Experiment set5IT078 for Pseudomonas fluorescens FW300-N2C3

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D-Glucosamine Hydrochloride carbon source

Group: carbon source
Media: RCH2_defined_noCarbon + D-Glucosamine Hydrochloride (20 mM), pH=7
Culturing: pseudo5_N2-C3_1_ML2, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=750 rpm
By: Mark on 12/17/2014
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 12 genes in this experiment

For carbon source D-Glucosamine Hydrochloride in Pseudomonas fluorescens FW300-N2C3

For carbon source D-Glucosamine Hydrochloride across organisms

SEED Subsystems

Subsystem #Specific
Alginate metabolism 1
Arsenic resistance 1
Chorismate Synthesis 1
Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate) 1
D-gluconate and ketogluconates metabolism 1
Mannose Metabolism 1
Rhamnose containing glycans 1
Teichoic and lipoteichoic acids biosynthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
D-serine degradation 3 3 3
L-cysteine degradation II 3 3 2
L-serine degradation 3 3 2
L-tryptophan degradation II (via pyruvate) 3 2 2
D-mannose degradation II 2 1 1
D-mannose degradation I 2 1 1
felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis 5 2 2
L-methionine biosynthesis II 6 5 2
β-1,4-D-mannosyl-N-acetyl-D-glucosamine degradation 3 2 1
mannitol biosynthesis 3 1 1
glycine betaine degradation III 7 7 2
GDP-mannose biosynthesis 4 4 1
glycine betaine degradation I 8 6 2
L-mimosine degradation 8 4 2
mannitol degradation II 4 2 1
glutathione-mediated detoxification I 8 3 2
chorismate biosynthesis from 3-dehydroquinate 5 5 1
1,5-anhydrofructose degradation 5 2 1
chorismate biosynthesis I 7 7 1
β-(1,4)-mannan degradation 7 2 1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II 15 13 2
Escherichia coli serotype O:8 O antigen biosynthesis 9 2 1
superpathway of L-phenylalanine biosynthesis 10 10 1
superpathway of L-tyrosine biosynthesis 10 10 1
colanic acid building blocks biosynthesis 11 9 1
purine nucleobases degradation II (anaerobic) 24 16 2
chorismate biosynthesis II (archaea) 12 8 1
superpathway of L-tryptophan biosynthesis 13 13 1
superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis 14 7 1
superpathway of aromatic amino acid biosynthesis 18 18 1
superpathway of chorismate metabolism 59 44 1