Experiment set5IT065 for Pseudomonas fluorescens FW300-N2C3

LB with Lomefloxacin hydrochloride 0.0005 mM

Group: stress
Media: LB + Lomefloxacin hydrochloride (5e-04 mM)
Culturing: pseudo5_N2-C3_1_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Adam on 10/8/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 990 D1,D2

Specific Phenotypes

For 32 genes in this experiment

For stress Lomefloxacin hydrochloride in Pseudomonas fluorescens FW300-N2C3

For stress Lomefloxacin hydrochloride across organisms

SEED Subsystems

Subsystem	#Specific
DNA repair, bacterial	3
Oxidative stress	3
ATP-dependent RNA helicases, bacterial	1
Acid resistance mechanisms	1
Arginine and Ornithine Degradation	1
Campylobacter Iron Metabolism	1
DNA-replication	1
DNA repair, bacterial DinG and relatives	1
Glutathione: Non-redox reactions	1
Glutathione: Redox cycle	1
Heat shock dnaK gene cluster extended	1
Heme and Siroheme Biosynthesis	1
Lipid A modifications	1
Methylglyoxal Metabolism	1
Orphan regulatory proteins	1
Polyamine Metabolism	1
Queuosine-Archaeosine Biosynthesis	1
Ribosome biogenesis bacterial	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Urea cycle and metabolism of amino groups
• Arginine and proline metabolism
• Aminoacyl-tRNA biosynthesis
• Glutamate metabolism
• Alanine and aspartate metabolism
• Glycine, serine and threonine metabolism
• Phenylalanine metabolism
• Tryptophan metabolism
• Benzoxazinone biosynthesis
• Cyanoamino acid metabolism
• Glutathione metabolism
• Pyruvate metabolism
• Benzoate degradation via CoA ligation
• Styrene degradation
• Porphyrin and chlorophyll metabolism
• Diterpenoid biosynthesis
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
arginine dependent acid resistance	1	1	1
L-glutamine degradation I	1	1	1
glutaminyl-tRNAgln biosynthesis via transamidation	4	4	3
L-asparagine biosynthesis III (tRNA-dependent)	4	4	3
putrescine biosynthesis I	2	2	1
acrylonitrile degradation I	2	2	1
L-glutamate biosynthesis I	2	2	1
indole-3-acetate biosynthesis IV (bacteria)	2	2	1
L-arginine degradation III (arginine decarboxylase/agmatinase pathway)	2	2	1
indole-3-acetate biosynthesis III (bacteria)	2	2	1
L-arginine degradation IV (arginine decarboxylase/agmatine deiminase pathway)	3	3	1
ammonia assimilation cycle III	3	3	1
putrescine biosynthesis II	3	3	1
glutathione-peroxide redox reactions	3	2	1
methylglyoxal degradation VIII	3	2	1
L-arginine degradation X (arginine monooxygenase pathway)	3	2	1
superpathway of acrylonitrile degradation	3	2	1
methylglyoxal degradation I	3	2	1
superpathway of putrescine biosynthesis	4	3	1
spermidine biosynthesis III	4	1	1
L-glutamate and L-glutamine biosynthesis	7	6	1
L-citrulline biosynthesis	8	6	1
superpathway of polyamine biosynthesis I	8	5	1
superpathway of polyamine biosynthesis II	8	5	1
superpathway of methylglyoxal degradation	8	4	1
superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation	11	10	1
superpathway of L-citrulline metabolism	12	8	1
indole-3-acetate biosynthesis II	12	5	1
superpathway of L-arginine and L-ornithine degradation	13	12	1
superpathway of arginine and polyamine biosynthesis	17	14	1