Experiment set5IT035 for Klebsiella michiganensis M5al

D-Raffinose pentahydrate carbon source

Group: carbon source
Media: RCH2_defined_noCarbon + D-Raffinose pentahydrate (20 mM), pH=7
Culturing: Koxy_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Adam on 27-Mar-17
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Growth plate: 1764 B2

Specific Phenotypes

For 9 genes in this experiment

For carbon source D-Raffinose pentahydrate in Klebsiella michiganensis M5al

For carbon source D-Raffinose pentahydrate across organisms

SEED Subsystems

Subsystem	#Specific
Colanic acid biosynthesis	1
Fructose utilization	1
Rhamnose containing glycans	1
Teichoic and lipoteichoic acids biosynthesis	1
Trehalose Uptake and Utilization	1
tRNA processing	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Galactose metabolism
• Starch and sucrose metabolism
• Nucleotide sugars metabolism
• Glycolysis / Gluconeogenesis
• Pentose and glucuronate interconversions
• Fructose and mannose metabolism
• Ascorbate and aldarate metabolism
• Aminosugars metabolism
• T cell receptor signaling pathway

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
UDP-α-D-glucose biosynthesis	2	2	1
sucrose degradation II (sucrose synthase)	5	4	1
superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis	6	4	1
stachyose degradation	7	6	1
sucrose biosynthesis II	8	6	1
sucrose biosynthesis I (from photosynthesis)	9	7	1
tRNA processing	10	10	1
colanic acid building blocks biosynthesis	11	5	1
type I lipoteichoic acid biosynthesis (S. aureus)	17	5	1
superpathway of anaerobic sucrose degradation	19	18	1