Experiment set5IT070 for Pseudomonas fluorescens SBW25-INTG

Growth at 10C; with MOPS

Group: temperature
Media: soilextract_PNNL_Prosser_PlotA_B_20191220 + D-Glucose (10 mM) + Ammonium chloride (10 mM) + 3-(N-morpholino)propanesulfonic acid (40 mM), pH=7
Culturing: PseudoSBW25_INTG_ML3, 24-well transparent microplate; Multitron, Aerobic, at 10 (C)
By: Joshua Elmore on 1/6/20

Specific Phenotypes

For 6 genes in this experiment

For temperature D-Glucose in Pseudomonas fluorescens SBW25-INTG

For temperature D-Glucose across organisms

SEED Subsystems

Subsystem	#Specific
Phosphate metabolism	2
2-Ketogluconate Utilization	1
Campylobacter Iron Metabolism	1
Glycerol and Glycerol-3-phosphate Uptake and Utilization	1
High affinity phosphate transporter and control of PHO regulon	1
Queuosine-Archaeosine Biosynthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pentose phosphate pathway
• Glutathione metabolism
• Glycerophospholipid metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
glycerophosphodiester degradation	2	2	1
phosphatidylcholine resynthesis via glycerophosphocholine	2	1	1
glycerol and glycerophosphodiester degradation	4	4	1
phospholipid remodeling (phosphatidylethanolamine, yeast)	4	2	1
glucose degradation (oxidative)	5	5	1
anandamide biosynthesis I	12	3	1