Experiment set5IT055 for Pseudomonas fluorescens FW300-N2E2

LB with Hydroxylamine hydrochloride 0.5 M

Group: stress
Media: LB + Hydroxylamine hydrochloride (0.5 M), pH=7
Culturing: pseudo6_N2E2_ML5, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=500 rpm
By: Hans_Hualan on 2/2/2016
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 24 genes in this experiment

For stress Hydroxylamine hydrochloride in Pseudomonas fluorescens FW300-N2E2

For stress Hydroxylamine hydrochloride across organisms

SEED Subsystems

Subsystem	#Specific
Arginine and Ornithine Degradation	5
Experimental tye	2
Heme and Siroheme Biosynthesis	2
Terminal cytochrome C oxidases	2
Biogenesis of cytochrome c oxidases	1
Glutamate dehydrogenases	1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis	1
Hemin transport system	1
Lipid A modifications	1
Peptidoglycan Biosynthesis	1
Rrf2 family transcriptional regulators	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Lipopolysaccharide biosynthesis
• Porphyrin and chlorophyll metabolism
• Ascorbate and aldarate metabolism
• Ubiquinone and menaquinone biosynthesis
• Oxidative phosphorylation
• Purine metabolism
• Glutamate metabolism
• Glycine, serine and threonine metabolism
• Arginine and proline metabolism
• Benzoxazinone biosynthesis
• Nucleotide sugars metabolism
• Glycosaminoglycan degradation
• Folate biosynthesis
• Diterpenoid biosynthesis
• Nitrogen metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-glutamate degradation I	1	1	1
tetrapyrrole biosynthesis II (from glycine)	4	3	2
arsenite to oxygen electron transfer	2	1	1
tetrapyrrole biosynthesis I (from glutamate)	6	6	2
L-alanine degradation II (to D-lactate)	3	3	1
ethene biosynthesis IV (engineered)	3	1	1
arsenite to oxygen electron transfer (via azurin)	3	1	1
superpathway of heme b biosynthesis from glycine	8	7	2
aerobic respiration I (cytochrome c)	4	3	1
aerobic respiration II (cytochrome c) (yeast)	4	3	1
superpathway of heme b biosynthesis from glutamate	10	10	2
Fe(II) oxidation	6	3	1
L-glutamate degradation XI (reductive Stickland reaction)	7	3	1
4-aminobutanoate degradation V	7	2	1
L-glutamate degradation V (via hydroxyglutarate)	10	6	1
superpathway of bacteriochlorophyll a biosynthesis	26	6	2
adenosylcobalamin biosynthesis II (aerobic)	33	31	2
adenosylcobalamin biosynthesis I (anaerobic)	36	29	2
methylaspartate cycle	19	9	1