Experiment set5IT027 for Pseudomonas fluorescens FW300-N1B4

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Minimal media pH9

200 most important genes:

  gene name fitness t score description  
Pf1N1B4_339 -6.4 -3.1 UDP-glucose 4-epimerase (EC 5.1.3.2) compare
Pf1N1B4_15 -5.3 -3.6 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33) compare
Pf1N1B4_619 -5.0 -3.1 Sigma-54 dependent transcriptional regulator/sensory box protein compare
Pf1N1B4_3168 -4.6 -3.2 AmpG permease compare
Pf1N1B4_630 -4.5 -6.2 TPR repeat containing exported protein; Putative periplasmic protein contains a protein prenylyltransferase domain compare
Pf1N1B4_626 -4.5 -11.2 Quinolinate synthetase (EC 2.5.1.72) compare
Pf1N1B4_2548 -4.5 -7.5 Anthranilate phosphoribosyltransferase (EC 2.4.2.18) compare
Pf1N1B4_1408 -4.3 -6.5 ATP phosphoribosyltransferase regulatory subunit (EC 2.4.2.17) compare
Pf1N1B4_1153 -4.3 -5.6 Quinolinate phosphoribosyltransferase [decarboxylating] (EC 2.4.2.19) compare
Pf1N1B4_5665 -4.2 -6.9 Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2) compare
Pf1N1B4_2338 -4.1 -9.8 Homoserine O-acetyltransferase (EC 2.3.1.31) compare
Pf1N1B4_596 -4.0 -5.5 Glucose ABC transport system, periplasmic sugar-binding protein compare
Pf1N1B4_1533 -4.0 -11.5 Glutamate synthase [NADPH] small chain (EC 1.4.1.13) compare
Pf1N1B4_3997 -4.0 -6.6 Uncharacterized protein YtfM precursor compare
Pf1N1B4_2880 -4.0 -15.4 Ketol-acid reductoisomerase (EC 1.1.1.86) compare
Pf1N1B4_5666 -4.0 -4.7 Oxidoreductase probably involved in sulfite reduction compare
Pf1N1B4_4065 -3.9 -10.4 Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107) compare
Pf1N1B4_3998 -3.9 -6.9 putative exported protein compare
Pf1N1B4_6 -3.8 -5.9 Phosphoribosylanthranilate isomerase (EC 5.3.1.24) compare
Pf1N1B4_600 -3.7 -7.6 Glucokinase (EC 2.7.1.2) compare
Pf1N1B4_4299 -3.7 -4.6 sensor histidine kinase compare
Pf1N1B4_2208 -3.7 -8.4 Polyphosphate kinase (EC 2.7.4.1) compare
Pf1N1B4_2284 -3.7 -7.5 FIG001592: Phosphocarrier protein kinase/phosphorylase, nitrogen regulation associated compare
Pf1N1B4_1534 -3.7 -22.3 Glutamate synthase [NADPH] large chain (EC 1.4.1.13) compare
Pf1N1B4_2545 -3.7 -17.9 Anthranilate synthase, aminase component (EC 4.1.3.27) compare
Pf1N1B4_3696 -3.7 -15.5 FIG00955360: hypothetical protein compare
Pf1N1B4_2144 -3.6 -13.2 D-amino acid dehydrogenase small subunit (EC 1.4.99.1) compare
Pf1N1B4_2489 -3.6 -6.9 Biotin synthesis protein BioH compare
Pf1N1B4_5827 -3.5 -3.0 Na(+) H(+) antiporter subunit G compare
Pf1N1B4_1624 -3.5 -9.1 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data) compare
Pf1N1B4_1831 -3.5 -14.9 Tryptophan synthase beta chain (EC 4.2.1.20) compare
Pf1N1B4_1307 -3.5 -3.3 3-dehydroquinate dehydratase II (EC 4.2.1.10) compare
Pf1N1B4_1590 -3.4 -7.3 Histidine utilization repressor compare
Pf1N1B4_2009 -3.4 -7.3 LysR family transcriptional regulator PA5437 compare
Pf1N1B4_1610 -3.4 -6.2 Nitrogen regulation protein NtrB (EC 2.7.13.3) compare
Pf1N1B4_5824 -3.4 -5.8 Na(+) H(+) antiporter subunit D compare
Pf1N1B4_2491 -3.4 -4.6 Dethiobiotin synthetase (EC 6.3.3.3) compare
Pf1N1B4_1565 -3.3 -5.4 Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19) compare
Pf1N1B4_1627 -3.2 -5.8 Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19) compare
Pf1N1B4_742 -3.2 -11.3 Argininosuccinate synthase (EC 6.3.4.5) compare
Pf1N1B4_350 -3.2 -8.0 Chorismate mutase I (EC 5.4.99.5) / Prephenate dehydratase (EC 4.2.1.51) compare
Pf1N1B4_5822 -3.2 -7.2 Na(+) H(+) antiporter subunit A / Na(+) H(+) antiporter subunit B compare
Pf1N1B4_3468 -3.1 -2.7 Negative regulator of flagellin synthesis FlgM compare
Pf1N1B4_322 -3.1 -3.7 nucleotide sugar epimerase/dehydratase WbpM compare
Pf1N1B4_58 -3.1 -7.2 Transaldolase (EC 2.2.1.2) compare
Pf1N1B4_2272 -3.1 -6.0 D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95) compare
Pf1N1B4_2010 -3.0 -7.9 Pyruvate carboxyl transferase subunit A (EC 6.4.1.1) compare
Pf1N1B4_2879 -3.0 -7.7 Acetolactate synthase small subunit (EC 2.2.1.6) compare
Pf1N1B4_2486 -3.0 -3.0 hypothetical protein compare
Pf1N1B4_2303 -3.0 -12.6 Dihydroxy-acid dehydratase (EC 4.2.1.9) compare
Pf1N1B4_1057 -3.0 -3.7 Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4) / Adenylylsulfate kinase (EC 2.7.1.25) compare
Pf1N1B4_3506 -2.9 -8.9 Cysteine synthase B (EC 2.5.1.47) compare
Pf1N1B4_1830 -2.9 -9.4 Tryptophan synthase alpha chain (EC 4.2.1.20) compare
Pf1N1B4_2718 -2.9 -8.1 Hydroxymethylpyrimidine phosphate kinase ThiD (EC 2.7.4.7) compare
Pf1N1B4_595 -2.9 -10.3 Glucose ABC transport system, inner membrane component 1 compare
Pf1N1B4_2695 -2.9 -2.7 Rare lipoprotein A precursor compare
Pf1N1B4_593 -2.9 -7.8 Glucose ABC transporter, ATP-binding subunit (EC 3.6.3.-) compare
Pf1N1B4_1315 -2.9 -8.5 IMP cyclohydrolase (EC 3.5.4.10) / Phosphoribosylaminoimidazolecarboxamide formyltransferase (EC 2.1.2.3) compare
Pf1N1B4_2878 -2.9 -10.3 Acetolactate synthase large subunit (EC 2.2.1.6) compare
Pf1N1B4_594 -2.8 -8.4 Glucose ABC transport system, inner membrane component 2 compare
Pf1N1B4_2549 -2.8 -8.1 Indole-3-glycerol phosphate synthase (EC 4.1.1.48) compare
Pf1N1B4_2177 -2.8 -1.4 FIG00953342: hypothetical protein compare
Pf1N1B4_2858 -2.8 -12.8 Two-component sensor CbrB: intrcellular carbon:nitrogen balance compare
Pf1N1B4_188 -2.8 -2.7 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf1N1B4_3328 -2.8 -2.6 Rhodanese-like domain protein compare
Pf1N1B4_2853 -2.8 -3.1 Pantoate--beta-alanine ligase (EC 6.3.2.1) compare
Pf1N1B4_847 -2.8 -7.7 2-isopropylmalate synthase (EC 2.3.3.13) compare
Pf1N1B4_2384 -2.8 -11.1 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) compare
Pf1N1B4_2282 -2.8 -9.7 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf1N1B4_1434 -2.7 -6.5 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf1N1B4_2893 -2.7 -6.5 Carbonic anhydrase (EC 4.2.1.1) compare
Pf1N1B4_767 -2.6 -6.6 Glycerol kinase (EC 2.7.1.30) compare
Pf1N1B4_2490 -2.6 -7.3 Biotin synthesis protein BioC compare
Pf1N1B4_2011 -2.6 -8.1 Pyruvate carboxyl transferase subunit B (EC 6.4.1.1) compare
Pf1N1B4_4166 -2.6 -2.8 Transcriptional regulator, LysR family compare
Pf1N1B4_12 -2.6 -5.6 3-isopropylmalate dehydrogenase (EC 1.1.1.85) compare
Pf1N1B4_1474 -2.6 -6.3 Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42) compare
Pf1N1B4_1460 -2.6 -10.3 Lipid A core - O-antigen ligase and related enzymes compare
Pf1N1B4_1067 -2.5 -3.6 ATP phosphoribosyltransferase (EC 2.4.2.17) (from data) compare
Pf1N1B4_4729 -2.5 -2.7 Hemolysin-type calcium-binding region compare
Pf1N1B4_762 -2.5 -5.9 Ornithine carbamoyltransferase (EC 2.1.3.3) compare
Pf1N1B4_2912 -2.5 -3.1 GTP-binding and nucleic acid-binding protein YchF compare
Pf1N1B4_621 -2.5 -9.6 Glycine dehydrogenase [decarboxylating] (glycine cleavage system P protein) (EC 1.4.4.2) compare
Pf1N1B4_4673 -2.5 -3.7 4Fe-4S ferredoxin, iron-sulfur binding compare
Pf1N1B4_2850 -2.5 -9.3 Glucose-6-phosphate isomerase (EC 5.3.1.9) compare
Pf1N1B4_2949 -2.4 -1.8 FIG00956396: hypothetical protein compare
Pf1N1B4_2283 -2.4 -1.2 Adenosine (5')-pentaphospho-(5'')-adenosine pyrophosphohydrolase (EC 3.6.1.-) compare
Pf1N1B4_3833 -2.4 -9.0 Major porin and structural outer membrane porin OprF compare
Pf1N1B4_2377 -2.3 -7.8 Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC 2.6.1.62) compare
Pf1N1B4_2331 -2.3 -4.9 Thiazole biosynthesis protein ThiG compare
Pf1N1B4_4957 -2.3 -1.6 Transcriptional regulator, TetR family compare
Pf1N1B4_2621 -2.3 -2.0 PspA/IM30 family protein compare
Pf1N1B4_3991 -2.3 -2.6 Predicted transcriptional regulator LiuR of leucine degradation pathway, MerR family compare
Pf1N1B4_218 -2.3 -5.0 Periplasmic binding protein compare
Pf1N1B4_1463 -2.2 -9.6 hypothetical protein compare
Pf1N1B4_598 -2.2 -3.7 Integral membrane sensor signal transduction histidine kinase (EC 2.7.13.3), glucose catabolism cluster compare
Pf1N1B4_1 -2.2 -2.6 Colicin V production protein compare
Pf1N1B4_5611 -2.2 -2.3 L-carnitine dehydratase/bile acid-inducible protein F (EC 2.8.3.16) compare
Pf1N1B4_2337 -2.2 -4.5 Homoserine O-acetyltransferase (EC 2.3.1.31) compare
Pf1N1B4_1750 -2.2 -1.5 Death on curing protein, Doc toxin compare
Pf1N1B4_3906 -2.1 -5.1 DNA topoisomerase I (EC 5.99.1.2) compare
Pf1N1B4_3519 -2.1 -2.9 Lipase (EC 3.1.1.3) compare
Pf1N1B4_1447 -2.1 -10.0 Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17) compare
Pf1N1B4_4550 -2.1 -2.3 beta-ketoacyl synthase, putative compare
Pf1N1B4_835 -2.1 -3.5 Trehalose-6-phosphate hydrolase (EC 3.2.1.93) compare
Pf1N1B4_5690 -2.1 -3.3 Putative TEGT family carrier/transport protein compare
Pf1N1B4_1461 -2.1 -4.4 Protein fixF compare
Pf1N1B4_2112 -2.1 -12.2 Bona fide RidA/YjgF/TdcF/RutC subgroup compare
Pf1N1B4_3518 -2.1 -3.3 Glycerol-3-phosphate dehydrogenase [NAD(P)+] (EC 1.1.1.94) compare
Pf1N1B4_275 -2.0 -2.1 Organic hydroperoxide resistance transcriptional regulator compare
Pf1N1B4_5376 -2.0 -2.1 hypothetical protein compare
Pf1N1B4_3700 -2.0 -3.6 Putative permease often clustered with de novo purine synthesis compare
Pf1N1B4_1611 -2.0 -4.0 Nitrogen regulation protein NR(I) compare
Pf1N1B4_2322 -2.0 -2.3 FIG015547: peptidase, M16 family compare
Pf1N1B4_124 -2.0 -1.8 Mechanosensitive ion channel compare
Pf1N1B4_4731 -2.0 -4.3 Sigma-54 dependent transcriptional regulator compare
Pf1N1B4_2004 -2.0 -2.6 putative membrane protein compare
Pf1N1B4_4669 -2.0 -1.9 Heavy metal RND efflux outer membrane protein, CzcC family compare
Pf1N1B4_3742 -2.0 -2.2 Oxidoreductase, zinc-binding compare
Pf1N1B4_3930 -2.0 -3.5 Shikimate 5-dehydrogenase I gamma (EC 1.1.1.25) compare
Pf1N1B4_5722 -2.0 -1.7 Periplasmic protein p19 involved in high-affinity Fe2+ transport compare
Pf1N1B4_1147 -2.0 -6.5 Fructose repressor FruR, LacI family compare
Pf1N1B4_4592 -2.0 -1.8 Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1) compare
Pf1N1B4_2488 -2.0 -5.5 8-amino-7-oxononanoate synthase (EC 2.3.1.47) compare
Pf1N1B4_2734 -1.9 -2.1 Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system compare
Pf1N1B4_3081 -1.9 -15.2 [Protein-PII] uridylyltransferase (EC 2.7.7.59) compare
Pf1N1B4_27 -1.9 -2.8 Biosynthetic Aromatic amino acid aminotransferase alpha (EC 2.6.1.57) @ Aromatic-amino-acid aminotransferase (EC 2.6.1.57) compare
Pf1N1B4_1906 -1.9 -4.1 Shikimate 5-dehydrogenase I alpha (EC 1.1.1.25) compare
Pf1N1B4_76 -1.9 -3.1 ATP binding protein compare
Pf1N1B4_799 -1.9 -1.9 hypothetical protein compare
Pf1N1B4_814 -1.8 -2.7 Transcriptional regulator, PadR family compare
Pf1N1B4_5817 -1.8 -2.2 Decarboxylase family protein compare
Pf1N1B4_2493 -1.8 -2.5 Acyl-CoA dehydrogenase (EC 1.3.8.7) compare
Pf1N1B4_1625 -1.8 -3.5 FIG00956267: hypothetical protein compare
Pf1N1B4_583 -1.8 -2.6 FIG00957748: hypothetical protein compare
Pf1N1B4_3697 -1.8 -5.4 Phosphoribosylglycinamide formyltransferase (EC 2.1.2.2) compare
Pf1N1B4_335 -1.8 -9.1 Glycosyltransferase compare
Pf1N1B4_2854 -1.8 -3.7 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11) compare
Pf1N1B4_1993 -1.8 -1.6 C4-type zinc finger protein, DksA/TraR family compare
Pf1N1B4_3484 -1.8 -4.8 Sensor protein PhoQ (EC 2.7.13.3) compare
Pf1N1B4_4960 -1.7 -2.5 Protein containing transglutaminase-like domain, putative cysteine protease compare
Pf1N1B4_2823 -1.7 -2.0 COG1496: Uncharacterized conserved protein compare
Pf1N1B4_5976 -1.7 -2.3 1-pyrroline-4-hydroxy-2-carboxylate deaminase (EC 3.5.4.22) compare
Pf1N1B4_2859 -1.7 -9.4 Two-component sensor CbrA: intrcellular carbon:nitrogen balance compare
Pf1N1B4_4518 -1.7 -1.9 Possible pyrimidine permease in reductive pathway compare
Pf1N1B4_2169 -1.7 -2.3 FIG00953808: hypothetical protein compare
Pf1N1B4_3111 -1.7 -2.0 tRNA pseudouridine 13 synthase (EC 4.2.1.-) compare
Pf1N1B4_678 -1.7 -6.7 Pyruvate kinase (EC 2.7.1.40) compare
Pf1N1B4_611 -1.7 -2.7 CoA transferase, CAIB/BAIF family compare
Pf1N1B4_1566 -1.7 -5.3 phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31) (from data) compare
Pf1N1B4_2487 -1.7 -5.3 Biotin synthase (EC 2.8.1.6) compare
Pf1N1B4_654 -1.7 -5.0 Mutator mutT protein (7,8-dihydro-8-oxoguanine-triphosphatase) (EC 3.6.1.-) / Thiamin-phosphate pyrophosphorylase-like protein compare
Pf1N1B4_5604 -1.7 -2.3 Permeases of the major facilitator superfamily compare
Pf1N1B4_430 -1.6 -1.7 hypothetical protein compare
Pf1N1B4_2739 -1.6 -1.5 acetyltransferase, GNAT family compare
Pf1N1B4_1234 -1.6 -3.9 Exodeoxyribonuclease V alpha chain (EC 3.1.11.5) compare
Pf1N1B4_3091 -1.6 -3.6 Membrane-associated zinc metalloprotease compare
Pf1N1B4_4887 -1.6 -2.6 Pirin compare
Pf1N1B4_340 -1.6 -5.2 Alpha-L-Rha alpha-1,3-L-rhamnosyltransferase (EC 2.4.1.-) compare
Pf1N1B4_3670 -1.6 -3.9 probable glucosyl transferase compare
Pf1N1B4_2005 -1.5 -1.6 ATP-dependent DNA helicase UvrD/PcrA compare
Pf1N1B4_3528 -1.5 -3.5 Putative membrane protein compare
Pf1N1B4_1128 -1.5 -2.3 hypothetical protein compare
Pf1N1B4_257 -1.5 -5.5 sensor histidine kinase/response regulator compare
Pf1N1B4_2143 -1.5 -6.1 Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system compare
Pf1N1B4_2886 -1.5 -8.6 Paraquat-inducible protein B compare
Pf1N1B4_3073 -1.5 -4.4 probable glucosyl transferase compare
Pf1N1B4_3636 -1.5 -1.4 FIG00953562: hypothetical protein compare
Pf1N1B4_2925 -1.5 -1.8 hypothetical protein compare
Pf1N1B4_4568 -1.5 -1.4 FIG00793915: hypothetical protein compare
Pf1N1B4_2547 -1.5 -2.3 Anthranilate synthase, amidotransferase component (EC 4.1.3.27) @ Para-aminobenzoate synthase, amidotransferase component (EC 2.6.1.85) compare
Pf1N1B4_1780 -1.5 -1.7 FIG00961586: hypothetical protein compare
Pf1N1B4_2706 -1.5 -2.8 FIG00954871: hypothetical protein compare
Pf1N1B4_349 -1.5 -4.0 Biosynthetic Aromatic amino acid aminotransferase beta (EC 2.6.1.57) compare
Pf1N1B4_1144 -1.4 -6.2 PTS system, fructose-specific IIB component (EC 2.7.1.69) / PTS system, fructose-specific IIC component (EC 2.7.1.69) compare
Pf1N1B4_2924 -1.4 -3.3 FIG00955589: hypothetical protein compare
Pf1N1B4_384 -1.4 -2.0 proteinase inhibitor compare
Pf1N1B4_3520 -1.4 -3.2 Phosphohistidine phosphatase SixA compare
Pf1N1B4_3195 -1.4 -4.7 hydrolase, putative compare
Pf1N1B4_4334 -1.4 -3.1 Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain compare
Pf1N1B4_5619 -1.4 -3.9 BarA-associated response regulator UvrY (= GacA = SirA) compare
Pf1N1B4_2105 -1.4 -2.1 ATP-dependent DNA helicase RecG (EC 3.6.1.-) compare
Pf1N1B4_959 -1.4 -9.3 Stringent starvation protein A compare
Pf1N1B4_2701 -1.4 -11.3 Membrane-bound lytic murein transglycosylase B precursor (EC 3.2.1.-) compare
Pf1N1B4_4566 -1.4 -2.3 Universal stress protein family 7 compare
Pf1N1B4_4826 -1.4 -2.2 glutamine synthetase family protein compare
Pf1N1B4_2958 -1.4 -2.1 Medium-chain-fatty-acid--CoA ligase (EC 6.2.1.-) compare
Pf1N1B4_2042 -1.4 -4.1 Phosphate regulon metal ion transporter containing CBS domains compare
Pf1N1B4_2173 -1.4 -2.2 Diaminopimelate epimerase (EC 5.1.1.7) compare
Pf1N1B4_157 -1.4 -2.1 ThiJ/PfpI family protein compare
Pf1N1B4_890 -1.4 -2.7 DNA recombination and repair protein RecO compare
Pf1N1B4_3517 -1.3 -1.8 hypothetical protein compare
Pf1N1B4_2713 -1.3 -1.6 FIG00955339: hypothetical protein compare
Pf1N1B4_5451 -1.3 -2.8 hypothetical protein compare
Pf1N1B4_3779 -1.3 -1.9 Hydrolase, alpha/beta fold family functionally coupled to Phosphoribulokinase compare
Pf1N1B4_636 -1.3 -4.3 4-hydroxybenzoyl-CoA thioesterase family active site compare
Pf1N1B4_3936 -1.3 -1.9 Transcriptional regulator, LysR family compare
Pf1N1B4_2147 -1.3 -3.0 Putrescine utilization regulator compare
Pf1N1B4_3390 -1.3 -1.7 RNA polymerase sigma factor RpoE compare
Pf1N1B4_1733 -1.3 -5.9 5-aminovalerate aminotransferase (EC 2.6.1.48) / Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19) compare


Specific Phenotypes

For 25 genes in this experiment