Experiment set5IT045 for Klebsiella michiganensis M5al

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Sodium D-Lactate carbon source

Group: carbon source
Media: RCH2_defined_noCarbon + Sodium D-Lactate (20 mM), pH=7
Culturing: Koxy_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Adam on 27-Mar-17
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Growth plate: 1764 D1

Specific Phenotypes

For 12 genes in this experiment

For carbon source Sodium D-Lactate in Klebsiella michiganensis M5al

For carbon source Sodium D-Lactate across organisms

SEED Subsystems

Subsystem #Specific
Lactate utilization 3
Acetoin, butanediol metabolism 2
Branched-Chain Amino Acid Biosynthesis 2
Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate 2
DNA-binding regulatory proteins, strays 1
Entner-Doudoroff Pathway 1
Formate hydrogenase 1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria 1
Ketoisovalerate oxidoreductase 1
Methylglyoxal Metabolism 1
Murein hydrolase regulation and cell death 1
Respiratory dehydrogenases 1 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
acetate conversion to acetyl-CoA 1 1 1
acetate and ATP formation from acetyl-CoA III 1 1 1
pyruvate fermentation to (R)-lactate 1 1 1
ethanol degradation IV 3 3 2
ethanol degradation II 3 3 2
ethanol degradation III 3 2 2
pyruvate fermentation to (R)-acetoin II 2 2 1
putrescine degradation I 2 2 1
ethylene glycol degradation 2 2 1
phenylethylamine degradation I 2 2 1
phenylethylamine degradation II 2 1 1
phenylethanol degradation 2 1 1
putrescine degradation V 2 1 1
D-lactate to cytochrome bo oxidase electron transfer 2 1 1
superpathway of acetate utilization and formation 3 3 1
pyruvate fermentation to (S)-acetoin 3 3 1
L-alanine degradation II (to D-lactate) 3 3 1
pyruvate fermentation to (R)-acetoin I 3 3 1
L-isoleucine biosynthesis IV 6 4 2
L-phenylalanine degradation II (anaerobic) 3 2 1
hypotaurine degradation 3 2 1
L-isoleucine biosynthesis V 3 2 1
putrescine degradation IV 3 2 1
2-chloroacrylate degradation I 3 1 1
histamine degradation 3 1 1
vancomycin resistance I 3 1 1
styrene degradation 3 1 1
D-arabinose degradation II 4 4 1
L-valine biosynthesis 4 4 1
chitin deacetylation 4 3 1
phytol degradation 4 3 1
fatty acid α-oxidation I (plants) 4 2 1
putrescine degradation III 4 2 1
L-tryptophan degradation X (mammalian, via tryptamine) 4 1 1
superpathway of (R,R)-butanediol biosynthesis 5 5 1
pyruvate fermentation to isobutanol (engineered) 5 4 1
mitochondrial NADPH production (yeast) 5 3 1
sphingosine and sphingosine-1-phosphate metabolism 10 4 2
octane oxidation 5 2 1
2-methylcitrate cycle I 5 2 1
dopamine degradation 5 1 1
superpathway of 2,3-butanediol biosynthesis 6 5 1
L-alanine degradation VI (reductive Stickland reaction) 6 4 1
3-methyl-branched fatty acid α-oxidation 6 3 1
2-methylcitrate cycle II 6 2 1
superpathway of bitter acids biosynthesis 18 3 3
β-alanine biosynthesis II 6 1 1
adlupulone and adhumulone biosynthesis 6 1 1
alkane oxidation 6 1 1
lupulone and humulone biosynthesis 6 1 1
colupulone and cohumulone biosynthesis 6 1 1
noradrenaline and adrenaline degradation 13 4 2
superpathway of glycol metabolism and degradation 7 7 1
L-isoleucine biosynthesis I (from threonine) 7 7 1
L-isoleucine biosynthesis III 7 5 1
serotonin degradation 7 3 1
ceramide degradation by α-oxidation 7 2 1
limonene degradation IV (anaerobic) 7 1 1
superpathway of ornithine degradation 8 8 1
lactate fermentation to acetate, CO2 and hydrogen (Desulfovibrionales) 8 5 1
superpathway of NAD/NADP - NADH/NADPH interconversion (yeast) 8 5 1
L-isoleucine biosynthesis II 8 4 1
aromatic biogenic amine degradation (bacteria) 8 3 1
ceramide and sphingolipid recycling and degradation (yeast) 16 4 2
superpathway of branched chain amino acid biosynthesis 17 17 2
superpathway of fermentation (Chlamydomonas reinhardtii) 9 9 1
Entner-Doudoroff pathway II (non-phosphorylative) 9 7 1
superpathway of L-alanine fermentation (Stickland reaction) 9 7 1
reductive glycine pathway of autotrophic CO2 fixation 9 6 1
L-phenylalanine degradation IV (mammalian, via side chain) 9 5 1
cis-geranyl-CoA degradation 9 1 1
superpathway of coenzyme A biosynthesis II (plants) 10 5 1
superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation 11 11 1
superpathway of phenylethylamine degradation 11 11 1
superpathway of L-isoleucine biosynthesis I 13 13 1
superpathway of L-arginine and L-ornithine degradation 13 13 1
mixed acid fermentation 16 16 1
superpathway of glucose and xylose degradation 17 17 1
superpathway of L-threonine metabolism 18 18 1
heterolactic fermentation 18 16 1
anaerobic aromatic compound degradation (Thauera aromatica) 27 3 1
superpathway of pentose and pentitol degradation 42 21 1