin, 2Fe-2S compare
Pf6N2E2_4742 +0.7 1.3 Glutathione S-transferase family protein compare
Pf6N2E2_5131 +0.7 3.0 Methyl-accepting chemotaxis protein I (serine chemoreceptor protein) compare
Pf6N2E2_1046 +0.7 1.6 Enoyl-CoA hydratase (EC 4.2.1.17) compare
Pf6N2E2_3472 +0.7 1.7 Probable short-chain dehydrogenase compare
Pf6N2E2_1749 +0.7 2.1 Cyn operon transcriptional activator compare
Pf6N2E2_4754 +0.7 4.8 Protein rarD compare
Pf6N2E2_5159 +0.7 3.3 glutamyl-Q-tRNA synthetase compare
Pf6N2E2_1501 +0.7 2.0 Bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) (EC 3.6.1.17) compare
Pf6N2E2_969 +0.7 2.5 Putative uncharacterized protein STY4534 (Putative uncharacterized protein) compare
Pf6N2E2_923 +0.7 1.2 Haloacid dehalogenase, type II (EC 3.8.1.2) compare
Pf6N2E2_5862 +0.7 0.7 Lipoprotein, putative compare
Pf6N2E2_1648 +0.7 2.2 Maltose/maltodextrin ABC transporter, permease protein MalG compare
Pf6N2E2_5344 +0.7 2.4 Predicted endonuclease distantly related to archaeal Holliday junction resolvase compare
Pf6N2E2_2640 +0.7 1.3 Permease of the drug/metabolite transporter (DMT) superfamily compare
Pf6N2E2_617 +0.7 1.9 Probable glutathione S-transferase compare
Pf6N2E2_160 +0.7 1.4 hypothetical protein compare
Pf6N2E2_5553 +0.7 3.3 Quinolinate synthetase (EC 2.5.1.72) compare
Pf6N2E2_5681 +0.7 4.5 RhtB family transporter compare
Pf6N2E2_2639 +0.7 2.7 Transcriptional regulator, AraC family compare
Pf6N2E2_2235 +0.6 2.7 Xanthine permease compare
Pf6N2E2_3114 +0.6 3.8 Transcriptional regulator, TetR family compare
Pf6N2E2_2861 +0.6 1.9 hypothetical protein compare
Pf6N2E2_4397 +0.6 2.1 Xanthine phosphoribosyltransferase (EC 2.4.2.22) compare
Pf6N2E2_712 +0.6 1.2 hypothetical protein compare
Pf6N2E2_1584 +0.6 1.4 Transcriptional regulator, MarR family / Acetyltransferase (GNAT) compare
Pf6N2E2_5785 +0.6 1.1 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_1192 +0.6 2.5 Transcriptional regulator compare
Pf6N2E2_1746 +0.6 2.2 Spermidine Putrescine ABC transporter permease component potC (TC_3.A.1.11.1) compare
Pf6N2E2_5137 +0.6 2.0 FIG00956650: hypothetical protein compare
Pf6N2E2_5844 +0.6 2.8 Membrane protein, putative compare
Pf6N2E2_2593 +0.6 2.5 Flagellar M-ring protein FliF compare
Pf6N2E2_1090 +0.6 2.2 Transcriptional regulator for ferulate or vanillate catabolism compare
Pf6N2E2_2984 +0.6 1.3 LysR family transcriptional regulator PA3398 compare
Pf6N2E2_1476 +0.6 2.0 (AF179595) Vco33 compare
Pf6N2E2_1412 +0.6 1.3 hypothetical protein compare
Pf6N2E2_1423 +0.6 2.1 Gamma-aminobutyrate permease compare
Pf6N2E2_3466 +0.6 1.3 FIG000605: protein co-occurring with transport systems (COG1739) compare
Pf6N2E2_51 +0.6 3.1 hypothetical protein compare
Pf6N2E2_5064 +0.6 2.2 Xanthine/uracil/thiamine/ascorbate permease family protein compare
Pf6N2E2_5988 +0.6 1.7 hypothetical protein compare
Pf6N2E2_2591 +0.6 2.8 Flagellar assembly protein FliH compare
Pf6N2E2_1391 +0.6 1.4 DNA-binding response regulator, LuxR family compare
Pf6N2E2_1759 +0.6 1.5 uncharacterized domain 1 compare
Pf6N2E2_6077 +0.6 2.6 Cysteine desulfurase (EC 2.8.1.7) compare


Specific Phenotypes

For 16 genes in this experiment

For stress Nickel (II) chloride hexahydrate in Pseudomonas fluorescens FW300-N2E2

For stress Nickel (II) chloride hexahydrate across organisms