Experiment set5IT049 for Sphingomonas koreensis DSMZ 15582

LB with Fusidic acid sodium salt 0.0005 mg/ml

Group: stress
Media: LB + Fusidic acid sodium salt (5e-04 mg/ml)
Culturing: korea_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 28 (C), shaken=orbital
By: Mark on 1/26/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 1114 E7,E8

Specific Phenotypes

For 13 genes in this experiment

For stress Fusidic acid sodium salt in Sphingomonas koreensis DSMZ 15582

For stress Fusidic acid sodium salt across organisms

SEED Subsystems

Subsystem	#Specific
Peptidoglycan Biosynthesis	2
Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria	1
Multidrug Resistance Efflux Pumps	1
Ton and Tol transport systems	1
Type IV pilus	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Peptidoglycan biosynthesis
• Lipopolysaccharide biosynthesis
• Fructose and mannose metabolism
• Ascorbate and aldarate metabolism
• Ubiquinone and menaquinone biosynthesis
• Lysine degradation
• N-Glycan biosynthesis
• O-Glycan biosynthesis
• High-mannose type N-glycan biosynthesis
• O-Mannosyl glycan biosynthesis
• Nucleotide sugars metabolism
• Glycosaminoglycan degradation
• Keratan sulfate biosynthesis
• Glycerolipid metabolism
• Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
• Sphingolipid metabolism
• Glycosphingolipid biosynthesis - lacto and neolacto series
• Glycosphingolipid biosynthesis - globo series
• Glycosphingolipid biosynthesis - ganglio series
• Biotin metabolism
• Carotenoid biosynthesis - General
• Zeatin biosynthesis
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Flavone and flavonol biosynthesis

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
peptidoglycan biosynthesis IV (Enterococcus faecium)	17	12	2
peptidoglycan biosynthesis II (staphylococci)	17	12	2
peptidoglycan biosynthesis V (β-lactam resistance)	17	11	2
peptidoglycan biosynthesis I (meso-diaminopimelate containing)	12	11	1
peptidoglycan maturation (meso-diaminopimelate containing)	12	4	1
peptidoglycan biosynthesis III (mycobacteria)	15	11	1