Experiment set5IT019 for Pseudomonas fluorescens FW300-N2E2

Compare to:

LB with sodium carbonate monohydrate 0.0125 M

200 most detrimental genes:

  gene name fitness t score description  
Pf6N2E2_4459 +1.1 3.0 Transcriptional regulator, GntR family compare
Pf6N2E2_3589 +1.1 3.0 Lysine decarboxylase family compare
Pf6N2E2_672 +1.0 2.7 ABC-type polar amino acid transport system, ATPase component compare
Pf6N2E2_405 +0.9 1.9 hypothetical protein compare
Pf6N2E2_4052 +0.9 1.6 FIG00955481: hypothetical protein compare
Pf6N2E2_5646 +0.9 1.8 PROBABLE MEMBRANE PROTEIN compare
Pf6N2E2_2605 +0.9 3.9 3-oxoacyl-[acyl-carrier-protein] synthase, KASIII (EC 2.3.1.180) compare
Pf6N2E2_1065 +0.9 2.7 Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system compare
Pf6N2E2_1412 +0.8 2.9 hypothetical protein compare
Pf6N2E2_5677 +0.8 1.5 Carbon storage regulator compare
Pf6N2E2_2324 +0.8 3.0 Adenine phosphoribosyltransferase (EC 2.4.2.7) compare
Pf6N2E2_987 +0.8 1.8 hypothetical protein compare
Pf6N2E2_3568 +0.8 2.7 Unsaturated fatty acid biosythesis repressor FabR, TetR family compare
Pf6N2E2_2022 +0.7 4.0 BarA-associated response regulator UvrY (= GacA = SirA) compare
Pf6N2E2_500 +0.7 2.2 Beta-ureidopropionase (EC 3.5.1.6) compare
Pf6N2E2_695 +0.7 2.6 Nitrite reductase associated c-type cytochorome NirN compare
Pf6N2E2_5943 +0.7 1.5 hypothetical protein compare
Pf6N2E2_5862 +0.7 2.0 Lipoprotein, putative compare
Pf6N2E2_414 +0.7 1.8 Chemotaxis protein CheD compare
Pf6N2E2_5340 +0.7 2.6 Stringent starvation protein A compare
Pf6N2E2_4415 +0.7 1.9 Putative FMN hydrolase (EC 3.1.3.-); 5-Amino-6-(5'-phosphoribitylamino)uracil phosphatase compare
Pf6N2E2_4540 +0.7 1.6 hypothetical protein compare
Pf6N2E2_5307 +0.7 3.1 FIG006045: Sigma factor, ECF subfamily compare
Pf6N2E2_2904 +0.7 1.9 Hypothetical protein DUF454 compare
Pf6N2E2_6070 +0.7 1.7 FIG137877: Hypothetical protein in pyoverdin gene cluster compare
Pf6N2E2_2152 +0.7 1.4 hypothetical protein compare
Pf6N2E2_5772 +0.7 1.6 DUF1232 domain-containing protein compare
Pf6N2E2_3305 +0.6 1.6 hypothetical protein compare
Pf6N2E2_3744 +0.6 1.7 General secretion pathway protein F compare
Pf6N2E2_1293 +0.6 1.2 COG2879, Hypothetical small protein yjiX compare
Pf6N2E2_1014 +0.6 1.4 Nitrate/nitrite transporter compare
Pf6N2E2_1701 +0.6 1.6 ABC transporter ATP-binding protein compare
Pf6N2E2_3820 +0.6 2.2 hypothetical protein compare
Pf6N2E2_2363 +0.6 2.0 Type III secretion inner membrane protein (YscT,HrcT,SpaR,EscT,EpaR1,homologous to flagellar export components) compare
Pf6N2E2_2842 +0.6 1.7 hypothetical protein compare
Pf6N2E2_1876 +0.6 1.8 O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) compare
Pf6N2E2_62 +0.6 2.6 Cys regulon transcriptional activator CysB compare
Pf6N2E2_464 +0.6 1.8 Sensory histidine kinase QseC compare
Pf6N2E2_1434 +0.6 1.2 Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3) compare
Pf6N2E2_5613 +0.6 1.8 FIG00953674: hypothetical protein compare
Pf6N2E2_2519 +0.6 0.9 Biosynthetic Aromatic amino acid aminotransferase beta (EC 2.6.1.57) compare
Pf6N2E2_819 +0.6 1.5 Response regulator compare
Pf6N2E2_5758 +0.6 1.2 FIG00957314: hypothetical protein compare
Pf6N2E2_1505 +0.6 2.2 Transporter, MFS superfamily compare
Pf6N2E2_6031 +0.6 1.9 hypothetical protein compare
Pf6N2E2_476 +0.6 1.6 Permease of the drug/metabolite transporter (DMT) superfamily compare
Pf6N2E2_5910 +0.6 1.6 Flp pilus assembly protein RcpC/CpaB compare
Pf6N2E2_41 +0.6 3.3 Two-component system response regulator QseB compare
Pf6N2E2_868 +0.6 1.0 Transcriptional regulator, HxlR family compare
Pf6N2E2_2984 +0.6 1.2 LysR family transcriptional regulator PA3398 compare
Pf6N2E2_2878 +0.6 1.5 required for 4-hydroxybenzoate and octanoate transport, together with MFP and FUSC proteins (Pf6N2E2_2877, Pf6N2E2_2879) (from data) compare
Pf6N2E2_2200 +0.6 1.0 RNA polymerase sigma-54 factor RpoN compare
Pf6N2E2_204 +0.6 0.7 hypothetical protein compare
Pf6N2E2_5720 +0.6 4.6 BarA sensory histidine kinase (= VarS = GacS) compare
Pf6N2E2_3386 +0.6 2.1 hypothetical protein compare
Pf6N2E2_4181 +0.6 0.9 Organic hydroperoxide resistance protein compare
Pf6N2E2_420 +0.6 0.7 Chemotaxis regulator - transmits chemoreceptor signals to flagelllar motor components CheY compare
Pf6N2E2_5252 +0.6 1.3 FIG00956396: hypothetical protein compare
Pf6N2E2_1599 +0.6 2.4 Cytochrome c-type protein NapC compare
Pf6N2E2_533 +0.6 2.2 Na(+) H(+) antiporter subunit G compare
Pf6N2E2_1715 +0.6 1.4 hypothetical protein compare
Pf6N2E2_1688 +0.6 2.0 Permeases of the major facilitator superfamily compare
Pf6N2E2_5468 +0.5 2.0 Nudix hydrolase family protein PA3470 compare
Pf6N2E2_2069 +0.5 1.4 FIG00953703: hypothetical protein compare
Pf6N2E2_1169 +0.5 1.4 TnpR protein compare
Pf6N2E2_733 +0.5 1.4 Peptidyl-prolyl cis-trans isomerase PpiD (EC 5.2.1.8) compare
Pf6N2E2_1691 +0.5 1.4 hypothetical protein compare
Pf6N2E2_2396 +0.5 1.9 Sensor histidine kinase compare
Pf6N2E2_1035 +0.5 2.1 MotA/TolQ/ExbB proton channel family protein compare
Pf6N2E2_5262 +0.5 1.0 FKBP-type peptidyl-prolyl cis-trans isomerase SlyD (EC 5.2.1.8) compare
Pf6N2E2_4332 +0.5 2.4 hypothetical protein compare
Pf6N2E2_4598 +0.5 2.6 COG1872 compare
Pf6N2E2_42 +0.5 2.5 Sensor protein PhoQ (EC 2.7.13.3) compare
Pf6N2E2_4508 +0.5 1.5 Transcriptional regulator, TetR family compare
Pf6N2E2_336 +0.5 1.9 hypothetical protein compare
Pf6N2E2_473 +0.5 1.9 putative cytochrome p450 oxidoreductase compare
Pf6N2E2_5134 +0.5 1.4 FIG060329: MOSC domain protein compare
Pf6N2E2_1648 +0.5 2.4 Maltose/maltodextrin ABC transporter, permease protein MalG compare
Pf6N2E2_2175 +0.5 1.8 Transcriptional regulator, RpiR family compare
Pf6N2E2_5236 +0.5 1.2 hypothetical protein compare
Pf6N2E2_1090 +0.5 1.7 Transcriptional regulator for ferulate or vanillate catabolism compare
Pf6N2E2_413 +0.5 1.8 Chemotaxis response regulator protein-glutamate methylesterase CheB (EC 3.1.1.61) compare
Pf6N2E2_4531 +0.5 1.0 hypothetical protein compare
Pf6N2E2_5621 +0.5 2.0 DNA-binding response regulator compare
Pf6N2E2_2448 +0.5 1.9 Thiopurine S-methyltransferase (EC 2.1.1.67) compare
Pf6N2E2_4635 +0.5 3.5 ATP-dependent RNA helicase RhlE compare
Pf6N2E2_5087 +0.5 1.6 Transcription elongation factor GreB-related protein compare
Pf6N2E2_5510 +0.5 1.4 FIG00955324: hypothetical protein compare
Pf6N2E2_4943 +0.5 1.8 PspA/IM30 family protein compare
Pf6N2E2_460 +0.5 1.2 Glycerate kinase (EC 2.7.1.31) compare
Pf6N2E2_5137 +0.5 1.6 FIG00956650: hypothetical protein compare
Pf6N2E2_4747 +0.5 1.7 Transmembrane sensor compare
Pf6N2E2_1512 +0.5 1.7 Thiamin biosynthesis lipoprotein ApbE compare
Pf6N2E2_1695 +0.5 1.8 Acyl-CoA dehydrogenase; probable dibenzothiophene desulfurization enzyme compare
Pf6N2E2_1804 +0.5 2.2 Immune-responsive protein 1 compare
Pf6N2E2_351 +0.5 1.7 Aldehyde dehydrogenase (EC 1.2.1.3) compare
Pf6N2E2_4095 +0.5 3.7 Carbonic anhydrase (EC 4.2.1.1) compare
Pf6N2E2_1696 +0.5 1.3 Alkanesulfonates-binding protein compare
Pf6N2E2_647 +0.5 1.3 General secretion pathway protein G compare
Pf6N2E2_1759 +0.5 1.5 uncharacterized domain 1 compare
Pf6N2E2_4160 +0.5 0.7 Cytochrome c5 compare
Pf6N2E2_4681 +0.5 2.9 ABC transporter for Carnitine, ATPase component (from data) compare
Pf6N2E2_5643 +0.5 2.2 hypothetical protein compare
Pf6N2E2_3346 +0.5 2.2 Quinolinate phosphoribosyltransferase [decarboxylating] (EC 2.4.2.19) compare
Pf6N2E2_1086 +0.5 2.2 Acyl-homoserine lactone acylase PvdQ (EC 3.5.1.-), quorum-quenching compare
Pf6N2E2_3875 +0.5 1.0 FIG00953324: hypothetical protein compare
Pf6N2E2_5632 +0.5 1.5 Aromatic amino acid transport protein AroP compare
Pf6N2E2_5324 +0.5 2.2 diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s) compare
Pf6N2E2_1534 +0.5 0.6 Probable transmembrane protein compare
Pf6N2E2_2373 +0.5 1.4 Type III secretion thermoregulatory protein (LcrF,VirF,transcription regulation of virulence plasmid) compare
Pf6N2E2_2155 +0.5 1.5 hypothetical protein compare
Pf6N2E2_831 +0.5 1.1 Ketoacyl reductase hetN (EC 1.3.1.-) compare
Pf6N2E2_4061 +0.5 1.5 Rubredoxin compare
Pf6N2E2_969 +0.5 2.3 Putative uncharacterized protein STY4534 (Putative uncharacterized protein) compare
Pf6N2E2_4305 +0.5 0.8 FIG00959590: hypothetical protein compare
Pf6N2E2_6045 +0.4 0.7 hypothetical protein compare
Pf6N2E2_5320 +0.4 2.7 Cytochrome O ubiquinol oxidase subunit IV (EC 1.10.3.-) compare
Pf6N2E2_2208 +0.4 1.5 1-aminocyclopropane-1-carboxylate deaminase (EC 3.5.99.7) compare
Pf6N2E2_550 +0.4 1.8 Uncharacterized protein ImpH/VasB compare
Pf6N2E2_5571 +0.4 1.1 hypothetical protein compare
Pf6N2E2_1500 +0.4 1.7 LysR family transcriptional regulator lrhA compare
Pf6N2E2_2487 +0.4 0.7 FIG00956090: hypothetical protein compare
Pf6N2E2_146 +0.4 0.9 Transcriptional regulator, MarR family compare
Pf6N2E2_1455 +0.4 1.6 Xylose ABC transporter, periplasmic xylose-binding protein XylF compare
Pf6N2E2_1579 +0.4 1.6 hypothetical protein compare
Pf6N2E2_1161 +0.4 1.6 FIG00961921: hypothetical protein compare
Pf6N2E2_1074 +0.4 0.9 FIG00953356: hypothetical protein compare
Pf6N2E2_1223 +0.4 1.5 Amino acid transporters compare
Pf6N2E2_4059 +0.4 0.7 DNA-binding protein HU-alpha compare
Pf6N2E2_1885 +0.4 1.3 Transcriptional regulator, AraC family compare
Pf6N2E2_5499 +0.4 0.6 hypothetical protein compare
Pf6N2E2_1442 +0.4 1.7 Transcriptional regulators of sugar metabolism compare
Pf6N2E2_1419 +0.4 2.6 Type I secretion outer membrane protein, TolC precursor compare
Pf6N2E2_264 +0.4 0.9 Transcriptional regulator, TetR family compare
Pf6N2E2_747 +0.4 1.9 Transcriptional regulator in custer with plant-induced nitrilase compare
Pf6N2E2_2103 +0.4 1.2 hypothetical protein compare
Pf6N2E2_5318 +0.4 3.8 Cytochrome O ubiquinol oxidase subunit I (EC 1.10.3.-) compare
Pf6N2E2_5874 +0.4 3.8 TonB-dependent receptor; Outer membrane receptor for ferrienterochelin and colicins compare
Pf6N2E2_6089 +0.4 2.1 ThiJ/PfpI family protein compare
Pf6N2E2_1354 +0.4 1.4 FIG026291: Hypothetical periplasmic protein compare
Pf6N2E2_1735 +0.4 0.6 PhnB protein compare
Pf6N2E2_4096 +0.4 3.1 Sulfate transporter family protein in cluster with carbonic anhydrase compare
Pf6N2E2_347 +0.4 1.8 Endoribonuclease L-PSP compare
Pf6N2E2_1893 +0.4 1.2 hypothetical protein compare
Pf6N2E2_116 +0.4 1.3 Glycosyltransferase compare
Pf6N2E2_231 +0.4 2.2 FIG00956946: hypothetical protein compare
Pf6N2E2_3696 +0.4 0.6 hypothetical protein compare
Pf6N2E2_3351 +0.4 1.8 tRNA pseudouridine synthase B (EC 4.2.1.70) compare
Pf6N2E2_4381 +0.4 2.0 hypothetical protein compare
Pf6N2E2_1994 +0.4 1.4 putative lipoprotein compare
Pf6N2E2_5704 +0.4 2.0 Transcriptional regulatory protein PhoP compare
Pf6N2E2_251 +0.4 1.8 transcriptional regulator compare
Pf6N2E2_505 +0.4 1.6 hypothetical protein compare
Pf6N2E2_6047 +0.4 0.9 hypothetical protein compare
Pf6N2E2_493 +0.4 1.5 YD repeat protein compare
Pf6N2E2_425 +0.4 1.5 FIG00957304: hypothetical protein compare
Pf6N2E2_2285 +0.4 0.5 FIG00954079: hypothetical protein compare
Pf6N2E2_1339 +0.4 1.2 Acetylornithine deacetylase (EC 3.5.1.16) compare
Pf6N2E2_3587 +0.4 1.5 Azurin compare
Pf6N2E2_4152 +0.4 2.4 alginate biosynthesis transcriptional regulatory protein AlgB compare
Pf6N2E2_6071 +0.4 1.6 FIG139991: Putative thiamine pyrophosphate-requiring enzyme compare
Pf6N2E2_3903 +0.4 2.6 Ribosomal protein S6 glutaminyl transferase compare
Pf6N2E2_217 +0.4 1.5 Signal transduction histidine kinase compare
Pf6N2E2_1614 +0.4 1.9 Transcriptional regulator, LysR family compare
Pf6N2E2_5844 +0.4 1.7 Membrane protein, putative compare
Pf6N2E2_205 +0.4 1.7 hypothetical protein compare
Pf6N2E2_1238 +0.4 1.1 hypothetical protein compare
Pf6N2E2_5088 +0.4 1.2 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf6N2E2_4397 +0.4 1.5 Xanthine phosphoribosyltransferase (EC 2.4.2.22) compare
Pf6N2E2_3923 +0.4 1.8 Acyl-CoA dehydrogenase; probable dibenzothiophene desulfurization enzyme compare
Pf6N2E2_5328 +0.4 1.6 FIG00958649: hypothetical protein compare
Pf6N2E2_5425 +0.4 0.5 hypothetical protein compare
Pf6N2E2_3871 +0.4 3.2 FIG00553872: hypothetical protein compare
Pf6N2E2_3984 +0.4 2.9 T1SS associated transglutaminase-like cysteine proteinase LapP compare
Pf6N2E2_338 +0.4 1.0 Transcriptional regulator, AraC family compare
Pf6N2E2_630 +0.4 1.5 Epimerase KguE compare
Pf6N2E2_1758 +0.4 0.7 Transcriptional regulator, MarR family compare
Pf6N2E2_2055 +0.4 1.5 StbE replicon stabilization toxin compare
Pf6N2E2_5872 +0.4 2.6 Sensor histidine kinase/response regulator compare
Pf6N2E2_1367 +0.4 2.3 FIG002188: hypothetical protein compare
Pf6N2E2_3956 +0.4 1.3 FIG00960943: hypothetical protein compare
Pf6N2E2_2182 +0.4 1.6 hypothetical protein compare
Pf6N2E2_681 +0.4 1.4 4-hydroxyproline epimerase (EC 5.1.1.8) compare
Pf6N2E2_2357 +0.4 1.4 Invasion protein iagA compare
Pf6N2E2_988 +0.4 1.3 4-hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27) compare
Pf6N2E2_77 +0.4 1.7 O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48) compare
Pf6N2E2_1618 +0.4 2.2 ABC-type amino acid transport, signal transduction systems, periplasmic component/domain compare
Pf6N2E2_5188 +0.4 1.8 Ribosomal-protein-S18p-alanine acetyltransferase (EC 2.3.1.-) compare
Pf6N2E2_1677 +0.4 2.0 Chalcone synthase (EC 2.3.1.74) compare
Pf6N2E2_4311 +0.4 1.9 Protein serine/threonine phosphatase PrpC, regulation of stationary phase compare
Pf6N2E2_5505 +0.4 1.2 hypothetical protein compare
Pf6N2E2_1316 +0.4 1.2 P-hydroxyphenylacetate hydroxylase C1:reductase component compare
Pf6N2E2_2275 +0.4 2.4 Thiamin biosynthesis lipoprotein ApbE compare
Pf6N2E2_2123 +0.4 1.3 hypothetical protein compare
Pf6N2E2_1006 +0.4 1.2 Ribose ABC transport system, periplasmic ribose-binding protein RbsB (TC 3.A.1.2.1) compare
Pf6N2E2_2927 +0.4 1.3 Putative signal peptide protein compare
Pf6N2E2_1977 +0.4 2.6 DNA topoisomerase III (EC 5.99.1.2) in PFGI-1-like cluster compare
Pf6N2E2_1894 +0.4 1.3 hypothetical protein compare
Pf6N2E2_4994 +0.4 1.3 Nicotinate-nucleotide adenylyltransferase (EC 2.7.7.18) compare
Pf6N2E2_4897 +0.4 1.4 hypothetical protein compare


Specific Phenotypes

None in this experiment

For Pseudomonas fluorescens FW300-N2E2 in stress experiments

For stress sodium carbonate monohydrate across organisms