Experiment set5IT054 for Desulfovibrio vulgaris Hildenborough JW710
Formate/Acetate-Sulfate (60/30-30 mM) with Casamino acids 0.05% without Molybdate/Tungstate
Group: respiratory growthMedia: Dv_base_medium_no_Mo_no_W + Sodium Formate (60 mM) + Sodium acetate (30 mM) + Sodium sulfate (30 mM) + casamino acids (0.5 mg/mL), pH=7.2
Culturing: DvH_JW710, 15 mL tube, Anaerobic, at 30 (C), shaken=0 rpm
By: Valentine on 11/15/2016
Media components: 20 mM Ammonium chloride, 30 mM Tris hydrochloride, 0.12 mM EDTA, 1 mM Sodium sulfide nonahydrate, 8 mM Magnesium chloride hexahydrate, 0.6 mM Calcium chloride, 2 mM Potassium phosphate dibasic, 60 uM Iron (II) chloride tetrahydrate, Desulfovibrio trace elements minus Sodium molybdate minus Sodium tungstate dihydrate (15 uM Manganese (II) chloride tetrahydrate, 7.8 uM Cobalt chloride hexahydrate, 9 uM Zinc chloride, 1.92 uM Boric Acid, 2.28 uM Nickel (II) sulfate hexahydrate, 0.06 uM copper (II) chloride dihydrate, 0.21 uM Sodium selenite pentahydrate), Thauer's vitamin mix (0.01 mg/L Pyridoxine HCl, 0.005 mg/L 4-Aminobenzoic acid, 0.005 mg/L Lipoic acid, 0.005 mg/L Nicotinic Acid, 0.005 mg/L Riboflavin, 0.005 mg/L Thiamine HCl, 0.005 mg/L calcium pantothenate, 0.002 mg/L biotin, 0.002 mg/L Folic Acid, 0.0001 mg/L Cyanocobalamin, 0.2 mg/L Choline chloride)
Specific Phenotypes
None in this experiment
For Desulfovibrio vulgaris Hildenborough JW710 in respiratory growth experiments