Experiment set5IT037 for Pseudomonas fluorescens FW300-N2E2
LB with Sodium selenite pentahydrate 0.0025 M
Group: stressMedia: LB + Sodium selenite pentahydrate (0.0025 M), pH=7
Culturing: pseudo6_N2E2_ML5, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=500 rpm
By: Hans_Hualan on 2/2/2016
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Specific Phenotypes
For 6 genes in this experiment
For stress Sodium selenite pentahydrate in Pseudomonas fluorescens FW300-N2E2
For stress Sodium selenite pentahydrate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Cysteine Biosynthesis | 1 |
| Glycerol and Glycerol-3-phosphate Uptake and Utilization | 1 |
| Methionine Biosynthesis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| thiosulfate disproportionation IV (rhodanese) | 1 | 1 | 1 |
| L-cysteine biosynthesis I | 2 | 2 | 1 |
| L-cysteine degradation III | 2 | 1 | 1 |
| seleno-amino acid biosynthesis (plants) | 5 | 3 | 1 |
| sulfide oxidation IV (mitochondria) | 5 | 2 | 1 |
| hydrogen sulfide biosynthesis II (mammalian) | 6 | 1 | 1 |
| superpathway of sulfate assimilation and cysteine biosynthesis | 9 | 9 | 1 |
| superpathway of seleno-compound metabolism | 19 | 6 | 1 |