Experiment set5IT048 for Pseudomonas fluorescens FW300-N1B4

Compare to:

L-Serine nitrogen source

200 most detrimental genes:

  gene name fitness t score description  
Pf1N1B4_5224 +3.0 7.5 tRNA-Arg-CCT compare
Pf1N1B4_3501 +2.9 15.8 BarA sensory histidine kinase (= VarS = GacS) compare
Pf1N1B4_5619 +2.3 4.9 BarA-associated response regulator UvrY (= GacA = SirA) compare
Pf1N1B4_623 +2.3 7.1 Aminomethyltransferase (glycine cleavage system T protein) (EC 2.1.2.10) compare
Pf1N1B4_218 +2.1 12.9 Periplasmic binding protein compare
Pf1N1B4_3115 +1.5 10.3 RNA polymerase sigma factor RpoS compare
Pf1N1B4_7 +1.4 8.4 tRNA pseudouridine synthase A (EC 4.2.1.70) compare
Pf1N1B4_1463 +1.2 9.1 hypothetical protein compare
Pf1N1B4_2169 +1.1 5.5 FIG00953808: hypothetical protein compare
Pf1N1B4_904 +1.1 4.3 DNA-binding protein HU-beta compare
Pf1N1B4_1456 +1.1 8.4 Hypothetical protein FIG015671 in large core OS assembly cluster compare
Pf1N1B4_2493 +1.1 3.8 Acyl-CoA dehydrogenase (EC 1.3.8.7) compare
Pf1N1B4_1466 +1.1 5.0 hypothetical protein compare
Pf1N1B4_2439 +1.1 7.5 DgcA Dimethylglycine demethylase subunit A conserved
Pf1N1B4_2437 +1.1 4.5 Microsomal dipeptidase (EC 3.4.13.19) compare
Pf1N1B4_5927 +1.1 9.9 Capsular polysaccharide biosynthesis protein compare
Pf1N1B4_2117 +1.1 9.4 Ribonuclease PH (EC 2.7.7.56) compare
Pf1N1B4_1465 +1.0 8.8 Carbamoyltransferase in large core OS assembly cluster compare
Pf1N1B4_2441 +1.0 5.7 Electron transfer flavoprotein, alpha subunit compare
Pf1N1B4_1458 +1.0 7.0 Probable transcription regulator Mig-14 compare
Pf1N1B4_1457 +1.0 7.7 Hypothetical protein FIG015671 in large core OS assembly cluster compare
Pf1N1B4_2440 +1.0 6.4 Predicted L-lactate dehydrogenase, Iron-sulfur cluster-binding subunit YkgF compare
Pf1N1B4_3206 +1.0 2.9 Short chain dehydrogenase compare
Pf1N1B4_5924 +1.0 6.7 hypothetical protein compare
Pf1N1B4_1637 +1.0 2.8 FIG00954739: hypothetical protein compare
Pf1N1B4_483 +0.9 2.5 Metal-dependent hydrolase related to alanyl-tRNA synthetase compare
Pf1N1B4_2026 +0.8 3.6 Transcriptional regulator compare
Pf1N1B4_5926 +0.8 6.2 hypothetical protein compare
Pf1N1B4_5353 +0.8 3.9 hypothetical protein compare
Pf1N1B4_1461 +0.8 3.7 Protein fixF compare
Pf1N1B4_5055 +0.8 4.0 (R)-3-hydroxydecanoyl-ACP:CoA transacylase PhaG (3-hydroxyacyl-CoA-acyl carrier protein transferase) (EC 2.4.1.-) compare
Pf1N1B4_292 +0.8 7.7 ErfK/YbiS/YcfS/YnhG family protein compare
Pf1N1B4_3975 +0.8 2.7 Transcriptional regulator, IclR family compare
Pf1N1B4_1052 +0.8 2.6 FKBP-type peptidyl-prolyl cis-trans isomerase SlyD (EC 5.2.1.8) compare
Pf1N1B4_5644 +0.8 4.0 amino acid ABC transporter, permease protein compare
Pf1N1B4_801 +0.8 5.5 D-serine/D-alanine/glycine transporter compare
Pf1N1B4_5365 +0.8 2.0 hypothetical protein compare
Pf1N1B4_287 +0.8 5.3 Possible periplasmic thiredoxin compare
Pf1N1B4_288 +0.8 5.6 FIG00958794: hypothetical protein compare
Pf1N1B4_2024 +0.7 5.8 sodium/alanine transporter compare
Pf1N1B4_3976 +0.7 4.6 Transmembrane regulator protein PrtR compare
Pf1N1B4_35 +0.7 1.8 hypothetical protein compare
Pf1N1B4_4518 +0.7 2.7 Possible pyrimidine permease in reductive pathway compare
Pf1N1B4_1505 +0.7 4.3 FIG017861: hypothetical protein compare
Pf1N1B4_3514 +0.7 6.0 Sensor histidine kinase/response regulator compare
Pf1N1B4_286 +0.7 5.1 2-dehydropantoate 2-reductase (EC 1.1.1.169) compare
Pf1N1B4_4017 +0.7 1.6 DNA-binding heavy metal response regulator compare
Pf1N1B4_756 +0.7 2.1 hypothetical protein compare
Pf1N1B4_5342 +0.7 2.0 hypothetical protein compare
Pf1N1B4_5283 +0.7 1.5 hypothetical protein compare
Pf1N1B4_740 +0.7 2.4 Leucyl-tRNA synthetase compare
Pf1N1B4_608 +0.7 2.4 Heme oxygenase HemO, associated with heme uptake compare
Pf1N1B4_1874 +0.7 2.6 FIG00953549: hypothetical protein compare
Pf1N1B4_2382 +0.7 5.2 ATP-dependent RNA helicase RhlE compare
Pf1N1B4_2381 +0.7 5.6 Protein yceI precursor compare
Pf1N1B4_4931 +0.7 1.9 Glutarate-semialdehyde dehydrogenase (EC 1.2.1.20); Succinate-semialdehyde dehydrogenase [NADP+] (EC 1.2.1.79) compare
Pf1N1B4_676 +0.6 2.6 FIG00953991: hypothetical protein compare
Pf1N1B4_5355 +0.6 4.0 hypothetical protein compare
Pf1N1B4_4602 +0.6 2.1 Transcriptional regulators, LysR family compare
Pf1N1B4_2422 +0.6 4.5 L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1) compare
Pf1N1B4_3911 +0.6 2.9 Predicted transcriptional regulator for fatty acid degradation FadQ, TetR family compare
Pf1N1B4_291 +0.6 4.4 Major outer membrane lipoprotein I compare
Pf1N1B4_2029 +0.6 2.3 FIG00953722: hypothetical protein compare
Pf1N1B4_5854 +0.6 2.9 FIG00960144: hypothetical protein compare
Pf1N1B4_290 +0.6 6.0 Glutamate synthase [NADPH] large chain (EC 1.4.1.13) compare
Pf1N1B4_2442 +0.6 2.3 Electron transfer flavoprotein, beta subunit compare
Pf1N1B4_2438 +0.6 3.4 FIG00953113: hypothetical protein compare
Pf1N1B4_3516 +0.6 5.0 TonB-dependent receptor; Outer membrane receptor for ferrienterochelin and colicins compare
Pf1N1B4_1691 +0.6 1.6 hypothetical protein compare
Pf1N1B4_78 +0.6 2.1 FIG00956937: hypothetical protein compare
Pf1N1B4_3985 +0.6 1.8 Methylglutaconyl-CoA hydratase (EC 4.2.1.18) compare
Pf1N1B4_337 +0.6 3.6 O-antigen export system permease protein RfbD compare
Pf1N1B4_2380 +0.6 5.2 Cytochrome B561 compare
Pf1N1B4_3590 +0.6 4.4 hypothetical protein compare
Pf1N1B4_3437 +0.6 3.6 Arginine pathway regulatory protein ArgR, repressor of arg regulon compare
Pf1N1B4_3591 +0.6 4.4 3-oxoacyl-[acyl-carrier-protein] synthase, KASIII (EC 2.3.1.41) compare
Pf1N1B4_1011 +0.6 1.9 ribosomal subunit interface protein, putative compare
Pf1N1B4_3515 +0.6 2.7 FIG00953467: hypothetical protein compare
Pf1N1B4_336 +0.6 5.2 Teichoic acid export ATP-binding protein TagH (EC 3.6.3.40) compare
Pf1N1B4_4587 +0.6 1.6 probable dioxygenase compare
Pf1N1B4_1555 +0.6 4.7 Polyhydroxyalkanoic acid synthase compare
Pf1N1B4_1639 +0.6 4.4 transport permease protein of gamma-aminobutyrate compare
Pf1N1B4_3906 +0.6 2.2 DNA topoisomerase I (EC 5.99.1.2) compare
Pf1N1B4_5925 +0.6 4.4 hypothetical protein compare
Pf1N1B4_72 +0.6 1.2 Transcriptional regulator, LysR family compare
Pf1N1B4_2792 +0.6 2.8 FIG00953454: hypothetical protein compare
Pf1N1B4_2235 +0.6 4.5 Glycine cleavage system H protein compare
Pf1N1B4_846 +0.6 1.5 hypothetical protein compare
Pf1N1B4_5860 +0.6 1.0 Two-component response regulator compare
Pf1N1B4_651 +0.6 3.9 2-dehydropantoate 2-reductase (EC 1.1.1.169) compare
Pf1N1B4_1040 +0.6 3.1 FolM Alternative dihydrofolate reductase 1 compare
Pf1N1B4_4717 +0.6 1.5 Agmatinase (EC 3.5.3.11) compare
Pf1N1B4_3899 +0.5 2.3 COG0488: ATPase components of ABC transporters with duplicated ATPase domains compare
Pf1N1B4_1008 +0.5 1.6 FIG006045: Sigma factor, ECF subfamily compare
Pf1N1B4_1887 +0.5 2.5 probable exported protein YPO0432 compare
Pf1N1B4_3955 +0.5 2.5 2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34) compare
Pf1N1B4_3149 +0.5 1.9 hypothetical protein compare
Pf1N1B4_2684 +0.5 1.5 Uncharacterized protein, similar to the N-terminal domain of Lon protease compare
Pf1N1B4_5045 +0.5 2.3 Cytochrome oxidase biogenesis protein Sco1/SenC/PrrC, putative copper metallochaperone compare
Pf1N1B4_284 +0.5 4.2 Universal stress protein family compare
Pf1N1B4_5575 +0.5 1.7 hypothetical protein compare
Pf1N1B4_2544 +0.5 4.1 Phosphoglycolate phosphatase (EC 3.1.3.18) compare
Pf1N1B4_5618 +0.5 2.3 Excinuclease ABC subunit C compare
Pf1N1B4_3242 +0.5 2.1 GCN5-related N-acetyltransferase compare
Pf1N1B4_883 +0.5 1.0 Hypothetical nudix hydrolase YeaB compare
Pf1N1B4_5509 +0.5 1.1 hypothetical protein compare
Pf1N1B4_1528 +0.5 1.3 Tryptophan synthase beta chain like (EC 4.2.1.20) compare
Pf1N1B4_1460 +0.5 4.3 Lipid A core - O-antigen ligase and related enzymes compare
Pf1N1B4_668 +0.5 3.4 Exodeoxyribonuclease I (EC 3.1.11.1) compare
Pf1N1B4_2047 +0.5 2.2 Chorismate--pyruvate lyase (EC 4.1.3.40) compare
Pf1N1B4_507 +0.5 1.4 FIG00956448: hypothetical protein compare
Pf1N1B4_2668 +0.5 4.1 DedA protein compare
Pf1N1B4_3928 +0.5 2.4 FIG00955661: hypothetical protein compare
Pf1N1B4_5939 +0.5 2.3 dTDP-glucose 4,6-dehydratase (EC 4.2.1.46) compare
Pf1N1B4_4218 +0.5 1.9 Multidrug resistance protein A compare
Pf1N1B4_4668 +0.5 2.1 Heavy metal RND efflux outer membrane protein, CzcC family compare
Pf1N1B4_4657 +0.5 1.8 Oxidoreductase probably involved in sulfite reduction compare
Pf1N1B4_2734 +0.5 1.2 Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system compare
Pf1N1B4_3345 +0.5 4.0 Ribonuclease D (EC 3.1.26.3) compare
Pf1N1B4_4111 +0.5 1.7 Panthothenate synthetase compare
Pf1N1B4_1423 +0.5 3.4 Peptidase C14, caspase catalytic subunit p20 compare
Pf1N1B4_3416 +0.5 2.5 Outer membrane protein romA compare
Pf1N1B4_3589 +0.5 3.3 N-acetylneuraminate synthase (EC 2.5.1.56) compare
Pf1N1B4_3638 +0.5 1.9 FIG00955434: hypothetical protein compare
Pf1N1B4_3012 +0.5 2.2 Thiol:disulfide interchange protein DsbC compare
Pf1N1B4_5354 +0.5 1.7 hypothetical protein compare
Pf1N1B4_1389 +0.5 1.7 Lysine decarboxylase family compare
Pf1N1B4_2825 +0.5 2.3 EF hand domain protein compare
Pf1N1B4_114 +0.5 2.0 Diaminopimelate epimerase (EC 5.1.1.7) compare
Pf1N1B4_4054 +0.5 2.7 ATP-dependent Clp protease adaptor protein ClpS compare
Pf1N1B4_3023 +0.5 1.3 hypothetical protein compare
Pf1N1B4_1159 +0.5 1.7 tRNA pseudouridine synthase B (EC 4.2.1.70) compare
Pf1N1B4_2143 +0.5 4.1 Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system compare
Pf1N1B4_5269 +0.5 1.7 hypothetical protein compare
Pf1N1B4_3435 +0.5 1.2 ABC transporter for L-Arginine and L-Citrulline, ATPase component (from data) compare
Pf1N1B4_3433 +0.5 3.4 ABC transporter for L-Arginine and L-Citrulline, permease component 2 (from data) compare
Pf1N1B4_3938 +0.5 1.8 Integral membrane protein TerC compare
Pf1N1B4_384 +0.5 1.1 proteinase inhibitor compare
Pf1N1B4_3889 +0.5 1.5 hypothetical protein compare
Pf1N1B4_959 +0.5 4.4 Stringent starvation protein A compare
Pf1N1B4_5628 +0.5 1.7 Oligopeptide transport system permease protein OppC (TC 3.A.1.5.1) compare
Pf1N1B4_1542 +0.5 1.8 Type IV pilus biogenesis protein PilN compare
Pf1N1B4_4397 +0.5 1.5 Succinylglutamate desuccinylase/aspartoacylase compare
Pf1N1B4_4687 +0.5 3.0 Glycogen synthase, ADP-glucose transglucosylase (EC 2.4.1.21) compare
Pf1N1B4_3866 +0.5 0.9 Adenine phosphoribosyltransferase (EC 2.4.2.7) compare
Pf1N1B4_753 +0.5 1.9 hypothetical protein compare
Pf1N1B4_3440 +0.5 3.4 Succinylornithine transaminase (EC 2.6.1.81) (from data) compare
Pf1N1B4_4828 +0.5 1.7 hypothetical protein compare
Pf1N1B4_285 +0.5 3.6 5'-nucleotidase (EC 3.1.3.5) compare
Pf1N1B4_3191 +0.5 1.1 FIG00953505: hypothetical protein compare
Pf1N1B4_2714 +0.5 1.9 FOG: TPR repeat, SEL1 subfamily compare
Pf1N1B4_2307 +0.5 2.6 LSU m5C1962 methyltransferase RlmI compare
Pf1N1B4_866 +0.5 0.6 Iron-sulfur cluster regulator IscR compare
Pf1N1B4_3134 +0.5 1.1 probable tail fiber assembly-like protein compare
Pf1N1B4_769 +0.5 2.8 Aerobic glycerol-3-phosphate dehydrogenase (EC 1.1.5.3) compare
Pf1N1B4_1570 +0.5 2.2 Ribosomal RNA small subunit methyltransferase E (EC 2.1.1.-) compare
Pf1N1B4_4799 +0.5 1.5 Multidrug resistance protein B compare
Pf1N1B4_3093 +0.5 1.3 Outer membrane protein H precursor compare
Pf1N1B4_1419 +0.5 2.4 putative membrane protein compare
Pf1N1B4_5087 +0.5 2.3 Glycosyl transferase, group 1 family protein compare
Pf1N1B4_4280 +0.5 1.6 Myo-inositol 2-dehydrogenase (EC 1.1.1.18) compare
Pf1N1B4_4107 +0.5 1.0 Probable glutathione S-transferase (EC 2.5.1.18), YfcF homolog compare
Pf1N1B4_4661 +0.5 1.7 Cell division inhibitor SULA compare
Pf1N1B4_3791 +0.5 2.4 Protein containing domains DUF403 compare
Pf1N1B4_2352 +0.5 2.8 Glutathione synthetase (EC 6.3.2.3) compare
Pf1N1B4_4219 +0.4 1.6 FIG01074225: hypothetical protein compare
Pf1N1B4_1194 +0.4 1.6 hypothetical protein compare
Pf1N1B4_2681 +0.4 2.5 MaoC-like domain protein compare
Pf1N1B4_2350 +0.4 1.5 UPF0301 protein YqgE compare
Pf1N1B4_695 +0.4 1.4 Aldehyde dehydrogenase (EC 1.2.1.3) compare
Pf1N1B4_2122 +0.4 3.6 Phosphomannomutase (EC 5.4.2.8) / Phosphoglucomutase (EC 5.4.2.2) compare
Pf1N1B4_1464 +0.4 3.1 Glycosyl transferase compare
Pf1N1B4_3631 +0.4 1.8 Flagellar motor rotation protein MotA compare
Pf1N1B4_1705 +0.4 1.7 SAM-dependent methyltransferases compare
Pf1N1B4_669 +0.4 1.9 INTEGRAL MEMBRANE PROTEIN (Rhomboid family) compare
Pf1N1B4_189 +0.4 1.9 Short-chain dehydrogenase, associated with 2-hydroxychromene-2-carboxylate isomerase family protein compare
Pf1N1B4_3567 +0.4 3.5 Phenylalanine hydroxylase transcriptional activator PhhR compare
Pf1N1B4_34 +0.4 1.3 Xanthine permease compare
Pf1N1B4_482 +0.4 2.4 Transcriptional regulator, LysR family compare
Pf1N1B4_5829 +0.4 1.3 FIG005107: hypothetical protein compare
Pf1N1B4_4438 +0.4 1.1 Gamma-glutamyl phosphate reductase (EC 1.2.1.41) compare
Pf1N1B4_1488 +0.4 2.0 hypothetical protein compare
Pf1N1B4_1482 +0.4 1.6 hypothetical protein compare
Pf1N1B4_403 +0.4 1.2 FIG00965925: hypothetical protein compare
Pf1N1B4_5604 +0.4 1.6 Permeases of the major facilitator superfamily compare
Pf1N1B4_1701 +0.4 2.3 Methionine ABC transporter substrate-binding protein compare
Pf1N1B4_5309 +0.4 0.9 hypothetical protein compare
Pf1N1B4_2471 +0.4 1.7 putative iron-regulated membrane protein compare
Pf1N1B4_501 +0.4 1.1 Flp pilus assembly protein RcpC/CpaB compare
Pf1N1B4_4566 +0.4 1.5 Universal stress protein family 7 compare
Pf1N1B4_5496 +0.4 1.0 hypothetical protein compare
Pf1N1B4_3818 +0.4 1.4 Propionate catabolism operon transcriptional regulator of GntR family [predicted] compare
Pf1N1B4_5930 +0.4 2.4 Exopolysaccharide production protein ExoZ compare
Pf1N1B4_3331 +0.4 1.5 Glycine cleavage system transcriptional antiactivator GcvR compare
Pf1N1B4_3795 +0.4 1.2 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9) compare
Pf1N1B4_4732 +0.4 1.9 Fap amyloid fiber secretin compare
Pf1N1B4_121 +0.4 2.0 Membrane fusion component of tripartite multidrug resistance system compare
Pf1N1B4_5480 +0.4 1.4 hypothetical protein compare
Pf1N1B4_1866 +0.4 1.8 Methionine ABC transporter ATP-binding protein compare
Pf1N1B4_4705 +0.4 1.4 Delta 1-piperideine-2-carboxylate reductase (EC 1.5.1.21) / Delta 1-pyrroline-2-carboxylate reductase (EC 1.5.1.1) compare


Specific Phenotypes

For 7 genes in this experiment

For nitrogen source L-Serine in Pseudomonas fluorescens FW300-N1B4

For nitrogen source L-Serine across organisms