Experiment set5IT042 for Pseudomonas fluorescens FW300-N2E2

Compare to:

LB with Cadmium chloride hemipentahydrate 0.00005 M

Group: stress
Media: LB + Cadmium chloride hemipentahydrate (5e-05 M), pH=7
Culturing: pseudo6_N2E2_ML5, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=500 rpm
By: Hans_Hualan on 2/2/2016
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 3 genes in this experiment

For stress Cadmium chloride hemipentahydrate in Pseudomonas fluorescens FW300-N2E2

For stress Cadmium chloride hemipentahydrate across organisms

SEED Subsystems

Subsystem #Specific
Glycine and Serine Utilization 2
Cysteine Biosynthesis 1
Glycine cleavage system 1
Methionine Biosynthesis 1
Photorespiration (oxidative C2 cycle) 1
Pyruvate Alanine Serine Interconversions 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-serine degradation 3 3 3
D-serine degradation 3 3 2
L-cysteine degradation II 3 3 2
L-tryptophan degradation II (via pyruvate) 3 2 2
L-cysteine biosynthesis I 2 2 1
glycine betaine degradation III 7 7 3
felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis 5 2 2
glycine betaine degradation I 8 6 3
glycine cleavage 3 3 1
glycine degradation 3 3 1
glycine biosynthesis II 3 3 1
L-methionine biosynthesis II 6 5 2
L-mimosine degradation 8 4 2
glutathione-mediated detoxification I 8 3 2
seleno-amino acid biosynthesis (plants) 5 3 1
hydrogen sulfide biosynthesis II (mammalian) 6 1 1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II 15 13 2
purine nucleobases degradation II (anaerobic) 24 16 3
superpathway of sulfate assimilation and cysteine biosynthesis 9 9 1
superpathway of seleno-compound metabolism 19 6 1