Experiment set5IT059 for Pseudomonas fluorescens FW300-N2C3
LB with 1-ethyl-3-methylimidazolium acetate 10 mM
Group: stressMedia: LB + 1-ethyl-3-methylimidazolium acetate (10 mM)
Culturing: pseudo5_N2-C3_1_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Adam on 10/8/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 989 D3,D4
Specific Phenotypes
For 4 genes in this experiment
For stress 1-ethyl-3-methylimidazolium acetate in Pseudomonas fluorescens FW300-N2C3
For stress 1-ethyl-3-methylimidazolium acetate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Methionine Salvage | 1 |
| Molybdenum cofactor biosynthesis | 1 |
| Riboflavin, FMN and FAD metabolism | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Riboflavin metabolism
- Methionine metabolism
- Geraniol degradation
- Pyruvate metabolism
- 1- and 2-Methylnaphthalene degradation
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| 3-methylthiopropanoate biosynthesis | 1 | 1 | 1 |
| flavin biosynthesis I (bacteria and plants) | 9 | 8 | 2 |
| flavin biosynthesis III (fungi) | 9 | 7 | 2 |
| 6-hydroxymethyl-dihydropterin diphosphate biosynthesis III (Chlamydia) | 5 | 3 | 1 |
| toxoflavin biosynthesis | 7 | 3 | 1 |
| S-methyl-5-thio-α-D-ribose 1-phosphate degradation I | 7 | 2 | 1 |
| flavin biosynthesis II (archaea) | 10 | 5 | 1 |
| L-methionine salvage cycle III | 11 | 5 | 1 |
| L-methionine salvage cycle II (plants) | 11 | 3 | 1 |
| L-methionine salvage cycle I (bacteria and plants) | 12 | 4 | 1 |