Experiment set5IT072 for Pseudomonas fluorescens SBW25-INTG

Growth at 10C; with MOPS

Group: temperature
Media: soilextract_PNNL_Prosser_PlotA_B_20191220 + D-Glucose (10 mM) + Ammonium chloride (10 mM) + 3-(N-morpholino)propanesulfonic acid (40 mM), pH=7
Culturing: PseudoSBW25_INTG_ML3, 24-well transparent microplate; Multitron, Aerobic, at 10 (C)
By: Joshua Elmore on 1/6/20

Specific Phenotypes

For 4 genes in this experiment

For temperature D-Glucose in Pseudomonas fluorescens SBW25-INTG

For temperature D-Glucose across organisms

SEED Subsystems

Subsystem	#Specific
2-Ketogluconate Utilization	1
Cyanophycin Metabolism	1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis	1
Glycerol and Glycerol-3-phosphate Uptake and Utilization	1
Queuosine-Archaeosine Biosynthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pentose phosphate pathway
• Alanine and aspartate metabolism
• Glutathione metabolism
• Glycerophospholipid metabolism
• Nitrogen metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-asparagine biosynthesis I	1	1	1
L-glutamine degradation I	1	1	1
L-glutamate biosynthesis I	2	2	1
glycerophosphodiester degradation	2	2	1
phosphatidylcholine resynthesis via glycerophosphocholine	2	1	1
superpathway of L-asparagine biosynthesis	2	1	1
ammonia assimilation cycle III	3	3	1
glycerol and glycerophosphodiester degradation	4	4	1
L-asparagine biosynthesis III (tRNA-dependent)	4	4	1
glutaminyl-tRNAgln biosynthesis via transamidation	4	3	1
phospholipid remodeling (phosphatidylethanolamine, yeast)	4	2	1
glucose degradation (oxidative)	5	5	1
L-glutamate and L-glutamine biosynthesis	7	6	1
L-citrulline biosynthesis	8	8	1
superpathway of L-citrulline metabolism	12	10	1
anandamide biosynthesis I	12	3	1