Experiment set5IT055 for Pseudomonas fluorescens SBW25-INTG

Sodium nitrite nitrogen source

Group: nitrogen source
Media: soilextract_PNNL_Prosser_PlotA_B_20191220 + D-Glucose (10 mM) + Sodium nitrite (10 mM)
Culturing: PseudoSBW25_INTG_ML3, 96 deep-well microplate; 1 mL volume, Aerobic, at 30 (C)
By: Joshua Elmore on 1/6/20

Specific Phenotypes

For 8 genes in this experiment

For nitrogen source D-Glucose in Pseudomonas fluorescens SBW25-INTG

For nitrogen source D-Glucose across organisms

SEED Subsystems

Subsystem	#Specific
Nitrate and nitrite ammonification	2
Carboxysome	1
Cyanate hydrolysis	1
Methionine Biosynthesis	1
Thiamin biosynthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Nitrogen metabolism
• Thiamine metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
glyphosate degradation II	1	1	1
alkylnitronates degradation	2	2	1
CO2 fixation into oxaloacetate (anaplerotic)	2	2	1
nitrate reduction V (assimilatory)	2	1	1
cyanate degradation	3	2	1
thiazole component of thiamine diphosphate biosynthesis II	7	5	1
C4 photosynthetic carbon assimilation cycle, NADP-ME type	7	4	1
butanol and isobutanol biosynthesis (engineered)	8	3	1
superpathway of thiamine diphosphate biosynthesis II	11	9	1
C4 photosynthetic carbon assimilation cycle, NAD-ME type	11	6	1
3-hydroxypropanoate cycle	13	6	1
glyoxylate assimilation	13	4	1
C4 photosynthetic carbon assimilation cycle, PEPCK type	14	9	1
gluconeogenesis II (Methanobacterium thermoautotrophicum)	18	9	1
3-hydroxypropanoate/4-hydroxybutanate cycle	18	8	1
superpathway of the 3-hydroxypropanoate cycle	18	6	1
Methanobacterium thermoautotrophicum biosynthetic metabolism	56	20	1