Experiment set5IT077 for Pseudomonas fluorescens FW300-N2C3

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N-Acetyl-D-Glucosamine carbon source

Group: carbon source
Media: RCH2_defined_noCarbon + N-Acetyl-D-Glucosamine (20 mM), pH=7
Culturing: pseudo5_N2-C3_1_ML2, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=750 rpm
By: Mark on 12/17/2014
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 18 genes in this experiment

For carbon source N-Acetyl-D-Glucosamine in Pseudomonas fluorescens FW300-N2C3

For carbon source N-Acetyl-D-Glucosamine across organisms

SEED Subsystems

Subsystem #Specific
Chitin and N-acetylglucosamine utilization 2
Sialic Acid Metabolism 2
Glycolysis and Gluconeogenesis 1
Glycolysis and Gluconeogenesis, including Archaeal enzymes 1
Murein hydrolase regulation and cell death 1
Pyruvate metabolism I: anaplerotic reactions, PEP 1
Rhamnose containing glycans 1
Teichoic and lipoteichoic acids biosynthesis 1
UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
N-acetylglucosamine degradation I 2 2 1
N-acetylglucosamine degradation II 3 2 1
D-galactosamine and N-acetyl-D-galactosamine degradation 4 2 1
N-acetyl-D-galactosamine degradation 5 2 1
superpathway of N-acetylglucosamine, N-acetylmannosamine and N-acetylneuraminate degradation 6 2 1
chitin derivatives degradation 8 2 1
Escherichia coli serotype O:8 O antigen biosynthesis 9 2 1
glycolysis V (Pyrococcus) 10 7 1
glycolysis II (from fructose 6-phosphate) 11 9 1
superpathway of N-acetylneuraminate degradation 22 15 2
gluconeogenesis I 13 11 1
glycolysis I (from glucose 6-phosphate) 13 10 1
peptidoglycan recycling I 14 11 1
superpathway of glycolysis and the Entner-Doudoroff pathway 17 14 1
superpathway of hexitol degradation (bacteria) 18 12 1
gluconeogenesis II (Methanobacterium thermoautotrophicum) 18 9 1
superpathway of anaerobic sucrose degradation 19 15 1
hexitol fermentation to lactate, formate, ethanol and acetate 19 15 1
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass 26 21 1
Methanobacterium thermoautotrophicum biosynthetic metabolism 56 21 1