Experiment set5IT067 for Pseudomonas fluorescens FW300-N2C3

LB with Ceftazidime hydrate 0.2 mg/ml

Group: stress
Media: LB + Ceftazidime hydrate (0.2 mg/ml)
Culturing: pseudo5_N2-C3_1_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Adam on 10/8/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 990 F1,F2

Specific Phenotypes

For 5 genes in this experiment

For stress Ceftazidime hydrate in Pseudomonas fluorescens FW300-N2C3

For stress Ceftazidime hydrate across organisms

SEED Subsystems

Subsystem	#Specific
Beta-lactamase	1
Cobalt-zinc-cadmium resistance	1
Peptidoglycan Biosynthesis	1
Tn552	1
Type IV pilus	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Peptidoglycan biosynthesis
• Ascorbate and aldarate metabolism
• Ubiquinone and menaquinone biosynthesis
• Lysine degradation
• Penicillin and cephalosporin biosynthesis
• beta-Lactam resistance
• Nucleotide sugars metabolism
• Glycosaminoglycan degradation
• Biotin metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
peptidoglycan biosynthesis II (staphylococci)	17	12	2
peptidoglycan biosynthesis IV (Enterococcus faecium)	17	12	2
peptidoglycan biosynthesis V (β-lactam resistance)	17	11	2
peptidoglycan biosynthesis I (meso-diaminopimelate containing)	12	12	1
peptidoglycan maturation (meso-diaminopimelate containing)	12	4	1
peptidoglycan biosynthesis III (mycobacteria)	15	11	1