Experiment set5IT047 for Pseudomonas fluorescens FW300-N1B4

Compare to:

L-Glutamic acid monopotassium salt monohydrate nitrogen source

Group: nitrogen source
Media: RCH2_defined_Glucose_noNitrogen + L-Glutamic acid monopotassium salt monohydrate (20 mM), pH=7
Culturing: pseudo1_N1B4_ML1, tube, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 6.6 generations
By: Jayashree on 11/19/2014
Media components: 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 20 mM D-Glucose, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 1 genes in this experiment

For nitrogen source L-Glutamic acid monopotassium salt monohydrate in Pseudomonas fluorescens FW300-N1B4

For nitrogen source L-Glutamic acid monopotassium salt monohydrate across organisms

SEED Subsystems

Subsystem #Specific
Cobalamin synthesis 1
Coenzyme B12 biosynthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation) 13 13 1
cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion) 15 13 1
adenosylcobalamin biosynthesis II (aerobic) 33 31 1
adenosylcobalamin biosynthesis I (anaerobic) 36 29 1