Culturing: pseudo5_N2-C3_1_ML2, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=750 rpm
| Pathway | #Steps | #Present | #Specific |
|---|
| L-carnitine degradation III | 3 | 3 | 3 |
| glycine biosynthesis I | 1 | 1 | 1 |
| ketolysis | 3 | 3 | 2 |
| benzoyl-CoA biosynthesis | 3 | 3 | 2 |
| D-carnitine degradation I | 3 | 2 | 2 |
| glycine betaine degradation III | 7 | 7 | 4 |
| acetate and ATP formation from acetyl-CoA I | 2 | 2 | 1 |
| sulfoacetaldehyde degradation I | 2 | 1 | 1 |
| acetoacetate degradation (to acetyl CoA) | 2 | 1 | 1 |
| D-carnitine degradation II | 2 | 1 | 1 |
| oleate β-oxidation | 35 | 30 | 16 |
| valproate β-oxidation | 9 | 7 | 4 |
| 4-hydroxybenzoate biosynthesis III (plants) | 5 | 4 | 2 |
| (R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) | 5 | 4 | 2 |
| 5,6-dehydrokavain biosynthesis (engineered) | 10 | 7 | 4 |
| fatty acid β-oxidation II (plant peroxisome) | 5 | 3 | 2 |
| glutaryl-CoA degradation | 5 | 3 | 2 |
| glycine betaine degradation I | 8 | 6 | 3 |
| pyruvate fermentation to hexanol (engineered) | 11 | 7 | 4 |
| 2-methyl-branched fatty acid β-oxidation | 14 | 11 | 5 |
| fatty acid salvage | 6 | 6 | 2 |
| pyruvate fermentation to acetate II | 3 | 3 | 1 |
| glycine degradation | 3 | 3 | 1 |
| dTMP de novo biosynthesis (mitochondrial) | 3 | 3 | 1 |
| superpathway of acetate utilization and formation | 3 | 3 | 1 |
| pyruvate fermentation to butanol II (engineered) | 6 | 4 | 2 |
| polyhydroxybutanoate biosynthesis | 3 | 2 | 1 |
| pyruvate fermentation to acetate VII | 3 | 2 | 1 |
| pyruvate fermentation to acetate I | 3 | 2 | 1 |
| pyruvate fermentation to acetate IV | 3 | 2 | 1 |
| superpathway of Clostridium acetobutylicum acidogenic fermentation | 9 | 5 | 3 |
| fatty acid β-oxidation I (generic) | 7 | 5 | 2 |
| fatty acid β-oxidation VI (mammalian peroxisome) | 7 | 4 | 2 |
| pyruvate fermentation to butanoate | 7 | 3 | 2 |
| acetyl-CoA fermentation to butanoate | 7 | 3 | 2 |
| superpathway of L-serine and glycine biosynthesis I | 4 | 4 | 1 |
| pyruvate fermentation to acetate and (S)-lactate I | 4 | 4 | 1 |
| pyruvate fermentation to acetate and lactate II | 4 | 3 | 1 |
| 2-deoxy-D-ribose degradation II | 8 | 4 | 2 |
| creatinine degradation I | 4 | 2 | 1 |
| pyruvate fermentation to butanol I | 8 | 3 | 2 |
| 2-methylpropene degradation | 8 | 2 | 2 |
| sulfolactate degradation II | 4 | 1 | 1 |
| (2S)-ethylmalonyl-CoA biosynthesis | 4 | 1 | 1 |
| glycine betaine degradation II (mammalian) | 4 | 1 | 1 |
| benzoate biosynthesis I (CoA-dependent, β-oxidative) | 9 | 4 | 2 |
| ethanolamine utilization | 5 | 5 | 1 |
| adipate degradation | 5 | 5 | 1 |
| folate polyglutamylation | 5 | 4 | 1 |
| acetylene degradation (anaerobic) | 5 | 4 | 1 |
| adipate biosynthesis | 5 | 4 | 1 |
| L-glutamate degradation V (via hydroxyglutarate) | 10 | 6 | 2 |
| ketogenesis | 5 | 3 | 1 |
| 3-phenylpropanoate degradation | 10 | 5 | 2 |
| 9-cis, 11-trans-octadecadienoyl-CoA degradation (isomerase-dependent, yeast) | 10 | 4 | 2 |
| (S)-propane-1,2-diol degradation | 5 | 2 | 1 |
| fatty acid β-oxidation VII (yeast peroxisome) | 5 | 2 | 1 |
| L-lysine fermentation to acetate and butanoate | 10 | 3 | 2 |
| androstenedione degradation I (aerobic) | 25 | 6 | 5 |
| methyl tert-butyl ether degradation | 10 | 2 | 2 |
| pyruvate fermentation to acetone | 5 | 1 | 1 |
| ethylbenzene degradation (anaerobic) | 5 | 1 | 1 |
| creatinine degradation II | 5 | 1 | 1 |
| isopropanol biosynthesis (engineered) | 5 | 1 | 1 |
| folate transformations II (plants) | 11 | 10 | 2 |
| superpathway of testosterone and androsterone degradation | 28 | 6 | 5 |
| superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation | 17 | 7 | 3 |
| peptido-conjugates in tissue regeneration biosynthesis | 17 | 6 | 3 |
| L-isoleucine degradation I | 6 | 5 | 1 |
| propanoate fermentation to 2-methylbutanoate | 6 | 4 | 1 |
| L-threonine degradation I | 6 | 4 | 1 |
| NAD(P)/NADPH interconversion | 6 | 3 | 1 |
| 4-ethylphenol degradation (anaerobic) | 6 | 3 | 1 |
| methyl ketone biosynthesis (engineered) | 6 | 3 | 1 |
| L-glutamate degradation VII (to butanoate) | 12 | 4 | 2 |
| leukotriene biosynthesis | 6 | 2 | 1 |
| superpathway of taurine degradation | 6 | 2 | 1 |
| superpathway of sulfolactate degradation | 6 | 2 | 1 |
| methanogenesis from acetate | 6 | 2 | 1 |
| superpathway of cholesterol degradation I (cholesterol oxidase) | 42 | 9 | 7 |
| 10-trans-heptadecenoyl-CoA degradation (MFE-dependent, yeast) | 6 | 1 | 1 |
| jasmonic acid biosynthesis | 19 | 4 | 3 |
| superpathway of Clostridium acetobutylicum solventogenic fermentation | 13 | 5 | 2 |
| superpathway of cholesterol degradation II (cholesterol dehydrogenase) | 47 | 9 | 7 |
| androstenedione degradation II (anaerobic) | 27 | 4 | 4 |
| superpathway of glyoxylate cycle and fatty acid degradation | 14 | 11 | 2 |
| benzoyl-CoA degradation I (aerobic) | 7 | 3 | 1 |
| mevalonate pathway II (haloarchaea) | 7 | 2 | 1 |
| mevalonate pathway I (eukaryotes and bacteria) | 7 | 2 | 1 |
| L-tryptophan degradation III (eukaryotic) | 15 | 3 | 2 |
| glycerol degradation to butanol | 16 | 9 | 2 |
| lactate fermentation to acetate, CO2 and hydrogen (Desulfovibrionales) | 8 | 3 | 1 |
| crotonate fermentation (to acetate and cyclohexane carboxylate) | 16 | 4 | 2 |
| mevalonate pathway III (Thermoplasma) | 8 | 2 | 1 |
| isoprene biosynthesis II (engineered) | 8 | 2 | 1 |
| mevalonate pathway IV (archaea) | 8 | 2 | 1 |
| benzoate fermentation (to acetate and cyclohexane carboxylate) | 17 | 4 | 2 |
| cholesterol degradation to androstenedione I (cholesterol oxidase) | 17 | 3 | 2 |
| folate transformations III (E. coli) | 9 | 9 | 1 |
| TCA cycle VI (Helicobacter) | 9 | 7 | 1 |
| photorespiration I | 9 | 5 | 1 |
| photorespiration III | 9 | 5 | 1 |
| superpathway of fermentation (Chlamydomonas reinhardtii) | 9 | 5 | 1 |
| 3-hydroxypropanoate/4-hydroxybutanate cycle | 18 | 8 | 2 |
| superpathway of L-alanine fermentation (Stickland reaction) | 9 | 4 | 1 |
| phenylacetate degradation I (aerobic) | 9 | 3 | 1 |
| toluene degradation VI (anaerobic) | 18 | 4 | 2 |
| gliotoxin biosynthesis | 9 | 2 | 1 |
| 4-oxopentanoate degradation | 9 | 1 | 1 |
| photorespiration II | 10 | 6 | 1 |
| superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate) | 10 | 5 | 1 |
| (8E,10E)-dodeca-8,10-dienol biosynthesis | 11 | 6 | 1 |
| superpathway of phenylethylamine degradation | 11 | 5 | 1 |
| gallate degradation III (anaerobic) | 11 | 3 | 1 |
| cholesterol degradation to androstenedione II (cholesterol dehydrogenase) | 22 | 3 | 2 |
| ethylmalonyl-CoA pathway | 11 | 1 | 1 |
| purine nucleobases degradation II (anaerobic) | 24 | 16 | 2 |
| 10-trans-heptadecenoyl-CoA degradation (reductase-dependent, yeast) | 12 | 2 | 1 |
| 10-cis-heptadecenoyl-CoA degradation (yeast) | 12 | 2 | 1 |
| superpathway of cholesterol degradation III (oxidase) | 49 | 5 | 4 |
| photosynthetic 3-hydroxybutanoate biosynthesis (engineered) | 26 | 20 | 2 |
| folate transformations I | 13 | 9 | 1 |
| formaldehyde assimilation I (serine pathway) | 13 | 6 | 1 |
| (S)-lactate fermentation to propanoate, acetate and hydrogen | 13 | 5 | 1 |
| platensimycin biosynthesis | 26 | 6 | 2 |
| (4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) | 13 | 2 | 1 |
| 1-butanol autotrophic biosynthesis (engineered) | 27 | 20 | 2 |
| docosahexaenoate biosynthesis III (6-desaturase, mammals) | 14 | 2 | 1 |
| mixed acid fermentation | 16 | 12 | 1 |
| heterolactic fermentation | 18 | 16 | 1 |
| superpathway of L-threonine metabolism | 18 | 13 | 1 |
| sitosterol degradation to androstenedione | 18 | 1 | 1 |
| hexitol fermentation to lactate, formate, ethanol and acetate | 19 | 15 | 1 |
| superpathway of methanogenesis | 21 | 2 | 1 |
| superpathway of L-lysine degradation | 43 | 19 | 2 |
| superpathway of N-acetylneuraminate degradation | 22 | 15 | 1 |
| superpathway of ergosterol biosynthesis I | 26 | 4 | 1 |
| superpathway of cholesterol biosynthesis | 38 | 4 | 1 |
| Methanobacterium thermoautotrophicum biosynthetic metabolism | 56 | 21 | 1 |
| arachidonate metabolites biosynthesis | 74 | 3 | 1 |