Experiment set5IT021 for Bacteroides thetaiotaomicron VPI-5482
Cytidine nitrogen source
Group: nitrogen sourceMedia: Varel_Bryant_medium_noNitrogen + Cytidine (20 mM)
Culturing: Btheta_ML6a, 24-well transparent microplate, Anaerobic, at 37 (C), shaken=0 rpm
By: Hualan on 12/19/17
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, 20 mM D-Glucose, Mineral 3B solution minus Nitrogen (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 1.75 mM Sodium sulfate)
Growth plate: 1944 C3
Specific Phenotypes
For 2 genes in this experiment
For nitrogen source Cytidine in Bacteroides thetaiotaomicron VPI-5482
For nitrogen source Cytidine across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| De Novo Pyrimidine Synthesis | 1 |
| LMPTP YwlE cluster | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| pyrimidine nucleobases salvage I | 1 | 1 | 1 |
| pyrimidine nucleobases salvage II | 2 | 1 | 1 |
| superpathway of pyrimidine nucleobases salvage | 4 | 4 | 1 |
| superpathway of pyrimidine ribonucleosides salvage | 10 | 9 | 1 |