Experiment set5IT063 for Sphingomonas koreensis DSMZ 15582
L-Glutamic acid monopotassium salt monohydrate nitrogen source
Group: nitrogen sourceMedia: RCH2_defined_Glucose_noNitrogen + L-Glutamic acid monopotassium salt monohydrate (5 mM)
Culturing: korea_ML2, tube, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 5.8 generations
By: Mark on 1/26/2015
Media components: 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 20 mM D-Glucose, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Specific Phenotypes
For 1 genes in this experiment
For nitrogen source L-Glutamic acid monopotassium salt monohydrate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Glycerol and Glycerol-3-phosphate Uptake and Utilization | 1 |
| Glycerolipid and Glycerophospholipid Metabolism in Bacteria | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| glycerol-3-phosphate shuttle | 2 | 2 | 1 |
| CDP-diacylglycerol biosynthesis I | 4 | 4 | 1 |
| CDP-diacylglycerol biosynthesis II | 4 | 4 | 1 |
| CDP-diacylglycerol biosynthesis III | 5 | 4 | 1 |
| phosphatidate biosynthesis (yeast) | 5 | 3 | 1 |
| glucosylglycerol biosynthesis | 5 | 2 | 1 |
| phosphatidylglycerol biosynthesis I | 6 | 5 | 1 |
| phosphatidylglycerol biosynthesis II | 6 | 5 | 1 |
| 1,3-propanediol biosynthesis (engineered) | 9 | 4 | 1 |
| superpathway of phospholipid biosynthesis III (E. coli) | 12 | 9 | 1 |
| superpathway of cardiolipin biosynthesis (bacteria) | 13 | 9 | 1 |
| superpathway of phospholipid biosynthesis II (plants) | 28 | 9 | 1 |