Experiment set5IT029 for Pseudomonas fluorescens FW300-N2E3

N-Acetyl-D-Glucosamine nitrogen source

Group: nitrogen source
Media: RCH2_defined_Glucose_noNitrogen + N-Acetyl-D-Glucosamine (5 mM), pH=7
Culturing: pseudo3_N2E3_ML2, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=750 rpm
By: Mark on 12/17/2015
Media components: 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 20 mM D-Glucose, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 5 genes in this experiment

For nitrogen source N-Acetyl-D-Glucosamine in Pseudomonas fluorescens FW300-N2E3

For nitrogen source N-Acetyl-D-Glucosamine across organisms

SEED Subsystems

Subsystem	#Specific
Chitin and N-acetylglucosamine utilization	3
Sialic Acid Metabolism	2
UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis	2
Fructose and Mannose Inducible PTS	1
Fructose utilization	1
Mannitol Utilization	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Nucleotide sugars metabolism
• Aminosugars metabolism
• Glycolysis / Gluconeogenesis
• Galactose metabolism
• Fructose and mannose metabolism
• Ascorbate and aldarate metabolism
• Starch and sucrose metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
N-acetylglucosamine degradation I	2	2	2
N-acetylglucosamine degradation II	3	2	2
superpathway of N-acetylglucosamine, N-acetylmannosamine and N-acetylneuraminate degradation	6	2	2
D-galactosamine and N-acetyl-D-galactosamine degradation	4	2	1
chitin derivatives degradation	8	2	2
N-acetyl-D-galactosamine degradation	5	2	1
UDP-N-acetyl-D-galactosamine biosynthesis II	7	5	1
chitin degradation I (archaea)	7	2	1
superpathway of N-acetylneuraminate degradation	22	15	2
peptidoglycan recycling I	14	11	1