Experiment set5IT033 for Klebsiella michiganensis M5al

L-Sorbose carbon source

Group: carbon source
Media: RCH2_defined_noCarbon + L-(-)-sorbose (20 mM), pH=7
Culturing: Koxy_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Adam on 27-Mar-17
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Growth plate: 1764 A6

Specific Phenotypes

For 12 genes in this experiment

For carbon source L-(-)-sorbose in Klebsiella michiganensis M5al

For carbon source L-(-)-sorbose across organisms

SEED Subsystems

Subsystem	#Specific
D-Sorbitol(D-Glucitol) and L-Sorbose Utilization	5
Glycerol and Glycerol-3-phosphate Uptake and Utilization	2
Glycerol fermenation to 1,3-propanediol	1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria	1
MLST	1
Phosphate metabolism	1
Sugar-phosphate stress regulation	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Fructose and mannose metabolism
• Ascorbate and aldarate metabolism
• Nucleotide sugars metabolism
• Aminosugars metabolism
• Glycolysis / Gluconeogenesis
• Galactose metabolism
• C21-Steroid hormone metabolism
• Glycine, serine and threonine metabolism
• Bisphenol A degradation
• Starch and sucrose metabolism
• Polyketide sugar unit biosynthesis
• Glycerolipid metabolism
• Linoleic acid metabolism
• Tetrachloroethene degradation
• Benzoate degradation via CoA ligation
• Butanoate metabolism
• Nicotinate and nicotinamide metabolism
• Retinol metabolism
• Alkaloid biosynthesis I
• Insect hormone biosynthesis
• Biosynthesis of unsaturated fatty acids
• Biosynthesis of type II polyketide products
• Biosynthesis of alkaloids derived from shikimate pathway

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-sorbose degradation	2	2	2
D-sorbitol degradation II	1	1	1
glycerol degradation I	3	3	2
glycerol and glycerophosphodiester degradation	4	4	2
NAD phosphorylation and transhydrogenation	2	2	1
D-mannose degradation I	2	2	1
arsenate detoxification III	2	2	1
NAD phosphorylation and dephosphorylation	3	3	1
D-glucosaminate degradation	3	2	1
arsenic detoxification (plants)	6	5	1
NAD(P)/NADPH interconversion	6	4	1
arsenic detoxification (yeast)	12	4	1
glycolysis I (from glucose 6-phosphate)	13	13	1
superpathway of glycolysis and the Entner-Doudoroff pathway	17	17	1
arsenic detoxification (mammals)	17	8	1
superpathway of hexitol degradation (bacteria)	18	18	1
hexitol fermentation to lactate, formate, ethanol and acetate	19	19	1
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass	26	25	1