Experiment set5IT061 for Pseudomonas fluorescens FW300-N2C3

LB with 1-ethyl-3-methylimidazolium chloride 200 mM

Group: stress
Media: LB + 1-ethyl-3-methylimidazolium chloride (200 mM)
Culturing: pseudo5_N2-C3_1_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Adam on 10/8/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 989 E3,E4

Specific Phenotypes

For 40 genes in this experiment

For stress 1-ethyl-3-methylimidazolium chloride in Pseudomonas fluorescens FW300-N2C3

For stress 1-ethyl-3-methylimidazolium chloride across organisms

SEED Subsystems

Subsystem	#Specific
Siderophore Pyoverdine	10
Choline and Betaine Uptake and Betaine Biosynthesis	3
Iron acquisition in Vibrio	3
Arginine and Ornithine Degradation	2
Oxidative stress	2
Polyamine Metabolism	2
Acid resistance mechanisms	1
Biogenesis of cbb3-type cytochrome c oxidases	1
Carboxysome	1
Respiratory dehydrogenases 1	1
Rhamnose containing glycans	1
Terminal cytochrome C oxidases	1
Transport of Iron	1
dTDP-rhamnose synthesis	1
linker unit-arabinogalactan synthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Arginine and proline metabolism
• Urea cycle and metabolism of amino groups
• Lysine biosynthesis
• Nucleotide sugars metabolism
• Caprolactam degradation
• Oxidative phosphorylation
• Caffeine metabolism
• Glycine, serine and threonine metabolism
• Methionine metabolism
• Lysine degradation
• Tyrosine metabolism
• Aminophosphonate metabolism
• Glutathione metabolism
• Streptomycin biosynthesis
• Polyketide sugar unit biosynthesis
• Lipopolysaccharide biosynthesis
• Sphingolipid metabolism
• 1,4-Dichlorobenzene degradation
• Nicotinate and nicotinamide metabolism
• Porphyrin and chlorophyll metabolism
• Nitrogen metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
putrescine biosynthesis II	3	3	3
L-arginine degradation IV (arginine decarboxylase/agmatine deiminase pathway)	3	3	3
arginine dependent acid resistance	1	1	1
L-arginine degradation III (arginine decarboxylase/agmatinase pathway)	2	2	1
NADH to cytochrome bo oxidase electron transfer I	2	2	1
putrescine biosynthesis I	2	2	1
NADH to cytochrome bo oxidase electron transfer II	2	2	1
NADH to nitrate electron transfer	2	1	1
nitrate reduction VIIIb (dissimilatory)	2	1	1
NADH to cytochrome aa3 oxidase electron transfer	2	1	1
NADH to cytochrome bd oxidase electron transfer II	2	1	1
ferrichrome biosynthesis	2	1	1
NADH to cytochrome bd oxidase electron transfer I	2	1	1
superpathway of polyamine biosynthesis II	8	5	3
aerobic respiration III (alternative oxidase pathway)	3	3	1
pyoverdine I biosynthesis	11	7	3
aerobic respiration II (cytochrome c) (yeast)	4	3	1
aerobic respiration I (cytochrome c)	4	3	1
superpathway of putrescine biosynthesis	4	3	1
spermidine biosynthesis III	4	1	1
ectoine degradation	4	1	1
dTDP-β-L-rhamnose biosynthesis	5	5	1
mitochondrial NADPH production (yeast)	5	4	1
ectoine biosynthesis	5	3	1
ferrichrome A biosynthesis	5	2	1
norspermidine biosynthesis	6	4	1
NAD(P)/NADPH interconversion	6	3	1
Fe(II) oxidation	6	3	1
rhizobactin 1021 biosynthesis	7	1	1
superpathway of NAD/NADP - NADH/NADPH interconversion (yeast)	8	7	1
superpathway of polyamine biosynthesis I	8	5	1
superpathway of polyamine biosynthesis III	8	4	1
superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation	11	10	1
O-antigen building blocks biosynthesis (E. coli)	11	10	1
mycobactin biosynthesis	11	3	1
baumannoferrin biosynthesis	11	1	1
superpathway of L-arginine and L-ornithine degradation	13	12	1
superpathway of arginine and polyamine biosynthesis	17	14	1
superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis	19	5	1
superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis	33	12	1