Experiment set5IT046 for Pseudomonas fluorescens FW300-N2E2

LB with Gjerstad humics 0.5 M

Group: stress
Media: LB + Gjerstad humics (0.5 M), pH=7
Culturing: pseudo6_N2E2_ML5, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=500 rpm
By: Hans_Hualan on 2/2/2016
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 3 genes in this experiment

For stress Gjerstad humics in Pseudomonas fluorescens FW300-N2E2

For stress Gjerstad humics across organisms

SEED Subsystems

Subsystem	#Specific
Carboxysome	1
Cyanate hydrolysis	1
Methionine Salvage	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Methionine metabolism
• Nitrogen metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
3-methylthiopropanoate biosynthesis	1	1	1
CO2 fixation into oxaloacetate (anaplerotic)	2	2	1
cyanate degradation	3	3	1
C4 photosynthetic carbon assimilation cycle, NADP-ME type	7	4	1
S-methyl-5-thio-α-D-ribose 1-phosphate degradation I	7	2	1
C4 photosynthetic carbon assimilation cycle, NAD-ME type	11	6	1
L-methionine salvage cycle III	11	5	1
L-methionine salvage cycle II (plants)	11	3	1
L-methionine salvage cycle I (bacteria and plants)	12	4	1
glyoxylate assimilation	13	6	1
3-hydroxypropanoate cycle	13	6	1
C4 photosynthetic carbon assimilation cycle, PEPCK type	14	9	1
3-hydroxypropanoate/4-hydroxybutanate cycle	18	9	1
gluconeogenesis II (Methanobacterium thermoautotrophicum)	18	9	1
superpathway of the 3-hydroxypropanoate cycle	18	7	1
Methanobacterium thermoautotrophicum biosynthetic metabolism	56	21	1