Experiment set5IT043 for Pseudomonas fluorescens FW300-N2E3
outer cut, LB soft agar motility assay
Group: motilityMedia: LB + Agar (3 g/L)
Culturing: pseudo3_N2E3_ML2, Agar plate, Aerobic, at 30 (C), shaken=0 rpm, (Solid)
By: Mark on 12/17/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Specific Phenotypes
For 61 genes in this experiment
For motility Agar in Pseudomonas fluorescens FW300-N2E3
For motility Agar across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Ascorbate and aldarate metabolism
- Fatty acid metabolism
- Ubiquinone and menaquinone biosynthesis
- Oxidative phosphorylation
- Photosynthesis
- Glycine, serine and threonine metabolism
- Valine, leucine and isoleucine degradation
- beta-Alanine metabolism
- Cyanoamino acid metabolism
- Nucleotide sugars metabolism
- Glycosaminoglycan degradation
- Propanoate metabolism
- One carbon pool by folate
- Methane metabolism
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| glycine biosynthesis I | 1 | 1 | 1 |
| dTMP de novo biosynthesis (mitochondrial) | 3 | 3 | 1 |
| glycine degradation | 3 | 3 | 1 |
| superpathway of L-serine and glycine biosynthesis I | 4 | 4 | 1 |
| glycine betaine degradation II (mammalian) | 4 | 1 | 1 |
| folate polyglutamylation | 5 | 4 | 1 |
| L-leucine degradation I | 6 | 5 | 1 |
| glycine betaine degradation III | 7 | 7 | 1 |
| glycine betaine degradation I | 8 | 6 | 1 |
| folate transformations III (E. coli) | 9 | 9 | 1 |
| photorespiration I | 9 | 5 | 1 |
| photorespiration III | 9 | 5 | 1 |
| photorespiration II | 10 | 6 | 1 |
| folate transformations II (plants) | 11 | 10 | 1 |
| folate transformations I | 13 | 9 | 1 |
| formaldehyde assimilation I (serine pathway) | 13 | 6 | 1 |
| purine nucleobases degradation II (anaerobic) | 24 | 16 | 1 |