Experiment set5IT049 for Pseudomonas fluorescens SBW25-INTG

Growth at 34C; with MOPS

Group: temperature
Media: soilextract_PNNL_Prosser_PlotA_B_20191220 + D-Glucose (10 mM) + Ammonium chloride (10 mM) + 3-(N-morpholino)propanesulfonic acid (40 mM), pH=7
Culturing: PseudoSBW25_INTG_ML3, 24-well transparent microplate; Multitron, Aerobic, at 34 (C)
By: Joshua Elmore on 1/6/20

Specific Phenotypes

For 5 genes in this experiment

For temperature D-Glucose in Pseudomonas fluorescens SBW25-INTG

For temperature D-Glucose across organisms

SEED Subsystems

Subsystem	#Specific
High affinity phosphate transporter and control of PHO regulon	2
Phosphate metabolism	2
ABC transporter alkylphosphonate (TC 3.A.1.9.1)	1
Peptidoglycan Biosynthesis	1
Type IV pilus	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Peptidoglycan biosynthesis
• Lysine degradation
• Biotin metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
peptidoglycan maturation (meso-diaminopimelate containing)	12	4	3
peptidoglycan biosynthesis IV (Enterococcus faecium)	17	12	2
peptidoglycan biosynthesis II (staphylococci)	17	12	2
peptidoglycan biosynthesis V (β-lactam resistance)	17	11	2
peptidoglycan biosynthesis I (meso-diaminopimelate containing)	12	12	1
peptidoglycan biosynthesis III (mycobacteria)	15	11	1