Experiment set5IT022 for Dechlorosoma suillum PS

Compare to:

ALP with Sodium nitrate 75 mM

Group: stress
Media: ALP_richB + Sodium nitrate (75 mM)
Culturing: PS_ML1, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Mark on 4/20/2015
Media components: 0.97 g/L Sodium phosphate dibasic heptahydrate, 0.1 g/L Potassium Chloride, 0.25 g/L Ammonium chloride, 40 mM Sodium D,L-Lactate, 1.1 g/L Sodium pyruvate, 0.8203 g/L Sodium acetate, 2 g/L Yeast Extract, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Growth plate: 1213 B3,B4

Specific Phenotypes

For 2 genes in this experiment

For stress Sodium nitrate in Dechlorosoma suillum PS

For stress Sodium nitrate across organisms

SEED Subsystems

Subsystem #Specific
Peptidoglycan Biosynthesis 2

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
peptidoglycan maturation (meso-diaminopimelate containing) 12 3 2
peptidoglycan biosynthesis IV (Enterococcus faecium) 17 12 1
peptidoglycan biosynthesis II (staphylococci) 17 12 1