Experiment set5IT026 for Bacteroides thetaiotaomicron VPI-5482

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Defined media with Nickel (II) chloride hexahydrate 0.1 mM

Group: stress
Media: Varel_Bryant_medium_Glucose + Nickel (II) chloride hexahydrate (0.1 mM)
Culturing: Btheta_ML6a, 24-well transparent microplate, Anaerobic, at 37 (C), shaken=0 rpm
By: Hualan on 12/19/17
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, 20 mM D-Glucose, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)
Growth plate: 1945 A3

Specific Phenotypes

None in this experiment

For Bacteroides thetaiotaomicron VPI-5482 in stress experiments

For stress Nickel (II) chloride hexahydrate across organisms