Experiment set5IT020 for Bacteroides thetaiotaomicron VPI-5482

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Thymidine nitrogen source

Group: nitrogen source
Media: Varel_Bryant_medium_noNitrogen + Thymidine (10 mM)
Culturing: Btheta_ML6a, 24-well transparent microplate, Anaerobic, at 37 (C), shaken=0 rpm
By: Hualan on 12/19/17
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, 20 mM D-Glucose, Mineral 3B solution minus Nitrogen (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 1.75 mM Sodium sulfate)
Growth plate: 1944 C1

Specific Phenotypes

For 3 genes in this experiment

For nitrogen source Thymidine in Bacteroides thetaiotaomicron VPI-5482

For nitrogen source Thymidine across organisms

SEED Subsystems

Subsystem #Specific
Deoxyribose and Deoxynucleoside Catabolism 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
acetate and ATP formation from acetyl-CoA III 1 1 1
acetate conversion to acetyl-CoA 1 1 1
superpathway of acetate utilization and formation 3 3 1
L-isoleucine biosynthesis V 3 2 1
ethanol degradation II 3 2 1
2-deoxy-D-ribose degradation I 3 2 1
ethanol degradation IV 3 2 1
ethanol degradation III 3 1 1
2-deoxy-α-D-ribose 1-phosphate degradation 3 1 1
chitin deacetylation 4 1 1
superpathway of pyrimidine deoxyribonucleosides degradation 6 3 1
superpathway of bitter acids biosynthesis 18 3 3
colupulone and cohumulone biosynthesis 6 1 1
adlupulone and adhumulone biosynthesis 6 1 1
lupulone and humulone biosynthesis 6 1 1
superpathway of purine deoxyribonucleosides degradation 7 4 1
reductive glycine pathway of autotrophic CO2 fixation 9 6 1
cis-geranyl-CoA degradation 9 1 1