Experiment set5IT046 for Pseudomonas fluorescens SBW25-INTG

Growth at 34C

Group: temperature
Media: soilextract_PNNL_Prosser_PlotA_B_20191220 + D-Glucose (10 mM) + Ammonium chloride (10 mM)
Culturing: PseudoSBW25_INTG_ML3, 24-well transparent microplate; Multitron, Aerobic, at 34 (C)
By: Joshua Elmore on 1/6/20

Specific Phenotypes

For 15 genes in this experiment

For temperature D-Glucose in Pseudomonas fluorescens SBW25-INTG

For temperature D-Glucose across organisms

SEED Subsystems

Subsystem	#Specific
High affinity phosphate transporter and control of PHO regulon	2
Phosphate metabolism	2
ABC transporter alkylphosphonate (TC 3.A.1.9.1)	1
Alginate metabolism	1
DNA repair, bacterial	1
Mannose Metabolism	1
Peptidoglycan Biosynthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Nucleotide sugars metabolism
• Ascorbate and aldarate metabolism
• Starch and sucrose metabolism
• Glycolysis / Gluconeogenesis
• Pentose phosphate pathway
• Pentose and glucuronate interconversions
• Fructose and mannose metabolism
• Galactose metabolism
• Ubiquinone and menaquinone biosynthesis
• Streptomycin biosynthesis
• Glycosaminoglycan degradation
• Lipopolysaccharide biosynthesis

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
UDP-α-D-glucuronate biosynthesis (from UDP-glucose)	1	1	1
UDP-α-D-glucose biosynthesis	2	2	1
UDP-α-D-xylose biosynthesis	2	1	1
superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis	6	4	2
β-1,4-D-mannosyl-N-acetyl-D-glucosamine degradation	3	2	1
GDP-α-D-glucose biosynthesis	3	2	1
trehalose degradation V	3	2	1
polymyxin resistance	6	3	2
colanic acid building blocks biosynthesis	11	11	3
GDP-mannose biosynthesis	4	4	1
sucrose degradation IV (sucrose phosphorylase)	4	3	1
starch degradation V	4	3	1
glycogen biosynthesis I (from ADP-D-Glucose)	4	3	1
starch degradation III	4	2	1
dTDP-β-L-rhamnose biosynthesis	5	5	1
sucrose degradation II (sucrose synthase)	5	4	1
glucose and glucose-1-phosphate degradation	5	4	1
D-galactose degradation I (Leloir pathway)	5	3	1
glucosylglycerol biosynthesis	5	2	1
CDP-6-deoxy-D-gulose biosynthesis	5	1	1
glycogen degradation II	6	5	1
β-(1,4)-mannan degradation	7	2	1
sucrose biosynthesis II	8	6	1
glycogen degradation I	8	6	1
glycogen biosynthesis III (from α-maltose 1-phosphate)	8	3	1
sucrose biosynthesis I (from photosynthesis)	9	7	1
chitin biosynthesis	9	5	1
teichuronic acid biosynthesis (B. subtilis 168)	9	2	1
UDP-sugars interconversion	9	2	1
starch biosynthesis	10	5	1
O-antigen building blocks biosynthesis (E. coli)	11	10	1
superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis	14	9	1
streptomycin biosynthesis	18	2	1
superpathway of anaerobic sucrose degradation	19	14	1
superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis	19	5	1
superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis	33	12	1