Experiment set5IT055 for Pseudomonas fluorescens FW300-N2C3

LB with Cholin acetate 40 mM

Group: stress
Media: LB + Cholin acetate (40 mM)
Culturing: pseudo5_N2-C3_1_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Adam on 10/8/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 989 B3,B4

Specific Phenotypes

For 13 genes in this experiment

For stress Cholin acetate in Pseudomonas fluorescens FW300-N2C3

For stress Cholin acetate across organisms

SEED Subsystems

Subsystem	#Specific
Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria	2
Choline and Betaine Uptake and Betaine Biosynthesis	1
Methionine Salvage	1
Molybdenum cofactor biosynthesis	1
Riboflavin, FMN and FAD metabolism	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Riboflavin metabolism
• Glycine, serine and threonine metabolism
• Methionine metabolism
• Geraniol degradation
• Pyruvate metabolism
• 1- and 2-Methylnaphthalene degradation

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
3-methylthiopropanoate biosynthesis	1	1	1
choline degradation I	2	2	1
glycine betaine biosynthesis I (Gram-negative bacteria)	2	2	1
glycine betaine biosynthesis II (Gram-positive bacteria)	2	2	1
choline-O-sulfate degradation	3	3	1
flavin biosynthesis I (bacteria and plants)	9	8	2
flavin biosynthesis III (fungi)	9	7	2
6-hydroxymethyl-dihydropterin diphosphate biosynthesis III (Chlamydia)	5	3	1
toxoflavin biosynthesis	7	3	1
S-methyl-5-thio-α-D-ribose 1-phosphate degradation I	7	2	1
flavin biosynthesis II (archaea)	10	5	1
L-methionine salvage cycle III	11	5	1
L-methionine salvage cycle II (plants)	11	3	1
L-methionine salvage cycle I (bacteria and plants)	12	4	1