Experiment set5IT068 for Methanococcus maripaludis S2

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Formate with nitrate 62.5 mM

Group: stress
Media: McCas_formate_SO4Free_BicarbFree + Sodium nitrate (62.5 mM), pH=7
Culturing: Methanococcus_S2_ML1, 96 deep-well microplate; 1 mL volume, Anaerobic, at 37 (C)
By: Hans Carlson and Leslie Day on 26-Oct-22
Media components: 10.5 g/L Sodium Chloride, 1 g/L Ammonium chloride, 0.14 g/L Potassium phosphate dibasic, 0.0095 g/L Iron (II) chloride tetrahydrate, 0.001 g/L resazurin sodium salt, 0.84 g/L Sodium acetate, 13.6 g/L Sodium Formate, 2 g/L casamino acids, 0.5 g/L Dithiothreitol, 0.001 g/L Sodium sulfide nonahydrate, MOPS_2M_pH7 (0.002 M 3-(N-morpholino)propanesulfonic acid, 0.0005 M Sodium Hydroxide), Mc_general_salts_SO4free (0.335 g/L Potassium Chloride, 5.6 g/L Magnesium chloride hexahydrate, 0.105 g/L Calcium chloride), Mc_trace_minerals_SO4free (0.021 g/L Trisodium citrate dihydrate, 0.005 g/L Manganese (II) chloride tetrahydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc chloride, 0.0001 g/L copper (II) chloride dihydrate, 0.0001 g/L Aluminum chloride hydrate, 0.0001 g/L Boric Acid, 0.001 g/L Sodium Molybdate Dihydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.002 g/L Sodium selenate, 0.0001 g/L vanadium chloride, 3.3e-05 g/L Sodium tungstate dihydrate), Mc_vitamins (2e-05 g/L biotin, 2e-05 g/L Folic Acid, 0.0001 g/L Pyridoxine HCl, 5e-05 g/L Thiamine HCl, 5e-05 g/L Riboflavin, 5e-05 g/L Nicotinic Acid, 5e-05 g/L calcium pantothenate, 1e-06 g/L Cyanocobalamin, 5e-05 g/L 4-Aminobenzoic acid, 5e-05 g/L Lipoic acid)

Specific Phenotypes

For 10 genes in this experiment

For stress Sodium nitrate in Methanococcus maripaludis S2

For stress Sodium nitrate across organisms

SEED Subsystems

Subsystem #Specific
Alkanesulfonate assimilation 2
Bacterial RNA-metabolizing Zn-dependent hydrolases 1
Conserved gene cluster associated with Met-tRNA formyltransferase 1
Ribosome biogenesis bacterial 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes: