Experiment set5IT081 for Rhodanobacter denitrificans FW104-10B01

Compare to:

Potassium acetate 20 mM

Group: carbon source
Media: Hans_Basal_Media_plus_0.2x_20AA_mix + Potassium acetate (20 mM)
Culturing: rhodanobacter_10B01_ML12, 96 deep-well microplate; 0.8 mL volume, Aerobic, at 30 (C), shaken=200 rpm
By: Hans Carlson and Trenton Owens on 24-Aug-21
Media components: 0.03 M PIPES sesquisodium salt, 0.1 g/L Potassium Chloride, 0.01 g/L Sodium Chloride, 0.01 g/L Calcium chloride dihydrate, 0.1 g/L Magnesium chloride hexahydrate, 0.1 g/L Sodium sulfate, 0.25 g/L Ammonium chloride, 0.1 g/L Disodium phosphate, 20AA_mix (0.1 mM L-Arginine, 0.1 mM L-Histidine, 0.1 mM L-Lysine, 0.1 mM L-Aspartic Acid, 0.1 mM L-Glutamic acid monopotassium salt monohydrate, 0.1 mM L-Serine, 0.1 mM L-Threonine, 0.1 mM L-Asparagine, 0.1 mM L-Glutamine, 0.1 mM L-Cysteine hydrochloride monohydrate, 0.1 mM Glycine, 0.1 mM L-Proline, 0.1 mM L-Alanine, 0.1 mM L-Valine, 0.1 mM L-Isoleucine, 0.1 mM L-Leucine, 0.1 mM L-Methionine, 0.1 mM L-Phenylalanine, 0.1 mM L-tyrosine disodium salt, 0.1 mM L-Tryptophan), DL vitamins (0.0002 mg/L biotin, 0.0002 mg/L Folic Acid, 0.001 mg/L Pyridoxine HCl, 0.0005 mg/L Riboflavin, 0.0005 mg/L Thiamine HCl, 0.0005 mg/L Nicotinic Acid, 0.0005 mg/L calcium pantothenate, 1e-05 mg/L Cyanocobalamin, 0.0005 mg/L 4-Aminobenzoic acid, 0.0005 mg/L Lipoic acid), Sulfur-free DL minerals (0.0003 g/L Magnesium chloride hexahydrate, 0.00015 g/L Nitrilotriacetic acid disodium salt, 0.0001 g/L Sodium Chloride, 5e-05 g/L Manganese (II) chloride tetrahydrate, 1e-05 g/L Cobalt chloride hexahydrate, 1.3e-05 g/L Zinc chloride, 1e-05 g/L Calcium chloride dihydrate, 1e-05 g/L Iron (II) chloride tetrahydrate, 2.5e-06 g/L Nickel (II) chloride hexahydrate, 2e-06 g/L Aluminum chloride hydrate, 1e-06 g/L copper (II) chloride dihydrate, 1e-06 g/L Boric Acid, 1e-06 g/L Sodium Molybdate Dihydrate, 3e-05 g/L Sodium selenite pentahydrate, 2.5e-05 g/L Sodium tungstate dihydrate)
Growth plate: 2 E3

Specific Phenotypes

For 19 genes in this experiment

For carbon source Potassium acetate in Rhodanobacter denitrificans FW104-10B01

For carbon source Potassium acetate across organisms

SEED Subsystems

Subsystem #Specific
Cysteine Biosynthesis 2
Photorespiration (oxidative C2 cycle) 2
Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate 2
Acetyl-CoA fermentation to Butyrate 1
Butanol Biosynthesis 1
Coenzyme B12 biosynthesis 1
DNA-binding regulatory proteins, strays 1
Dissimilatory nitrite reductase 1
Entner-Doudoroff Pathway 1
Experimental tye 1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria 1
Glycine and Serine Utilization 1
Glycine cleavage system 1
Heme and Siroheme Biosynthesis 1
Isoleucine degradation 1
Ketoisovalerate oxidoreductase 1
Methylglyoxal Metabolism 1
Oxidative stress 1
Polyhydroxybutyrate metabolism 1
Protein chaperones 1
Proteolysis in bacteria, ATP-dependent 1
Serine-glyoxylate cycle 1
Thioredoxin-disulfide reductase 1
Valine degradation 1
n-Phenylalkanoic acid degradation 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
siroheme biosynthesis 4 4 4
acetate and ATP formation from acetyl-CoA III 1 1 1
acetate conversion to acetyl-CoA 1 1 1
fatty acid β-oxidation III (unsaturated, odd number) 1 1 1
ethanol degradation II 3 3 2
ethanol degradation IV 3 3 2
assimilatory sulfate reduction III 3 3 2
benzoyl-CoA biosynthesis 3 3 2
ethanol degradation III 3 2 2
assimilatory sulfate reduction I 4 4 2
glycolate and glyoxylate degradation II 2 2 1
chitin deacetylation 4 2 2
putrescine degradation V 2 1 1
putrescine degradation I 2 1 1
ethylene glycol degradation 2 1 1
oleate β-oxidation (thioesterase-dependent, yeast) 2 1 1
oleate β-oxidation 35 32 16
fatty acid β-oxidation I (generic) 7 6 3
factor 430 biosynthesis 7 3 3
adipate degradation 5 5 2
adipate biosynthesis 5 4 2
fatty acid β-oxidation IV (unsaturated, even number) 5 3 2
fatty acid β-oxidation II (plant peroxisome) 5 3 2
glutaryl-CoA degradation 5 3 2
fatty acid β-oxidation V (unsaturated, odd number, di-isomerase-dependent) 5 2 2
pyruvate fermentation to hexanol (engineered) 11 7 4
(8E,10E)-dodeca-8,10-dienol biosynthesis 11 5 4
2-methyl-branched fatty acid β-oxidation 14 9 5
glyoxylate cycle 6 6 2
glycine cleavage 3 3 1
glycine biosynthesis II 3 3 1
superpathway of acetate utilization and formation 3 3 1
fatty acid salvage 6 5 2
L-isoleucine degradation I 6 4 2
pyruvate fermentation to butanol II (engineered) 6 4 2
hypotaurine degradation 3 2 1
putrescine degradation IV 3 2 1
L-isoleucine biosynthesis V 3 2 1
valproate β-oxidation 9 5 3
propanoate fermentation to 2-methylbutanoate 6 3 2
methyl ketone biosynthesis (engineered) 6 3 2
oleate β-oxidation (reductase-dependent, yeast) 3 1 1
histamine degradation 3 1 1
superpathway of glyoxylate cycle and fatty acid degradation 14 11 4
superpathway of glycol metabolism and degradation 7 4 2
pyruvate fermentation to butanoate 7 3 2
benzoyl-CoA degradation I (aerobic) 7 3 2
fatty acid β-oxidation VI (mammalian peroxisome) 7 3 2
assimilatory sulfate reduction IV 4 3 1
phytol degradation 4 3 1
fatty acid α-oxidation I (plants) 4 2 1
L-valine degradation I 8 3 2
pyruvate fermentation to butanol I 8 3 2
4-methylphenol degradation to protocatechuate 4 1 1
D-arabinose degradation II 4 1 1
oleate β-oxidation (isomerase-dependent, yeast) 4 1 1
putrescine degradation III 4 1 1
L-tryptophan degradation X (mammalian, via tryptamine) 4 1 1
superpathway of sulfate assimilation and cysteine biosynthesis 9 8 2
superpathway of Clostridium acetobutylicum acidogenic fermentation 9 5 2
phenylacetate degradation I (aerobic) 9 3 2
benzoate biosynthesis I (CoA-dependent, β-oxidative) 9 3 2
superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) 10 8 2
2-methylcitrate cycle I 5 4 1
L-glutamate degradation V (via hydroxyglutarate) 10 6 2
(R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) 5 3 1
3-phenylpropanoate degradation 10 5 2
sphingosine and sphingosine-1-phosphate metabolism 10 4 2
9-cis, 11-trans-octadecadienoyl-CoA degradation (isomerase-dependent, yeast) 10 4 2
mitochondrial NADPH production (yeast) 5 2 1
4-hydroxybenzoate biosynthesis III (plants) 5 2 1
octane oxidation 5 2 1
cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion) 15 3 3
benzoate biosynthesis III (CoA-dependent, non-β-oxidative) 5 1 1
dopamine degradation 5 1 1
superpathway of phenylethylamine degradation 11 3 2
superpathway of L-methionine biosynthesis (by sulfhydrylation) 12 11 2
superpathway of glyoxylate bypass and TCA 12 10 2
L-isoleucine biosynthesis IV 6 4 1
3-methyl-branched fatty acid α-oxidation 6 3 1
2-methylcitrate cycle II 6 3 1
L-glutamate degradation VII (to butanoate) 12 4 2
6-gingerol analog biosynthesis (engineered) 6 2 1
superpathway of bitter acids biosynthesis 18 3 3
lupulone and humulone biosynthesis 6 1 1
β-alanine biosynthesis II 6 1 1
colupulone and cohumulone biosynthesis 6 1 1
4-hydroxymandelate degradation 6 1 1
adlupulone and adhumulone biosynthesis 6 1 1
alkane oxidation 6 1 1
noradrenaline and adrenaline degradation 13 4 2
superpathway of Clostridium acetobutylicum solventogenic fermentation 13 4 2
cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation) 13 2 2
serotonin degradation 7 3 1
ceramide degradation by α-oxidation 7 2 1
2,4-xylenol degradation to protocatechuate 7 1 1
limonene degradation IV (anaerobic) 7 1 1
D-xylose degradation IV 7 1 1
Spodoptera littoralis pheromone biosynthesis 22 3 3
L-tryptophan degradation III (eukaryotic) 15 11 2
glycerol degradation to butanol 16 9 2
superpathway of NAD/NADP - NADH/NADPH interconversion (yeast) 8 4 1
ceramide and sphingolipid recycling and degradation (yeast) 16 4 2
L-arabinose degradation IV 8 2 1
2-methylpropene degradation 8 2 1
superpathway of ornithine degradation 8 2 1
crotonate fermentation (to acetate and cyclohexane carboxylate) 16 3 2
aromatic biogenic amine degradation (bacteria) 8 1 1
superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation 17 6 2
benzoate fermentation (to acetate and cyclohexane carboxylate) 17 3 2
3-hydroxypropanoate/4-hydroxybutanate cycle 18 10 2
reductive glycine pathway of autotrophic CO2 fixation 9 5 1
Entner-Doudoroff pathway II (non-phosphorylative) 9 4 1
cis-geranyl-CoA degradation 9 2 1
toluene degradation VI (anaerobic) 18 3 2
superpathway of coenzyme A biosynthesis II (plants) 10 5 1
methyl tert-butyl ether degradation 10 3 1
toluene degradation III (aerobic) (via p-cresol) 11 5 1
superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation 11 3 1
gallate degradation III (anaerobic) 11 3 1
adenosylcobalamin biosynthesis I (anaerobic) 36 12 3
10-trans-heptadecenoyl-CoA degradation (reductase-dependent, yeast) 12 2 1
10-cis-heptadecenoyl-CoA degradation (yeast) 12 2 1
androstenedione degradation I (aerobic) 25 8 2
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass 26 23 2
superpathway of L-arginine and L-ornithine degradation 13 4 1
platensimycin biosynthesis 26 6 2
(4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) 13 2 1
1-butanol autotrophic biosynthesis (engineered) 27 18 2
androstenedione degradation II (anaerobic) 27 6 2
superpathway of testosterone and androsterone degradation 28 8 2
superpathway of cholesterol degradation I (cholesterol oxidase) 42 10 3
crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) 14 2 1
docosahexaenoate biosynthesis III (6-desaturase, mammals) 14 2 1
superpathway of cholesterol degradation II (cholesterol dehydrogenase) 47 10 3
adenosylcobalamin biosynthesis II (aerobic) 33 12 2
cholesterol degradation to androstenedione I (cholesterol oxidase) 17 2 1
superpathway of pentose and pentitol degradation 42 4 2
cholesterol degradation to androstenedione II (cholesterol dehydrogenase) 22 2 1
superpathway of cholesterol degradation III (oxidase) 49 6 2
photosynthetic 3-hydroxybutanoate biosynthesis (engineered) 26 17 1
superpathway of aerobic toluene degradation 30 7 1
superpathway of aromatic compound degradation via 3-oxoadipate 35 11 1
superpathway of aromatic compound degradation via 2-hydroxypentadienoate 42 8 1