Experiment set5IT085 for Pseudomonas fluorescens FW300-N2C3

Compare to:

L-Leucine nitrogen source

Group: nitrogen source
Media: RCH2_defined_Glucose_noNitrogen + L-Leucine (5 mM), pH=7
Culturing: pseudo5_N2-C3_1_ML2, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=750 rpm
By: Mark on 12/17/2014
Media components: 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 20 mM D-Glucose, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 17 genes in this experiment

For nitrogen source L-Leucine in Pseudomonas fluorescens FW300-N2C3

For nitrogen source L-Leucine across organisms

SEED Subsystems

Subsystem #Specific
ABC transporter branched-chain amino acid (TC 3.A.1.4.1) 3
Isoleucine degradation 3
Leucine Degradation and HMG-CoA Metabolism 3
Valine degradation 3
Alanine biosynthesis 1
Branched-Chain Amino Acid Biosynthesis 1
Homogentisate pathway of aromatic compound degradation 1
L-rhamnose utilization 1
Leucine Biosynthesis 1
NAD and NADP cofactor biosynthesis global 1
NAD regulation 1
Orphan regulatory proteins 1
Pyruvate Alanine Serine Interconversions 1
Rhamnose containing glycans 1
Teichoic and lipoteichoic acids biosynthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-alanine biosynthesis I 2 2 1
2-oxoisovalerate decarboxylation to isobutanoyl-CoA 3 3 1
L-valine degradation II 3 2 1
L-isoleucine degradation II 3 2 1
L-isoleucine biosynthesis V 3 2 1
L-leucine degradation III 3 2 1
L-leucine degradation V (oxidative Stickland reaction) 3 1 1
L-valine degradation III (oxidative Stickland reaction) 3 1 1
L-isoleucine degradation III (oxidative Stickland reaction) 3 1 1
superpathway of L-alanine biosynthesis 4 4 1
L-valine biosynthesis 4 4 1
NAD salvage pathway II (PNC IV cycle) 5 3 1
L-leucine degradation IV (reductive Stickland reaction) 5 1 1
superpathway of branched chain amino acid biosynthesis 17 17 3
L-leucine biosynthesis 6 6 1
L-isoleucine degradation I 6 5 1
L-leucine degradation I 6 5 1
NAD de novo biosynthesis I 6 5 1
NAD de novo biosynthesis IV (anaerobic) 6 5 1
NAD de novo biosynthesis III 6 4 1
L-isoleucine biosynthesis IV 6 4 1
L-isoleucine biosynthesis I (from threonine) 7 7 1
L-isoleucine biosynthesis III 7 5 1
NAD salvage pathway I (PNC VI cycle) 7 5 1
L-valine degradation I 8 6 1
L-isoleucine biosynthesis II 8 5 1
Escherichia coli serotype O:8 O antigen biosynthesis 9 2 1
superpathway of L-isoleucine biosynthesis I 13 13 1
superpathway of L-threonine metabolism 18 13 1
aspartate superpathway 25 22 1
odd iso-branched-chain fatty acid biosynthesis 34 30 1
even iso-branched-chain fatty acid biosynthesis 34 30 1
anteiso-branched-chain fatty acid biosynthesis 34 30 1