Experiment set5IT064 for Pseudomonas fluorescens FW300-N2E2

Compare to:

LB with rubidium chloride 0.25 M

200 most detrimental genes:

  gene name fitness t score description  
Pf6N2E2_525 +1.4 8.3 C4-type zinc finger protein, DksA/TraR family compare
Pf6N2E2_204 +1.3 2.7 hypothetical protein compare
Pf6N2E2_4057 +1.3 1.6 lipoprotein, putative compare
Pf6N2E2_2580 +1.2 4.5 Flagellar biosynthesis protein FliP compare
Pf6N2E2_3744 +1.2 2.2 General secretion pathway protein F compare
Pf6N2E2_4160 +1.2 2.1 Cytochrome c5 compare
Pf6N2E2_469 +1.1 2.4 lipoprotein, putative compare
Pf6N2E2_1434 +1.1 2.8 Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3) compare
Pf6N2E2_3206 +1.0 0.7 hypothetical protein compare
Pf6N2E2_3362 +1.0 4.1 hypothetical protein compare
Pf6N2E2_962 +1.0 2.4 Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains) compare
Pf6N2E2_2605 +1.0 5.1 3-oxoacyl-[acyl-carrier-protein] synthase, KASIII (EC 2.3.1.180) compare
Pf6N2E2_1113 +1.0 2.8 Transcriptional regulator, TetR family compare
Pf6N2E2_2827 +1.0 1.4 hypothetical protein compare
Pf6N2E2_1987 +0.9 2.5 hypothetical protein compare
Pf6N2E2_750 +0.9 2.3 Transcriptional regulator, DeoR family compare
Pf6N2E2_4615 +0.9 2.5 type IV pili signal transduction protein PilI compare
Pf6N2E2_238 +0.9 1.7 hypothetical protein compare
Pf6N2E2_3659 +0.9 3.7 Protein fixF compare
Pf6N2E2_4994 +0.9 5.3 Nicotinate-nucleotide adenylyltransferase (EC 2.7.7.18) compare
Pf6N2E2_4145 +0.9 3.2 ABC-type transport system involved in resistance to organic solvents, periplasmic component USSDB6C compare
Pf6N2E2_2176 +0.9 2.0 Amidases related to nicotinamidase compare
Pf6N2E2_2878 +0.9 2.7 required for 4-hydroxybenzoate and octanoate transport, together with MFP and FUSC proteins (Pf6N2E2_2877, Pf6N2E2_2879) (from data) compare
Pf6N2E2_3134 +0.9 1.2 hypothetical protein compare
Pf6N2E2_1056 +0.9 0.8 Quinone oxidoreductase (EC 1.6.5.5) compare
Pf6N2E2_961 +0.9 1.6 FIG00954712: hypothetical protein compare
Pf6N2E2_2790 +0.8 2.0 Helix-turn-helix motif compare
Pf6N2E2_1083 +0.8 2.0 hypothetical protein compare
Pf6N2E2_1014 +0.8 1.4 Nitrate/nitrite transporter compare
Pf6N2E2_2447 +0.8 4.5 Probable protease htpX homolog (EC 3.4.24.-) compare
Pf6N2E2_1859 +0.8 2.6 hypothetical protein compare
Pf6N2E2_4365 +0.8 4.1 Ribonuclease PH (EC 2.7.7.56) compare
Pf6N2E2_3021 +0.8 1.3 hypothetical protein compare
Pf6N2E2_2286 +0.8 1.5 FIG00953287: hypothetical protein compare
Pf6N2E2_4364 +0.8 2.6 Protein YicC compare
Pf6N2E2_1336 +0.8 1.8 AraC family transcription regulator compare
Pf6N2E2_5425 +0.8 1.3 hypothetical protein compare
Pf6N2E2_1673 +0.7 1.2 Transcriptional regulator, TetR family compare
Pf6N2E2_971 +0.7 1.5 hypothetical protein compare
Pf6N2E2_687 +0.7 1.3 4-oxalocrotonate tautomerase (EC 5.3.2.-) compare
Pf6N2E2_4459 +0.7 0.7 Transcriptional regulator, GntR family compare
Pf6N2E2_49 +0.7 1.9 FIG00967200: hypothetical protein compare
Pf6N2E2_1549 +0.7 3.0 Transcriptional regulator, LysR family compare
Pf6N2E2_2253 +0.7 2.4 hypothetical protein compare
Pf6N2E2_6046 +0.7 2.6 hypothetical protein compare
Pf6N2E2_2699 +0.7 4.1 Fe(2+)/alpha-ketoglutarate-dependent dioxygenase LpxO compare
Pf6N2E2_3524 +0.7 3.3 hypothetical protein compare
Pf6N2E2_3969 +0.7 1.2 Membrane-fusion protein compare
Pf6N2E2_3635 +0.7 2.1 FIG00953138: hypothetical protein compare
Pf6N2E2_924 +0.7 1.3 Transcriptional regulator XdhR, TetR family compare
Pf6N2E2_2003 +0.7 1.2 hypothetical protein compare
Pf6N2E2_3567 +0.7 1.5 FIG00963947: hypothetical protein compare
Pf6N2E2_2281 +0.7 1.5 Beta N-acetyl-glucosaminidase (EC 3.2.1.52) compare
Pf6N2E2_2864 +0.7 2.1 Gluconokinase (EC 2.7.1.12) compare
Pf6N2E2_2267 +0.7 2.3 Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-) compare
Pf6N2E2_127 +0.7 0.9 AttE component of AttEFGH ABC transport system compare
Pf6N2E2_603 +0.7 1.3 FIG00953934: hypothetical protein compare
Pf6N2E2_1476 +0.7 2.4 (AF179595) Vco33 compare
Pf6N2E2_6029 +0.7 2.0 hypothetical protein compare
Pf6N2E2_3151 +0.7 2.8 Glutamate synthase [NADPH] small chain (EC 1.4.1.13) compare
Pf6N2E2_5847 +0.7 3.0 Glycine betaine/L-proline ABC transporter, glycine betaine/L-proline- binding/permease protein compare
Pf6N2E2_2013 +0.7 1.2 hypothetical protein compare
Pf6N2E2_5086 +0.7 1.1 hypothetical protein compare
Pf6N2E2_1462 +0.6 2.7 Nicotinamidase/isochorismatase family protein compare
Pf6N2E2_3568 +0.6 2.1 Unsaturated fatty acid biosythesis repressor FabR, TetR family compare
Pf6N2E2_477 +0.6 2.4 Branched-chain amino acid aminotransferase (EC 2.6.1.42) compare
Pf6N2E2_1319 +0.6 2.2 FIG074102: hypothetical protein compare
Pf6N2E2_1329 +0.6 1.8 Phenylacetaldehyde dehydrogenase (EC 1.2.1.39) compare
Pf6N2E2_3664 +0.6 3.9 Carbamoyltransferase in large core OS assembly cluster compare
Pf6N2E2_1009 +0.6 1.6 Inositol transport system permease protein compare
Pf6N2E2_1294 +0.6 1.6 Carbon starvation protein A compare
Pf6N2E2_3725 +0.6 1.6 FIG085779: Lipoprotein compare
Pf6N2E2_2591 +0.6 2.2 Flagellar assembly protein FliH compare
Pf6N2E2_5768 +0.6 2.4 DNA recombination-dependent growth factor C compare
Pf6N2E2_1715 +0.6 1.7 hypothetical protein compare
Pf6N2E2_1688 +0.6 2.4 Permeases of the major facilitator superfamily compare
Pf6N2E2_5262 +0.6 1.2 FKBP-type peptidyl-prolyl cis-trans isomerase SlyD (EC 5.2.1.8) compare
Pf6N2E2_37 +0.6 1.9 6-carboxytetrahydropterin synthase (EC 4.1.2.50) @ Queuosine biosynthesis QueD, PTPS-I compare
Pf6N2E2_2428 +0.6 1.5 Thiol-disulfide isomerase and thioredoxins compare
Pf6N2E2_3373 +0.6 2.5 Outer membrane lipoprotein SmpA, a component of the essential YaeT outer-membrane protein assembly complex compare
Pf6N2E2_136 +0.6 1.6 Glycolate dehydrogenase (EC 1.1.99.14), FAD-binding subunit GlcE compare
Pf6N2E2_288 +0.6 1.4 Isocitrate dehydrogenase [NADP] (EC 1.1.1.42); Monomeric isocitrate dehydrogenase [NADP] (EC 1.1.1.42) compare
Pf6N2E2_4307 +0.6 1.1 Type VI secretion lipoprotein/VasD compare
Pf6N2E2_1316 +0.6 2.0 P-hydroxyphenylacetate hydroxylase C1:reductase component compare
Pf6N2E2_529 +0.6 2.3 FIG00954439: hypothetical protein compare
Pf6N2E2_2296 +0.6 1.9 FIG00954767: hypothetical protein compare
Pf6N2E2_2871 +0.6 2.1 FIG00954271: hypothetical protein compare
Pf6N2E2_3060 +0.6 1.4 Outer membrane protein H precursor compare
Pf6N2E2_1322 +0.6 1.7 MoaE protein compare
Pf6N2E2_2798 +0.6 2.6 Death on curing protein, Doc toxin compare
Pf6N2E2_724 +0.6 1.5 Nitric oxide -responding transcriptional regulator Dnr (Crp/Fnr family) compare
Pf6N2E2_3226 +0.6 1.6 Predicted D-glucarate or D-galactorate regulator, GntR family compare
Pf6N2E2_967 +0.6 2.4 DNA repair protein RadC compare
Pf6N2E2_1490 +0.6 1.3 hypothetical protein compare
Pf6N2E2_159 +0.6 2.1 DNA damage resistance protein (from data) compare
Pf6N2E2_3947 +0.6 1.4 Cd(II)/Pb(II)-responsive transcriptional regulator compare
Pf6N2E2_530 +0.6 3.1 Histidine transporter, periplasmic histidine-binding protein compare
Pf6N2E2_2498 +0.6 3.2 Transcriptional regulator, GntR family compare
Pf6N2E2_3072 +0.6 3.9 [Protein-PII] uridylyltransferase (EC 2.7.7.59) compare
Pf6N2E2_2606 +0.6 3.0 hypothetical protein compare
Pf6N2E2_5757 +0.6 1.8 hypothetical protein compare
Pf6N2E2_4422 +0.6 1.9 Frataxin homolog CyaY, facilitates iron supply for heme A synthesis or Fe-S cluster assembly compare
Pf6N2E2_2213 +0.5 2.7 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf6N2E2_5993 +0.5 0.7 FIG00957412: hypothetical protein compare
Pf6N2E2_4275 +0.5 2.0 hypothetical protein compare
Pf6N2E2_4843 +0.5 2.2 FIG00956941: hypothetical protein compare
Pf6N2E2_2535 +0.5 3.1 FIG00954136: hypothetical protein compare
Pf6N2E2_3009 +0.5 1.1 Phage tail tube protein compare
Pf6N2E2_3763 +0.5 1.5 hypothetical protein compare
Pf6N2E2_4607 +0.5 2.1 Uracil phosphoribosyltransferase (EC 2.4.2.9) / Pyrimidine operon regulatory protein PyrR compare
Pf6N2E2_1283 +0.5 1.5 Ferric siderophore transport system, periplasmic binding protein TonB compare
Pf6N2E2_1933 +0.5 1.6 TcuR: regulates tcuABC genes used in utilization of tricarballylate compare
Pf6N2E2_4300 +0.5 1.0 Uncharacterized protein similar to VCA0109 compare
Pf6N2E2_665 +0.5 1.4 Acetoin dehydrogenase E1 component alpha-subunit (EC 1.2.4.-) compare
Pf6N2E2_1536 +0.5 2.1 Permeases of the major facilitator superfamily compare
Pf6N2E2_3613 +0.5 3.4 GTP-binding protein HflX compare
Pf6N2E2_2123 +0.5 1.9 hypothetical protein compare
Pf6N2E2_2065 +0.5 1.7 Cobalt-precorrin-4 C11-methyltransferase (EC 2.1.1.133) compare
Pf6N2E2_4332 +0.5 2.2 hypothetical protein compare
Pf6N2E2_3283 +0.5 2.1 Cytoplasmic axial filament protein CafA and Ribonuclease G (EC 3.1.4.-) compare
Pf6N2E2_638 +0.5 1.0 4-carboxymuconolactone decarboxylase domain/alkylhydroperoxidase AhpD family core domain protein compare
Pf6N2E2_160 +0.5 1.4 hypothetical protein compare
Pf6N2E2_5520 +0.5 2.1 Sensory box histidine kinase compare
Pf6N2E2_403 +0.5 2.3 Cyanate hydratase (EC 4.2.1.104) compare
Pf6N2E2_4987 +0.5 0.7 MaoC-like domain protein compare
Pf6N2E2_6089 +0.5 2.1 ThiJ/PfpI family protein compare
Pf6N2E2_5876 +0.5 1.4 Lipase (EC 3.1.1.3) compare
Pf6N2E2_5643 +0.5 1.9 hypothetical protein compare
Pf6N2E2_1085 +0.5 1.7 Fe2+/Zn2+ uptake regulation proteins compare
Pf6N2E2_6049 +0.5 1.8 hydrolase, haloacid dehalogenase-like family compare
Pf6N2E2_1170 +0.5 2.1 Methyl-accepting chemotaxis protein I (serine chemoreceptor protein) compare
Pf6N2E2_1293 +0.5 0.6 COG2879, Hypothetical small protein yjiX compare
Pf6N2E2_2578 +0.5 1.0 Flagellar biosynthesis protein FliR compare
Pf6N2E2_1309 +0.5 2.5 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase (EC 1.2.1.60) compare
Pf6N2E2_5772 +0.5 2.0 DUF1232 domain-containing protein compare
Pf6N2E2_4418 +0.5 0.5 Diaminopimelate epimerase (EC 5.1.1.7) compare
Pf6N2E2_3240 +0.5 0.9 Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63) compare
Pf6N2E2_4635 +0.5 2.9 ATP-dependent RNA helicase RhlE compare
Pf6N2E2_1983 +0.5 1.6 hypothetical protein compare
Pf6N2E2_3344 +0.5 2.0 N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28) AmpD compare
Pf6N2E2_1618 +0.5 2.5 ABC-type amino acid transport, signal transduction systems, periplasmic component/domain compare
Pf6N2E2_4143 +0.5 2.1 ABC-type transport system involved in resistance to organic solvents, permease component USSDB6A compare
Pf6N2E2_3277 +0.5 1.5 FagA protein compare
Pf6N2E2_4298 +0.5 1.6 Uncharacterized protein ImpB compare
Pf6N2E2_695 +0.5 1.7 Nitrite reductase associated c-type cytochorome NirN compare
Pf6N2E2_1455 +0.5 2.0 Xylose ABC transporter, periplasmic xylose-binding protein XylF compare
Pf6N2E2_2012 +0.5 1.3 hypothetical protein compare
Pf6N2E2_5694 +0.5 2.0 Flagellar basal-body rod protein FlgB compare
Pf6N2E2_2778 +0.5 1.0 Urea carboxylase-related aminomethyltransferase (EC 2.1.2.10) compare
Pf6N2E2_5505 +0.5 1.4 hypothetical protein compare
Pf6N2E2_2934 +0.5 1.4 lipoprotein, putative compare
Pf6N2E2_2734 +0.5 1.7 Transcriptional regulator, LysR family compare
Pf6N2E2_2369 +0.5 1.3 Flagellum-specific ATP synthase FliI compare
Pf6N2E2_1303 +0.5 1.2 TRAP-type transport system, small permease component, predicted N-acetylneuraminate transporter compare
Pf6N2E2_2396 +0.5 1.8 Sensor histidine kinase compare
Pf6N2E2_5573 +0.5 1.8 Glycerol-3-phosphate regulon repressor, DeoR family compare
Pf6N2E2_1005 +0.5 2.2 Ribose ABC transport system, periplasmic ribose-binding protein RbsB (TC 3.A.1.2.1) compare
Pf6N2E2_2579 +0.5 2.5 Flagellar biosynthesis protein FliQ compare
Pf6N2E2_2597 +0.5 3.1 Flagellar regulatory protein FleQ compare
Pf6N2E2_5182 +0.5 2.3 Paraquat-inducible protein A compare
Pf6N2E2_1477 +0.5 2.0 hypothetical protein compare
Pf6N2E2_81 +0.5 1.0 hypothetical protein compare
Pf6N2E2_6055 +0.5 1.7 Homoserine kinase (EC 2.7.1.39) compare
Pf6N2E2_1286 +0.5 2.3 Hemolysin activation/secretion protein associated with VreARI signalling system compare
Pf6N2E2_5225 +0.5 3.4 Lipid A 3-O-deacylase compare
Pf6N2E2_2518 +0.5 0.9 Cyclohexadienyl dehydrogenase (EC 1.3.1.12)(EC 1.3.1.43) / 5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19) compare
Pf6N2E2_5711 +0.5 2.3 tRNA(Cytosine32)-2-thiocytidine synthetase compare
Pf6N2E2_763 +0.5 2.0 Sigma-54 dependent transcriptional regulator compare
Pf6N2E2_1248 +0.5 1.1 MutT/nudix family protein compare
Pf6N2E2_2254 +0.5 1.3 Cell filamentation protein fic compare
Pf6N2E2_1762 +0.5 1.7 putative lipase compare
Pf6N2E2_4009 +0.5 3.0 GGDEF domain protein compare
Pf6N2E2_3454 +0.5 1.1 hypothetical protein compare
Pf6N2E2_2577 +0.5 2.5 Flagellar biosynthesis protein FlhB compare
Pf6N2E2_5223 +0.5 2.1 Molybdopterin biosynthesis protein MoeB compare
Pf6N2E2_27 +0.4 1.7 Mechanosensitive ion channel compare
Pf6N2E2_4464 +0.4 1.1 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases / CDP-6-deoxy-delta-3,4-glucoseen reductase-like compare
Pf6N2E2_1209 +0.4 2.2 hypothetical protein compare
Pf6N2E2_5017 +0.4 2.3 Phosphate starvation-inducible protein PhoH, predicted ATPase compare
Pf6N2E2_5538 +0.4 2.4 4-hydroxybenzoyl-CoA thioesterase family active site compare
Pf6N2E2_2910 +0.4 1.9 Carbamate kinase (EC 2.7.2.2) compare
Pf6N2E2_1579 +0.4 1.7 hypothetical protein compare
Pf6N2E2_865 +0.4 0.8 Fumarylacetoacetate hydrolase family protein compare
Pf6N2E2_1617 +0.4 0.6 COG2199: FOG: GGDEF domain compare
Pf6N2E2_1446 +0.4 1.7 Enoyl-[acyl-carrier-protein] reductase [NADPH] (EC 1.3.1.10) compare
Pf6N2E2_2010 +0.4 0.8 hypothetical protein compare
Pf6N2E2_786 +0.4 1.2 Type-1 fimbrial protein, A chain precursor compare
Pf6N2E2_1399 +0.4 1.9 Transcriptional regulator, LuxR family compare
Pf6N2E2_5903 +0.4 1.9 5-amino-6-(5-phosphoribosylamino)uracil reductase (EC 1.1.1.193) compare
Pf6N2E2_3338 +0.4 1.0 1-phosphofructokinase (EC 2.7.1.56) compare
Pf6N2E2_6034 +0.4 1.9 FIG00965471: hypothetical protein compare
Pf6N2E2_567 +0.4 2.2 Universal stress protein family 7 compare
Pf6N2E2_946 +0.4 1.1 Transcriptional regulator, LysR family compare
Pf6N2E2_2367 +0.4 0.8 hypothetical protein compare
Pf6N2E2_1188 +0.4 1.4 Alpha-ketoglutarate-dependent taurine dioxygenase (EC 1.14.11.17) compare
Pf6N2E2_1671 +0.4 1.6 Transcriptional regulator, TetR family compare
Pf6N2E2_796 +0.4 2.3 Fe(2+)/alpha-ketoglutarate-dependent dioxygenase LpxO compare
Pf6N2E2_380 +0.4 2.3 putative ABC transporter, periplasmic substrate-binding protein compare
Pf6N2E2_1011 +0.4 1.7 hypothetical protein compare
Pf6N2E2_2433 +0.4 1.4 Probable transmembrane protein compare


Specific Phenotypes

For 2 genes in this experiment

For stress rubidium chloride in Pseudomonas fluorescens FW300-N2E2

For stress rubidium chloride across organisms