Experiment set5IT024 for Pseudomonas fluorescens FW300-N1B4

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Minimal media pH7

200 most important genes:

  gene name fitness t score description  
Pf1N1B4_6 -5.2 -5.1 Phosphoribosylanthranilate isomerase (EC 5.3.1.24) compare
Pf1N1B4_5666 -4.6 -4.5 Oxidoreductase probably involved in sulfite reduction compare
Pf1N1B4_15 -4.5 -5.4 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33) compare
Pf1N1B4_5665 -4.5 -7.4 Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2) compare
Pf1N1B4_2880 -4.3 -15.2 Ketol-acid reductoisomerase (EC 1.1.1.86) compare
Pf1N1B4_1533 -4.2 -10.6 Glutamate synthase [NADPH] small chain (EC 1.4.1.13) compare
Pf1N1B4_1627 -4.2 -5.7 Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19) compare
Pf1N1B4_1307 -3.9 -4.6 3-dehydroquinate dehydratase II (EC 4.2.1.10) compare
Pf1N1B4_2486 -3.8 -3.7 hypothetical protein compare
Pf1N1B4_2545 -3.8 -18.4 Anthranilate synthase, aminase component (EC 4.1.3.27) compare
Pf1N1B4_2547 -3.8 -5.1 Anthranilate synthase, amidotransferase component (EC 4.1.3.27) @ Para-aminobenzoate synthase, amidotransferase component (EC 2.6.1.85) compare
Pf1N1B4_1831 -3.8 -14.0 Tryptophan synthase beta chain (EC 4.2.1.20) compare
Pf1N1B4_1534 -3.7 -23.0 Glutamate synthase [NADPH] large chain (EC 1.4.1.13) compare
Pf1N1B4_2879 -3.6 -9.2 Acetolactate synthase small subunit (EC 2.2.1.6) compare
Pf1N1B4_2303 -3.5 -15.4 Dihydroxy-acid dehydratase (EC 4.2.1.9) compare
Pf1N1B4_350 -3.5 -10.4 Chorismate mutase I (EC 5.4.99.5) / Prephenate dehydratase (EC 4.2.1.51) compare
Pf1N1B4_1624 -3.4 -10.4 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data) compare
Pf1N1B4_2384 -3.3 -12.8 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) compare
Pf1N1B4_1408 -3.3 -4.1 ATP phosphoribosyltransferase regulatory subunit (EC 2.4.2.17) compare
Pf1N1B4_2112 -3.3 -17.9 Bona fide RidA/YjgF/TdcF/RutC subgroup compare
Pf1N1B4_2272 -3.3 -6.2 D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95) compare
Pf1N1B4_12 -3.2 -9.3 3-isopropylmalate dehydrogenase (EC 1.1.1.85) compare
Pf1N1B4_58 -3.1 -8.8 Transaldolase (EC 2.2.1.2) compare
Pf1N1B4_2338 -3.1 -10.1 Homoserine O-acetyltransferase (EC 2.3.1.31) compare
Pf1N1B4_1434 -3.1 -8.7 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf1N1B4_2548 -2.9 -7.6 Anthranilate phosphoribosyltransferase (EC 2.4.2.18) compare
Pf1N1B4_1565 -2.9 -5.5 Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19) compare
Pf1N1B4_4065 -2.8 -9.1 Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107) compare
Pf1N1B4_2337 -2.8 -7.1 Homoserine O-acetyltransferase (EC 2.3.1.31) compare
Pf1N1B4_1626 -2.8 -8.3 Imidazole glycerol phosphate synthase amidotransferase subunit (EC 2.4.2.-) compare
Pf1N1B4_2549 -2.8 -9.1 Indole-3-glycerol phosphate synthase (EC 4.1.1.48) compare
Pf1N1B4_2858 -2.8 -12.8 Two-component sensor CbrB: intrcellular carbon:nitrogen balance compare
Pf1N1B4_2011 -2.8 -11.1 Pyruvate carboxyl transferase subunit B (EC 6.4.1.1) compare
Pf1N1B4_1830 -2.7 -8.9 Tryptophan synthase alpha chain (EC 4.2.1.20) compare
Pf1N1B4_762 -2.7 -8.3 Ornithine carbamoyltransferase (EC 2.1.3.3) compare
Pf1N1B4_2009 -2.7 -6.8 LysR family transcriptional regulator PA5437 compare
Pf1N1B4_2282 -2.7 -10.7 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf1N1B4_1067 -2.7 -5.0 ATP phosphoribosyltransferase (EC 2.4.2.17) (from data) compare
Pf1N1B4_3697 -2.7 -8.5 Phosphoribosylglycinamide formyltransferase (EC 2.1.2.2) compare
Pf1N1B4_2878 -2.6 -9.9 Acetolactate synthase large subunit (EC 2.2.1.6) compare
Pf1N1B4_3812 -2.6 -11.6 Phosphoadenylyl-sulfate reductase [thioredoxin] (EC 1.8.4.8) / Adenylyl-sulfate reductase [thioredoxin] (EC 1.8.4.10) compare
Pf1N1B4_742 -2.6 -6.5 Argininosuccinate synthase (EC 6.3.4.5) compare
Pf1N1B4_2488 -2.5 -8.2 8-amino-7-oxononanoate synthase (EC 2.3.1.47) compare
Pf1N1B4_847 -2.4 -7.4 2-isopropylmalate synthase (EC 2.3.3.13) compare
Pf1N1B4_2874 -2.4 -2.8 Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-) compare
Pf1N1B4_1625 -2.4 -3.8 FIG00956267: hypothetical protein compare
Pf1N1B4_1315 -2.4 -7.2 IMP cyclohydrolase (EC 3.5.4.10) / Phosphoribosylaminoimidazolecarboxamide formyltransferase (EC 2.1.2.3) compare
Pf1N1B4_27 -2.4 -7.1 Biosynthetic Aromatic amino acid aminotransferase alpha (EC 2.6.1.57) @ Aromatic-amino-acid aminotransferase (EC 2.6.1.57) compare
Pf1N1B4_3153 -2.2 -3.3 hypothetical protein compare
Pf1N1B4_1535 -2.2 -4.8 hypothetical protein compare
Pf1N1B4_2491 -2.2 -4.5 Dethiobiotin synthetase (EC 6.3.3.3) compare
Pf1N1B4_2010 -2.2 -6.9 Pyruvate carboxyl transferase subunit A (EC 6.4.1.1) compare
Pf1N1B4_1906 -2.1 -4.8 Shikimate 5-dehydrogenase I alpha (EC 1.1.1.25) compare
Pf1N1B4_2490 -2.1 -6.1 Biotin synthesis protein BioC compare
Pf1N1B4_2489 -2.1 -5.5 Biotin synthesis protein BioH compare
Pf1N1B4_2850 -2.1 -9.6 Glucose-6-phosphate isomerase (EC 5.3.1.9) compare
Pf1N1B4_2280 -2.0 -8.8 Threonine dehydratase biosynthetic (EC 4.3.1.19) compare
Pf1N1B4_1 -2.0 -3.2 Colicin V production protein compare
Pf1N1B4_2377 -2.0 -7.3 Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC 2.6.1.62) compare
Pf1N1B4_603 -2.0 -4.9 Methylglyoxal synthase (EC 4.2.3.3) compare
Pf1N1B4_3506 -1.9 -6.7 Cysteine synthase B (EC 2.5.1.47) compare
Pf1N1B4_2621 -1.9 -2.0 PspA/IM30 family protein compare
Pf1N1B4_2487 -1.8 -7.1 Biotin synthase (EC 2.8.1.6) compare
Pf1N1B4_1329 -1.8 -7.2 Cobalamin biosynthesis protein CobG compare
Pf1N1B4_3833 -1.8 -9.3 Major porin and structural outer membrane porin OprF compare
Pf1N1B4_3168 -1.7 -3.3 AmpG permease compare
Pf1N1B4_5991 -1.7 -3.2 Transcriptional regulator, AraC family compare
Pf1N1B4_1610 -1.7 -4.5 Nitrogen regulation protein NtrB (EC 2.7.13.3) compare
Pf1N1B4_6041 -1.6 -2.9 Carbon storage regulator compare
Pf1N1B4_3081 -1.6 -13.1 [Protein-PII] uridylyltransferase (EC 2.7.7.59) compare
Pf1N1B4_49 -1.6 -4.2 hypothetical protein compare
Pf1N1B4_3907 -1.6 -3.4 FIG00953287: hypothetical protein compare
Pf1N1B4_1993 -1.6 -2.2 C4-type zinc finger protein, DksA/TraR family compare
Pf1N1B4_1153 -1.5 -4.0 Quinolinate phosphoribosyltransferase [decarboxylating] (EC 2.4.2.19) compare
Pf1N1B4_5284 -1.5 -3.2 hypothetical protein compare
Pf1N1B4_767 -1.5 -4.7 Glycerol kinase (EC 2.7.1.30) compare
Pf1N1B4_4957 -1.5 -2.8 Transcriptional regulator, TetR family compare
Pf1N1B4_2037 -1.4 -3.7 Phosphate transport ATP-binding protein PstB (TC 3.A.1.7.1) compare
Pf1N1B4_1566 -1.4 -4.9 phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31) (from data) compare
Pf1N1B4_3518 -1.4 -2.6 Glycerol-3-phosphate dehydrogenase [NAD(P)+] (EC 1.1.1.94) compare
Pf1N1B4_3779 -1.3 -2.2 Hydrolase, alpha/beta fold family functionally coupled to Phosphoribulokinase compare
Pf1N1B4_2859 -1.3 -8.7 Two-component sensor CbrA: intrcellular carbon:nitrogen balance compare
Pf1N1B4_3692 -1.3 -3.7 Chaperone protein HtpG compare
Pf1N1B4_1057 -1.3 -2.2 Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4) / Adenylylsulfate kinase (EC 2.7.1.25) compare
Pf1N1B4_2224 -1.3 -2.6 FIG00953390: hypothetical protein compare
Pf1N1B4_652 -1.3 -7.0 Sensory box histidine kinase compare
Pf1N1B4_578 -1.2 -1.6 enoyl-CoA hydratase, R-specific compare
Pf1N1B4_626 -1.2 -5.8 Quinolinate synthetase (EC 2.5.1.72) compare
Pf1N1B4_1328 -1.2 -2.3 Cobalt-precorrin-8x methylmutase (EC 5.4.1.2) compare
Pf1N1B4_718 -1.2 -5.1 outer membrane component of uptake system, probably for ferrous iron (from data) compare
Pf1N1B4_716 -1.2 -2.6 Probable thiol oxidoreductase with 2 cytochrome c heme-binding sites compare
Pf1N1B4_3143 -1.2 -3.9 RecA protein compare
Pf1N1B4_3451 -1.2 -3.5 Aspartokinase (EC 2.7.2.4) compare
Pf1N1B4_3709 -1.2 -3.9 Adenosylcobinamide-phosphate synthase (EC 6.3.1.10) compare
Pf1N1B4_2912 -1.2 -2.0 GTP-binding and nucleic acid-binding protein YchF compare
Pf1N1B4_715 -1.2 -4.5 Iron-regulated protein A precursor compare
Pf1N1B4_267 -1.1 -1.8 Enoyl-CoA hydratase (EC 4.2.1.17) compare
Pf1N1B4_5724 -1.1 -2.0 High-affinity iron permease compare
Pf1N1B4_1317 -1.1 -1.9 Sensor histidine kinase/response regulator compare
Pf1N1B4_5554 -1.1 -2.6 hypothetical protein compare
Pf1N1B4_1837 -1.1 -1.7 Osmotically inducible protein C compare
Pf1N1B4_3443 -1.1 -1.6 Arginine N-succinyltransferase (EC 2.3.1.109) compare
Pf1N1B4_602 -1.1 -2.4 NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12) compare
Pf1N1B4_717 -1.0 -5.3 Iron-regulated protein A precursor compare
Pf1N1B4_3468 -1.0 -2.0 Negative regulator of flagellin synthesis FlgM compare
Pf1N1B4_3152 -1.0 -3.4 LysR family transcriptional regulator PA3398 compare
Pf1N1B4_1637 -1.0 -1.9 FIG00954739: hypothetical protein compare
Pf1N1B4_5653 -1.0 -4.2 CobN component of cobalt chelatase involved in B12 biosynthesis compare
Pf1N1B4_3715 -1.0 -3.2 Cobalamin synthase compare
Pf1N1B4_1611 -1.0 -3.2 Nitrogen regulation protein NR(I) compare
Pf1N1B4_1475 -1.0 -4.1 FIG00962376: hypothetical protein compare
Pf1N1B4_4028 -1.0 -3.0 NADH-ubiquinone oxidoreductase chain N (EC 1.6.5.3) compare
Pf1N1B4_618 -1.0 -3.2 Acetyl-CoA carboxylase alpha subunit compare
Pf1N1B4_5492 -1.0 -3.0 hypothetical protein compare
Pf1N1B4_3078 -1.0 -2.2 FIG138056: a glutathione-dependent thiol reductase compare
Pf1N1B4_4474 -0.9 -2.6 transcription regulator protein compare
Pf1N1B4_5310 -0.9 -2.6 hypothetical protein compare
Pf1N1B4_2071 -0.9 -1.7 Protein phosphatase ImpM compare
Pf1N1B4_4035 -0.9 -1.7 NADH-ubiquinone oxidoreductase chain G (EC 1.6.5.3) compare
Pf1N1B4_2048 -0.9 -1.1 hypothetical protein compare
Pf1N1B4_974 -0.9 -3.0 Periplasmic thiol:disulfide oxidoreductase DsbB, required for DsbA reoxidation compare
Pf1N1B4_76 -0.9 -1.5 ATP binding protein compare
Pf1N1B4_1340 -0.9 -2.1 ABC transporter (iron.B12.siderophore.hemin) , periplasmic substrate-binding component compare
Pf1N1B4_4776 -0.9 -2.5 Nicotinamidase (EC 3.5.1.19) compare
Pf1N1B4_1971 -0.9 -2.6 SAM-dependent methyltransferases compare
Pf1N1B4_3711 -0.8 -4.8 Cobyric acid synthase (EC 6.3.5.10) compare
Pf1N1B4_4368 -0.8 -3.1 Guanosine-3',5'-bis(Diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2) compare
Pf1N1B4_5109 -0.8 -2.6 Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases compare
Pf1N1B4_2453 -0.8 -1.4 hypothetical protein compare
Pf1N1B4_3486 -0.8 -2.6 Transcriptional regulator, AraC family compare
Pf1N1B4_139 -0.8 -1.5 Pyoverdin biosynthesis protein PvdN, putative aminotransferase, class V compare
Pf1N1B4_2283 -0.8 -1.7 Adenosine (5')-pentaphospho-(5'')-adenosine pyrophosphohydrolase (EC 3.6.1.-) compare
Pf1N1B4_2209 -0.8 -5.3 Exopolyphosphatase (EC 3.6.1.11) compare
Pf1N1B4_3768 -0.8 -2.0 Homoserine/homoserine lactone efflux protein compare
Pf1N1B4_338 -0.8 -1.5 Alpha-L-Rha alpha-1,3-L-rhamnosyltransferase (EC 2.4.1.-) compare
Pf1N1B4_1346 -0.8 -2.3 Urea ABC transporter, ATPase protein UrtD compare
Pf1N1B4_458 -0.8 -3.4 Similar to phosphoglycolate phosphatase, clustered with ribosomal large subunit pseudouridine synthase C compare
Pf1N1B4_2885 -0.8 -3.8 Paraquat-inducible protein A compare
Pf1N1B4_5659 -0.8 -6.1 NfuA Fe-S protein maturation compare
Pf1N1B4_722 -0.8 -1.4 SAM-dependent methyltransferases compare
Pf1N1B4_1331 -0.8 -2.5 Cobalt-precorrin-6y C5-methyltransferase (EC 2.1.1.-) / Cobalt-precorrin-6y C15-methyltransferase [decarboxylating] (EC 2.1.1.-) compare
Pf1N1B4_1668 -0.8 -3.7 3'(2'),5'-bisphosphate nucleotidase (EC 3.1.3.7) compare
Pf1N1B4_4201 -0.8 -2.9 Transcriptional regulator, LysR family compare
Pf1N1B4_4824 -0.8 -1.8 Gamma-glutamyl-putrescine oxidase (EC1.4.3.-) compare
Pf1N1B4_4140 -0.8 -2.3 hypothetical protein compare
Pf1N1B4_364 -0.8 -2.5 Rhodanese domain protein UPF0176 compare
Pf1N1B4_2075 -0.8 -2.3 ABC-type antimicrobial peptide transport system, permease component compare
Pf1N1B4_4950 -0.8 -2.6 formate dehydrogenase formation protein FdhE compare
Pf1N1B4_2225 -0.8 -5.5 5-formyltetrahydrofolate cyclo-ligase (EC 6.3.3.2) compare
Pf1N1B4_2568 -0.8 -1.8 Translation elongation factor Tu compare
Pf1N1B4_339 -0.8 -1.7 UDP-glucose 4-epimerase (EC 5.1.3.2) compare
Pf1N1B4_5823 -0.8 -1.5 Na(+) H(+) antiporter subunit C compare
Pf1N1B4_6035 -0.8 -2.5 D-ribose ABC transporter, substrate-binding component RbsB (from data) compare
Pf1N1B4_5512 -0.8 -1.6 hypothetical protein compare
Pf1N1B4_693 -0.8 -2.5 ABC spermidine/putrescine transporter, inner membrane subunit compare
Pf1N1B4_4759 -0.8 -1.3 Proteinase inhibitor I11, ecotin precursor compare
Pf1N1B4_4040 -0.8 -1.8 NADH-ubiquinone oxidoreductase chain B (EC 1.6.5.3) compare
Pf1N1B4_4472 -0.8 -2.3 Transcriptional regulator, ArsR family compare
Pf1N1B4_4544 -0.8 -1.9 Uncharacterized protein ImpA compare
Pf1N1B4_3546 -0.7 -1.6 P pilus assembly/Cpx signaling pathway, periplasmic inhibitor/zinc-resistance associated protein compare
Pf1N1B4_1671 -0.7 -1.7 FIG00954962: hypothetical protein compare
Pf1N1B4_34 -0.7 -1.8 Xanthine permease compare
Pf1N1B4_5349 -0.7 -2.5 hypothetical protein compare
Pf1N1B4_3938 -0.7 -2.3 Integral membrane protein TerC compare
Pf1N1B4_3372 -0.7 -2.1 FIG00962464: hypothetical protein compare
Pf1N1B4_516 -0.7 -2.0 Sensor histidine kinase compare
Pf1N1B4_3098 -0.7 -2.0 Ribonuclease HII (EC 3.1.26.4) compare
Pf1N1B4_2877 -0.7 -3.8 Glycosyltransferase compare
Pf1N1B4_4828 -0.7 -1.4 hypothetical protein compare
Pf1N1B4_4460 -0.7 -1.9 Cytochrome b561 compare
Pf1N1B4_4857 -0.7 -3.2 Chemotaxis protein methyltransferase CheR (EC 2.1.1.80) compare
Pf1N1B4_3819 -0.7 -3.2 Methylisocitrate lyase (EC 4.1.3.30) compare
Pf1N1B4_644 -0.7 -1.7 Cold shock protein CspC compare
Pf1N1B4_4041 -0.7 -2.0 NADH ubiquinone oxidoreductase chain A (EC 1.6.5.3) compare
Pf1N1B4_616 -0.7 -2.2 Ornithine carbamoyltransferase (EC 2.1.3.3) compare
Pf1N1B4_733 -0.7 -1.1 Methionyl-tRNA synthetase (EC 6.1.1.10) compare
Pf1N1B4_2171 -0.7 -2.4 Tyrosine recombinase XerC compare
Pf1N1B4_6038 -0.7 -2.4 FIG00953874: hypothetical protein compare
Pf1N1B4_4823 -0.7 -2.6 Paraquat-inducible protein A compare
Pf1N1B4_3396 -0.7 -1.5 Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1) compare
Pf1N1B4_828 -0.7 -3.4 Multicopper oxidase compare
Pf1N1B4_1485 -0.7 -2.1 FIG00963848: hypothetical protein compare
Pf1N1B4_5275 -0.7 -1.8 hypothetical protein compare
Pf1N1B4_3707 -0.7 -3.6 Cobyrinic acid A,C-diamide synthase compare
Pf1N1B4_4755 -0.7 -1.8 ribosomal protein S6 glutaminyl transferase related protein compare
Pf1N1B4_4830 -0.7 -1.9 Membrane fusion component of tripartite multidrug resistance system compare
Pf1N1B4_4715 -0.7 -1.7 Transcriptional regulator compare
Pf1N1B4_5330 -0.7 -0.7 hypothetical protein compare
Pf1N1B4_1996 -0.7 -2.7 NADH:ubiquinone oxidoreductase 49 kD subunit 7 compare
Pf1N1B4_4470 -0.7 -2.2 Cobalt-zinc-cadmium resistance protein CzcD compare
Pf1N1B4_2924 -0.7 -1.5 FIG00955589: hypothetical protein compare
Pf1N1B4_5242 -0.7 -1.5 hypothetical protein compare
Pf1N1B4_3364 -0.7 -1.7 Cyn operon transcriptional activator compare
Pf1N1B4_1353 -0.7 -2.1 Urease alpha subunit (EC 3.5.1.5) compare
Pf1N1B4_2210 -0.7 -1.4 Glutamate transport ATP-binding protein compare
Pf1N1B4_2959 -0.7 -2.8 FIG067310: hypothetical protein compare
Pf1N1B4_5811 -0.7 -2.7 FIG00958502: hypothetical protein compare
Pf1N1B4_1544 -0.6 -4.1 Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-) compare
Pf1N1B4_600 -0.6 -2.3 Glucokinase (EC 2.7.1.2) compare
Pf1N1B4_1827 -0.6 -2.6 FIG00953482: hypothetical protein compare


Specific Phenotypes

None in this experiment

For Pseudomonas fluorescens FW300-N1B4 in pH experiments