Experiment set5IT086 for Pseudomonas fluorescens FW300-N2E2

Compare to:

LB with dimethyl sulfone 0.25 M

Group: stress
Media: LB + dimethyl sulfone (0.25 M), pH=7
Culturing: pseudo6_N2E2_ML5, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=500 rpm
By: Hans_Hualan on 2/2/2016
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 18 genes in this experiment

For stress dimethyl sulfone in Pseudomonas fluorescens FW300-N2E2

For stress dimethyl sulfone across organisms

SEED Subsystems

Subsystem #Specific
Peptidoglycan Biosynthesis 2
Carboxysome 1
Glycogen metabolism 1
Oxidative stress 1
Oxygen and light sensor PpaA-PpsR 1
Respiratory dehydrogenases 1 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
NADH to cytochrome bo oxidase electron transfer II 2 2 1
NADH to cytochrome bo oxidase electron transfer I 2 2 1
NADH to cytochrome bd oxidase electron transfer II 2 1 1
NADH to cytochrome aa3 oxidase electron transfer 2 1 1
NADH to nitrate electron transfer 2 1 1
nitrate reduction VIIIb (dissimilatory) 2 1 1
NADH to cytochrome bd oxidase electron transfer I 2 1 1
aerobic respiration III (alternative oxidase pathway) 3 3 1
glycogen biosynthesis I (from ADP-D-Glucose) 4 3 1
aerobic respiration II (cytochrome c) (yeast) 4 3 1
aerobic respiration I (cytochrome c) 4 3 1
mitochondrial NADPH production (yeast) 5 4 1
NAD(P)/NADPH interconversion 6 3 1
Fe(II) oxidation 6 3 1
peptidoglycan maturation (meso-diaminopimelate containing) 12 4 2
superpathway of NAD/NADP - NADH/NADPH interconversion (yeast) 8 7 1
starch biosynthesis 10 5 1
peptidoglycan biosynthesis IV (Enterococcus faecium) 17 12 1
peptidoglycan biosynthesis II (staphylococci) 17 12 1