Experiment set5IT057 for Bacteroides thetaiotaomicron VPI-5482

Defined media with Bile salts 0.5 mg/ml

Group: stress
Media: Varel_Bryant_medium_Glucose + Bile salts (0.5 mg/ml)
Culturing: Btheta_ML6a, 24-well transparent microplate, Anaerobic, at 37 (C), shaken=0 rpm
By: Hualan on 12/19/17
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, 20 mM D-Glucose, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)
Growth plate: 1947 D5

Specific Phenotypes

For 12 genes in this experiment

For stress Bile salts in Bacteroides thetaiotaomicron VPI-5482

For stress Bile salts across organisms

SEED Subsystems

Subsystem	#Specific
Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria	3
Peptidyl-prolyl cis-trans isomerase	1
Predicted carbohydrate hydrolases	1
Protein degradation	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.