Experiment set5IT038 for Pseudomonas fluorescens SBW25-INTG

N-Acetyl-D-Glucosamine carbon source; with MOPS

Group: carbon source
Media: soilextract_PNNL_Prosser_PlotA_B_20191220 + N-Acetyl-D-Glucosamine (10 mM) + Ammonium chloride (10 mM) + 3-(N-morpholino)propanesulfonic acid (40 mM), pH=7
Culturing: PseudoSBW25_INTG_ML3, 96 deep-well microplate; 1 mL volume, Aerobic, at 30 (C)
By: Joshua Elmore on 1/6/20

Specific Phenotypes

For 16 genes in this experiment

For carbon source N-Acetyl-D-Glucosamine in Pseudomonas fluorescens SBW25-INTG

For carbon source N-Acetyl-D-Glucosamine across organisms

SEED Subsystems

Subsystem	#Specific
Chitin and N-acetylglucosamine utilization	3
Folate Biosynthesis	2
Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate	2
UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis	2
Fructose and Mannose Inducible PTS	1
Fructose utilization	1
Heat shock dnaK gene cluster extended	1
Mannitol Utilization	1
Ribosome biogenesis bacterial	1
Sialic Acid Metabolism	1
Trehalose Uptake and Utilization	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Nucleotide sugars metabolism
• Aminosugars metabolism
• Glycolysis / Gluconeogenesis
• Galactose metabolism
• Tryptophan metabolism
• Pentose and glucuronate interconversions
• Fructose and mannose metabolism
• Ascorbate and aldarate metabolism
• Biosynthesis of steroids
• Glutamate metabolism
• Starch and sucrose metabolism
• Lipopolysaccharide biosynthesis
• One carbon pool by folate
• Methane metabolism
• Folate biosynthesis
• Carotenoid biosynthesis - General
• Biosynthesis of siderophore group nonribosomal peptides
• Biosynthesis of phenylpropanoids
• Biosynthesis of alkaloids derived from terpenoid and polyketide

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
N-acetylglucosamine degradation I	2	2	2
N-acetylglucosamine degradation II	3	2	2
tetrahydrofolate biosynthesis I	3	3	1
dTMP de novo biosynthesis (mitochondrial)	3	3	1
superpathway of N-acetylglucosamine, N-acetylmannosamine and N-acetylneuraminate degradation	6	2	2
oxalate degradation II	3	1	1
D-galactosamine and N-acetyl-D-galactosamine degradation	4	2	1
tetrahydromonapterin biosynthesis	4	2	1
chitin derivatives degradation	8	2	2
UDP-N-acetyl-D-glucosamine biosynthesis I	5	5	1
N-acetyl-D-galactosamine degradation	5	2	1
UDP-N-acetyl-D-glucosamine biosynthesis II	6	4	1
UDP-N-acetyl-D-galactosamine biosynthesis III	6	2	1
UDP-N-acetyl-D-galactosamine biosynthesis II	7	5	1
chitin degradation I (archaea)	7	1	1
folate transformations III (E. coli)	9	9	1
chitin biosynthesis	9	5	1
superpathway of tetrahydrofolate biosynthesis	10	8	1
CMP-legionaminate biosynthesis I	10	2	1
O-antigen building blocks biosynthesis (E. coli)	11	10	1
folate transformations II (plants)	11	10	1
superpathway of N-acetylneuraminate degradation	22	14	2
superpathway of tetrahydrofolate biosynthesis and salvage	12	10	1
peptidoglycan recycling I	14	11	1
superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis	24	9	1
superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis	33	12	1
superpathway of chorismate metabolism	59	42	1