L-Glutamic acid monopotassium salt monohydrate 20 mM
Group:
carbon source
Media:
Hans_Basal_Media_plus_0.2x_20AA_mix +
L-Glutamic acid monopotassium salt monohydrate (20 mM)
Culturing: rhodanobacter_10B01_ML12, 96 deep-well microplate; 0.8 mL volume, Aerobic, at 30 (C), shaken=700 rpm
By: Hans Carlson and Trenton Owens on
08/24/2021
Media components: 0.03 M
PIPES sesquisodium salt, 0.1 g/L
Potassium Chloride, 0.01 g/L
Sodium Chloride, 0.01 g/L
Calcium chloride dihydrate, 0.1 g/L
Magnesium chloride hexahydrate, 0.1 g/L
Sodium sulfate, 0.25 g/L
Ammonium chloride, 0.1 g/L
Disodium phosphate, 20AA_mix
(0.1 mM L-Arginine, 0.1 mM L-Histidine, 0.1 mM L-Lysine, 0.1 mM L-Aspartic Acid, 0.1 mM L-Glutamic acid monopotassium salt monohydrate, 0.1 mM L-Serine, 0.1 mM L-Threonine, 0.1 mM L-Asparagine, 0.1 mM L-Glutamine, 0.1 mM L-Cysteine hydrochloride monohydrate, 0.1 mM Glycine, 0.1 mM L-Proline, 0.1 mM L-Alanine, 0.1 mM L-Valine, 0.1 mM L-Isoleucine, 0.1 mM L-Leucine, 0.1 mM L-Methionine, 0.1 mM L-Phenylalanine, 0.1 mM L-tyrosine disodium salt, 0.1 mM L-Tryptophan), DL vitamins
(0.0002 mg/L biotin, 0.0002 mg/L Folic Acid, 0.001 mg/L Pyridoxine HCl, 0.0005 mg/L Riboflavin, 0.0005 mg/L Thiamine HCl, 0.0005 mg/L Nicotinic Acid, 0.0005 mg/L calcium pantothenate, 1e-05 mg/L Cyanocobalamin, 0.0005 mg/L 4-Aminobenzoic acid, 0.0005 mg/L Lipoic acid), Sulfur-free DL minerals
(0.0003 g/L Magnesium chloride hexahydrate, 0.00015 g/L Nitrilotriacetic acid disodium salt, 0.0001 g/L Sodium Chloride, 5e-05 g/L Manganese (II) chloride tetrahydrate, 1e-05 g/L Cobalt chloride hexahydrate, 1.3e-05 g/L Zinc chloride, 1e-05 g/L Calcium chloride dihydrate, 1e-05 g/L Iron (II) chloride tetrahydrate, 2.5e-06 g/L Nickel (II) chloride hexahydrate, 2e-06 g/L Aluminum chloride hydrate, 1e-06 g/L copper (II) chloride dihydrate, 1e-06 g/L Boric Acid, 1e-06 g/L Sodium Molybdate Dihydrate, 3e-05 g/L Sodium selenite pentahydrate, 2.5e-05 g/L Sodium tungstate dihydrate)
Growth plate: 2 E7
Specific Phenotypes
For 3 genes in this experiment
For carbon source L-Glutamic acid monopotassium salt monohydrate in Rhodanobacter denitrificans FW104-10B01
For carbon source L-Glutamic acid monopotassium salt monohydrate across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|
| acetate and ATP formation from acetyl-CoA I | 2 | 2 | 1 |
| tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate | 2 | 2 | 1 |
| glycine degradation (reductive Stickland reaction) | 2 | 1 | 1 |
| superpathway of 5-aminoimidazole ribonucleotide biosynthesis | 6 | 6 | 2 |
| superpathway of acetate utilization and formation | 3 | 3 | 1 |
| pyruvate fermentation to acetate II | 3 | 3 | 1 |
| pyruvate fermentation to acetate I | 3 | 2 | 1 |
| pyruvate fermentation to acetate VII | 3 | 2 | 1 |
| pyruvate fermentation to acetate IV | 3 | 2 | 1 |
| L-methionine salvage from L-homocysteine | 3 | 1 | 1 |
| L-methionine biosynthesis III | 4 | 3 | 1 |
| pyruvate fermentation to acetate and (S)-lactate I | 4 | 3 | 1 |
| pyruvate fermentation to acetate and lactate II | 4 | 2 | 1 |
| 5-aminoimidazole ribonucleotide biosynthesis I | 5 | 5 | 1 |
| 5-aminoimidazole ribonucleotide biosynthesis II | 5 | 5 | 1 |
| ethanolamine utilization | 5 | 3 | 1 |
| acetylene degradation (anaerobic) | 5 | 3 | 1 |
| L-methionine biosynthesis I | 5 | 3 | 1 |
| methanogenesis from acetate | 6 | 2 | 1 |
| acetyl-CoA fermentation to butanoate | 7 | 5 | 1 |
| superpathway of L-homoserine and L-methionine biosynthesis | 8 | 6 | 1 |
| lactate fermentation to acetate, CO2 and hydrogen (Desulfovibrionales) | 8 | 2 | 1 |
| folate transformations III (E. coli) | 9 | 9 | 1 |
| superpathway of S-adenosyl-L-methionine biosynthesis | 9 | 7 | 1 |
| superpathway of L-methionine biosynthesis (transsulfuration) | 9 | 7 | 1 |
| superpathway of Clostridium acetobutylicum acidogenic fermentation | 9 | 5 | 1 |
| superpathway of fermentation (Chlamydomonas reinhardtii) | 9 | 5 | 1 |
| reductive glycine pathway of autotrophic CO2 fixation | 9 | 5 | 1 |
| superpathway of L-alanine fermentation (Stickland reaction) | 9 | 4 | 1 |
| hexitol fermentation to lactate, formate, ethanol and acetate | 19 | 14 | 2 |
| glycolysis V (Pyrococcus) | 10 | 7 | 1 |
| L-lysine fermentation to acetate and butanoate | 10 | 3 | 1 |
| glycolysis II (from fructose 6-phosphate) | 11 | 11 | 1 |
| folate transformations II (plants) | 11 | 10 | 1 |
| superpathway of N-acetylneuraminate degradation | 22 | 16 | 2 |
| gallate degradation III (anaerobic) | 11 | 3 | 1 |
| superpathway of L-methionine biosynthesis (by sulfhydrylation) | 12 | 11 | 1 |
| superpathway of tetrahydrofolate biosynthesis and salvage | 12 | 10 | 1 |
| glycolysis I (from glucose 6-phosphate) | 13 | 12 | 1 |
| gluconeogenesis I | 13 | 11 | 1 |
| folate transformations I | 13 | 8 | 1 |
| (S)-lactate fermentation to propanoate, acetate and hydrogen | 13 | 5 | 1 |
| Bifidobacterium shunt | 15 | 14 | 1 |
| purine nucleobases degradation I (anaerobic) | 15 | 3 | 1 |
| mixed acid fermentation | 16 | 12 | 1 |
| superpathway of L-methionine salvage and degradation | 16 | 8 | 1 |
| superpathway of glycolysis and the Entner-Doudoroff pathway | 17 | 16 | 1 |
| superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation | 17 | 6 | 1 |
| superpathway of L-lysine, L-threonine and L-methionine biosynthesis I | 18 | 16 | 1 |
| superpathway of hexitol degradation (bacteria) | 18 | 14 | 1 |
| gluconeogenesis II (Methanobacterium thermoautotrophicum) | 18 | 8 | 1 |
| superpathway of anaerobic sucrose degradation | 19 | 16 | 1 |
| superpathway of purine nucleotides de novo biosynthesis I | 21 | 21 | 1 |
| superpathway of methanogenesis | 21 | 2 | 1 |
| purine nucleobases degradation II (anaerobic) | 24 | 9 | 1 |
| aspartate superpathway | 25 | 23 | 1 |
| superpathway of purine nucleotides de novo biosynthesis II | 26 | 24 | 1 |
| superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass | 26 | 23 | 1 |
| superpathway of L-lysine degradation | 43 | 7 | 1 |
| superpathway of histidine, purine, and pyrimidine biosynthesis | 46 | 44 | 1 |
| Methanobacterium thermoautotrophicum biosynthetic metabolism | 56 | 25 | 1 |